SRR3549612_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549612_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1693509
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC	10533	0.6219630365117634	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGCT	8189	0.48355219842351005	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC	6496	0.3835822543606205	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC	5698	0.33646115845856145	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGC	5263	0.31077484678262707	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTC	4966	0.29323729605216153	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCG	4282	0.2528477852789681	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2431	0.14354810042344032	No Hit
CGCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTG	2246	0.1326240368371234	No Hit
TCCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTG	2111	0.12465242286872995	No Hit
GCCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTG	1777	0.10493005942100102	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTTA	25	3.8915903E-5	45.000004	40
TCTAGCG	55	6.184564E-11	40.909092	1
CGCGCAT	45	1.9292202E-8	40.0	36
TATTGCG	85	0.0	39.705883	1
TTTGGGA	11100	0.0	38.85811	4
GTTTGCG	390	0.0	38.653847	1
TTGGGAT	5460	0.0	38.365383	5
TACGGGA	270	0.0	38.333332	4
CGTTCGG	200	0.0	38.250004	2
CTTGTTG	820	0.0	38.140244	1
TTGGGAC	3865	0.0	38.130657	5
GGGCGAT	2415	0.0	38.012424	7
TCCGTTG	125	0.0	37.800003	1
TTTGGGC	3070	0.0	37.52443	4
CGGTAGG	210	0.0	37.5	31
ACCCGTT	30	1.1401503E-4	37.499996	39
CCGTTAT	30	1.1401503E-4	37.499996	41
TAGTCGA	30	1.1401503E-4	37.499996	38
GTACGCG	30	1.1401503E-4	37.499996	1
TTTCGCG	440	0.0	37.329544	1