##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549612_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1693509
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.386966942602605	33.0	31.0	34.0	31.0	34.0
2	32.60382082409955	34.0	31.0	34.0	31.0	34.0
3	32.66608739605163	34.0	31.0	34.0	31.0	34.0
4	36.05241660953676	37.0	35.0	37.0	35.0	37.0
5	36.09088466609862	37.0	35.0	37.0	35.0	37.0
6	35.68173006461731	37.0	35.0	37.0	35.0	37.0
7	36.039874013069905	37.0	35.0	37.0	35.0	37.0
8	36.03276982879925	37.0	36.0	37.0	35.0	37.0
9	37.88391204298294	39.0	38.0	39.0	35.0	39.0
10	37.58464407334121	39.0	37.0	39.0	35.0	39.0
11	37.561583079865535	39.0	37.0	39.0	35.0	39.0
12	37.33240685464323	39.0	37.0	39.0	35.0	39.0
13	37.218850918418504	39.0	37.0	39.0	34.0	39.0
14	38.218755849540806	40.0	38.0	41.0	33.0	41.0
15	38.3237632631418	40.0	38.0	41.0	33.0	41.0
16	38.48620763160987	40.0	38.0	41.0	34.0	41.0
17	38.48739097341674	40.0	38.0	41.0	34.0	41.0
18	38.454478836545896	40.0	38.0	41.0	34.0	41.0
19	38.485075071936436	40.0	38.0	41.0	34.0	41.0
20	38.41701815579368	40.0	38.0	41.0	34.0	41.0
21	38.35856378678826	40.0	37.0	41.0	34.0	41.0
22	38.39179006429845	40.0	37.0	41.0	34.0	41.0
23	38.35968867009269	40.0	37.0	41.0	34.0	41.0
24	38.32724124879171	40.0	37.0	41.0	34.0	41.0
25	38.23139056243575	40.0	37.0	41.0	34.0	41.0
26	38.16135019063967	40.0	37.0	41.0	34.0	41.0
27	38.117642716985856	40.0	37.0	41.0	34.0	41.0
28	38.03611731617606	40.0	37.0	41.0	34.0	41.0
29	38.03192070428914	40.0	37.0	41.0	34.0	41.0
30	37.94944933862176	40.0	36.0	41.0	34.0	41.0
31	37.845055444051376	40.0	36.0	41.0	33.0	41.0
32	37.80152216492502	40.0	36.0	41.0	33.0	41.0
33	37.72780835531432	40.0	36.0	41.0	33.0	41.0
34	37.675234084967954	40.0	36.0	41.0	33.0	41.0
35	37.636159004764664	40.0	36.0	41.0	33.0	41.0
36	37.52272707142389	40.0	36.0	41.0	33.0	41.0
37	37.41867152757972	40.0	35.0	41.0	32.0	41.0
38	37.42349642074533	40.0	35.0	41.0	33.0	41.0
39	37.405550250987744	40.0	35.0	41.0	33.0	41.0
40	37.28235870018996	40.0	35.0	41.0	32.0	41.0
41	37.13004477685091	39.0	35.0	41.0	32.0	41.0
42	37.12206666749335	39.0	35.0	41.0	32.0	41.0
43	37.00876523242569	39.0	35.0	41.0	32.0	41.0
44	36.92166324477756	39.0	35.0	41.0	31.0	41.0
45	36.956350394358694	39.0	35.0	41.0	32.0	41.0
46	36.85526619581	39.0	35.0	41.0	31.0	41.0
47	36.72124446932375	39.0	35.0	41.0	31.0	41.0
48	36.643217721311196	39.0	35.0	41.0	31.0	41.0
49	36.61502596088949	39.0	35.0	40.0	31.0	41.0
50	36.517664801308996	39.0	35.0	40.0	31.0	41.0
51	35.50927393949486	37.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	31.0
10	40.0
11	33.0
12	28.0
13	23.0
14	23.0
15	37.0
16	86.0
17	110.0
18	225.0
19	456.0
20	812.0
21	1382.0
22	2099.0
23	2951.0
24	4350.0
25	6291.0
26	8654.0
27	10688.0
28	13075.0
29	16202.0
30	20569.0
31	27351.0
32	36907.0
33	55907.0
34	107637.0
35	113246.0
36	118311.0
37	191893.0
38	370888.0
39	582828.0
40	371.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.841728623821897	4.507445782691441	53.35867716085359	16.292148432633073
2	19.379229753133878	5.8185105600265485	53.08793753089	21.71432215594957
3	21.11036906210714	5.927160705966132	51.13666357840436	21.825806653522363
