FastQCFastQC Report
Sat 18 Jun 2016
SRR3549605_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549605_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539633
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC52500.972883422622412RNA PCR Primer, Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGCT35560.6589663715895804TruSeq Adapter, Index 7 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC26930.49904286802326764Illumina PCR Primer Index 3 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC25480.47217275444607726Illumina PCR Primer Index 3 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG10650.19735635144626068TruSeq Adapter, Index 7 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG10590.19624448467754937TruSeq Adapter, Index 7 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9970.184755194734199No Hit
GCCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG7360.13638899029525622Illumina PCR Primer Index 3 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG7090.13138558983605525TruSeq Adapter, Index 7 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCT5680.10525672077133905No Hit
CGTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC5560.10303298723391638No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAATA253.8880447E-545.00000419
GCGAAGT302.1633532E-645.0000041
CGACCGA253.8880447E-545.00000425
TCTAGCG253.8880447E-545.0000041
GACCATA253.8880447E-545.0000049
AATGCGC253.8880447E-545.00000424
CGCTCTA253.8880447E-545.00000428
ACTAGCG253.8880447E-545.00000418
TAACCCG253.8880447E-545.00000418
GTCACTA302.1633532E-645.00000425
CGCTAAT253.8880447E-545.00000415
TATTAGG253.8880447E-545.0000042
CTCGCTA253.8880447E-545.00000413
TATAGTG253.8880447E-545.00000438
ACGACCG253.8880447E-545.00000424
TTTAGCG700.045.01
CGGGTAT800.045.06
CGGGTAC351.2103192E-745.06
TCGCAAC207.0299575E-445.044
ATAGGGT207.0299575E-445.024
Produced by FastQC (version 0.11.5)