4	19.247786696143923	4.819933050252464	51.17663974623105	24.75564050737256
5	19.753246070732423	5.638411133333215	48.3726983440891	26.235644451845253
6	21.524952037455957	7.51569669839369	51.88694007531108	19.07241118883927
7	82.19495733415057	2.3512718267219133	10.321409570306388	5.132361268821128
8	82.54848365140073	4.093630444243284	7.7831295847852004	5.574756319570785
9	76.2452399131035	6.2079977136230164	10.374140320482502	7.172622052790981
10	35.83636107041651	33.60401391430456	16.70779428984434	13.851830725434585
11	31.260890848528117	23.6983092502018	25.593722855916322	19.44707704535376
12	29.803679815105795	20.378043458877396	29.190869372409594	20.627407353607214
13	27.246563201022255	21.046596150359992	30.991036953449907	20.715803695167846
14	20.379224438724567	24.300608972258193	32.409748043854506	22.910418545162734
15	17.862202090452428	24.952686994872774	35.84386029244604	21.341250622228756
16	22.27316181963013	24.20430006572153	31.08114571578893	22.44139239885941
17	22.645288569473205	25.07645368285613	28.826064697618968	23.452193050051697
18	22.69069724459687	24.38097465085807	30.165295844309064	22.763032260235995
19	24.065771129648557	25.7413453368125	28.69373590574364	21.499147627795306
20	24.72989514670427	27.805284766718096	29.296212774777104	18.16860731180053
21	23.981862511507174	26.42206212072094	30.252275010053093	19.343800357718795
22	21.353709959616392	24.057386172733654	31.340784135189125	23.248119732460825
23	21.576797052746695	25.291805357987467	30.404798557315022	22.72659903195082
24	22.712604420761863	24.138460439241836	28.535779851184728	24.613155288811576
25	22.721284622638557	25.84137432986775	25.679993433752053	25.757347613741643
26	21.49088076886512	26.240427420226286	26.980016049516127	25.288675761392472
27	20.838979893227613	27.06185795292496	29.373389807789625	22.725772346057802
28	17.759338745763973	27.455773780948316	29.40757917436518	25.377308298922536
29	20.811226866819133	26.365552235033885	27.714113122516622	25.109107775630363
30	22.80259508511617	26.322328372627485	27.422529198250494	23.452547344005847
31	21.649545411332326	28.898517811242808	26.00600292056316	23.4459338568617
32	24.354520702281476	28.25051416910096	24.485727563301996	22.909237565315564
33	23.362733826628617	27.69698891473266	24.62342981348195	24.31684744515677
34	21.586008695554614	26.6682373698634	27.97274770904672	23.773006225535266
35	22.2356657094825	26.849222531442113	25.121508064025644	25.793603695049743
36	22.49465458996675	30.145573480861337	24.569399985473947	22.790371943697966
37	20.015423596804034	32.1165697967947	26.049108684984844	21.81889792141642
38	20.630064558263346	28.299406734773775	25.24964437744352	25.820884329519362
39	20.76953827821405	25.010318811414646	26.165376150938673	28.05476675943263
40	23.122581574706718	24.391308224520802	28.02896234977198	24.457147851000496
41	21.049902893932064	27.806229550595834	26.179725056081782	24.96414249939032
42	22.34148150378888	26.399505405640006	27.434220898737472	23.824792191833644
43	21.12855615175355	26.715535612742535	27.446857383102184	24.709050852401727
44	21.269447047520856	24.561841714452065	27.10655804014032	27.062153197886758
45	21.343199238976588	22.85934116677266	25.524812681833993	30.27264691241676
46	21.50859546657266	25.349909566468202	27.269297063080266	25.87219790387887
47	18.487235674566833	27.118604034581452	30.607100405135135	23.78705988571658
48	19.23881124930544	26.90880296473181	27.9565682851405	25.895817500822254
49	21.05681162603801	24.297184130701403	29.81430863373032	24.83169560953027
50	20.344503631217787	24.436775948636825	26.791236420946095	28.427483999199293
51	18.951833146443274	24.358772229731287	25.222540889950984	31.466853733874455
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	187.0
1	921.5
2	1656.0
3	3234.0
4	4812.0
5	3422.0
6	2032.0
7	2111.0
8	2190.0
9	2277.5
10	2365.0
11	2314.0
12	2263.0
13	2336.0
14	2409.0
15	2289.0
16	2169.0
17	2347.5
18	2526.0
19	2733.0
20	2940.0
21	3572.0
22	4204.0
23	5321.5
24	6439.0
25	7729.0
26	11732.5
27	14446.0
28	16804.5
29	19163.0
30	22694.0
31	26225.0
32	30939.5
33	35654.0
34	39470.0
35	43286.0
36	47860.5
37	52435.0
38	57830.0
39	63225.0
40	67291.0
41	71357.0
42	80415.5
43	89474.0
44	99650.0
45	109826.0
46	136550.0
47	163274.0
48	181978.0
49	200682.0
50	200142.5
51	199603.0
52	168372.0
53	137141.0
54	118499.5
55	99858.0
56	91270.0
57	82682.0
58	77535.5
59	72389.0
60	65152.5
61	57916.0
62	49530.0
63	41144.0
64	33412.5
65	25681.0
66	20900.5
67	16120.0
68	13100.0
69	10080.0
70	8153.0
71	6226.0
72	5394.0
73	4562.0
74	3618.5
75	2149.0
76	1623.0
77	1223.0
78	823.0
79	595.0
80	367.0
81	292.5
82	218.0
83	156.0
84	94.0
85	55.5
86	17.0
87	16.5
88	16.0
89	13.0
90	10.0
91	8.0
92	6.0
93	3.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1693509.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.518896084854433
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.5541151173208	18.314957779329905
2	13.122827933259392	7.222514764695806
3	5.619532034875686	4.639299543397637
4	2.910619797634759	3.2038817501452392
5	1.7319573874163952	2.383077768385387
6	1.1762728941067084	1.9421838924212096
7	0.8104239599008893	1.5611380916032167
8	0.6060593641789114	1.3342467731273926
9	0.5234371525499801	1.29639713471775
>10	6.490724035673799	42.7503747453272
>50	0.36113566204969655	6.173111451727297
>100	0.08334474856945667	4.262547727420405
>500	0.005643130092103165	1.1652328811747963
>1k	0.0028215650460515825	1.5901376285569022
>5k	8.681738603235639E-4	1.5317834061514597
>10k+	2.1704346508089097E-4	0.629114661818347
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC	10533	0.6219630365117634	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGCT	8189	0.48355219842351005	Illumina Single End Adapter 1 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC	6496	0.3835822543606205	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTGC	5698	0.33646115845856145	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGC	5263	0.31077484678262707	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTC	4966	0.29323729605216153	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCG	4282	0.2528477852789681	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2431	0.14354810042344032	No Hit
CGCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTG	2246	0.1326240368371234	No Hit
TCCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTG	2111	0.12465242286872995	No Hit
GCCTGTCTCTTATACACATCTGACGCGAACCAGATCGTATGCCGTCTTCTG	1777	0.10493005942100102	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.180979847169398E-4	0.0	0.0	0.5235874152425526	0.0
2	1.180979847169398E-4	0.0	0.0	2.002292281883356	0.0
3	1.180979847169398E-4	0.0	0.0	2.768630104711578	0.0
4	1.771469770754097E-4	0.0	0.0	3.674736892452299	0.0
5	1.771469770754097E-4	0.0	0.0	5.305847208370313	0.0
6	1.771469770754097E-4	0.0	0.0	6.11098021917805	0.0
7	1.771469770754097E-4	0.0	0.0	6.927686832488047	0.0
8	1.771469770754097E-4	0.0	0.0	8.14858379849177	0.0
9	1.771469770754097E-4	0.0	0.0	8.656464181766971	0.0
10	1.771469770754097E-4	0.0	0.0	10.024688383705076	0.0
11	2.361959694338796E-4	0.0	0.0	12.211095423762142	0.0
12	2.361959694338796E-4	0.0	0.0	14.69333791553514	0.0
13	2.361959694338796E-4	0.0	0.0	15.371633690756884	0.0
14	2.361959694338796E-4	0.0	0.0	15.64042470397264	0.0
15	2.361959694338796E-4	0.0	0.0	16.091086613652482	0.0
16	2.361959694338796E-4	0.0	0.0	16.88535460986626	0.0
17	2.361959694338796E-4	0.0	0.0	17.93577713493108	0.0
18	2.361959694338796E-4	0.0	0.0	19.001139055062595	0.0
19	2.361959694338796E-4	0.0	0.0	19.91828800437435	0.0
20	2.361959694338796E-4	0.0	0.0	20.927966724711826	0.0
21	2.361959694338796E-4	0.0	0.0	22.293297526024368	0.0
22	2.361959694338796E-4	0.0	0.0	23.81168331553006	0.0
23	2.361959694338796E-4	0.0	0.0	25.074446017115942	0.0
24	2.361959694338796E-4	0.0	0.0	25.995964591862222	0.0
25	2.361959694338796E-4	0.0	0.0	26.861799966814466	0.0
26	2.361959694338796E-4	0.0	0.0	27.591940757326945	0.0
27	2.361959694338796E-4	0.0	0.0	28.39370797557025	0.0
28	2.361959694338796E-4	0.0	0.0	29.171206057954223	0.0
29	2.361959694338796E-4	0.0	0.0	30.02924696591515	0.0
30	2.361959694338796E-4	0.0	0.0	30.960331477423505	0.0
31	2.361959694338796E-4	0.0	0.0	31.827879273154142	0.0
32	2.361959694338796E-4	0.0	0.0	32.63401611683197	0.0
33	2.952449617923495E-4	0.0	0.0	33.38476500567756	0.0
34	2.952449617923495E-4	0.0	0.0	34.2167653080084	0.0
35	2.952449617923495E-4	0.0	0.0	35.07297569720622	0.0
36	3.542939541508194E-4	0.0	0.0	35.885017440119896	0.0
37	3.542939541508194E-4	0.0	0.0	36.69782681993423	0.0
38	4.723919388677592E-4	0.0	0.0	37.57511769940402	0.0
39	4.723919388677592E-4	0.0	0.0	39.03286017375756	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCCGTTA	25	3.8915903E-5	45.000004	40
TCTAGCG	55	6.184564E-11	40.909092	1
CGCGCAT	45	1.9292202E-8	40.0	36
TATTGCG	85	0.0	39.705883	1
TTTGGGA	11100	0.0	38.85811	4
GTTTGCG	390	0.0	38.653847	1
TTGGGAT	5460	0.0	38.365383	5
TACGGGA	270	0.0	38.333332	4
CGTTCGG	200	0.0	38.250004	2
CTTGTTG	820	0.0	38.140244	1
TTGGGAC	3865	0.0	38.130657	5
GGGCGAT	2415	0.0	38.012424	7
TCCGTTG	125	0.0	37.800003	1
TTTGGGC	3070	0.0	37.52443	4
CGGTAGG	210	0.0	37.5	31
ACCCGTT	30	1.1401503E-4	37.499996	39
CCGTTAT	30	1.1401503E-4	37.499996	41
TAGTCGA	30	1.1401503E-4	37.499996	38
GTACGCG	30	1.1401503E-4	37.499996	1
TTTCGCG	440	0.0	37.329544	1
>>END_MODULE