##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549605_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	539633
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47832137767705	33.0	31.0	34.0	31.0	34.0
2	32.71001217494112	34.0	31.0	34.0	31.0	34.0
3	32.76881880833826	34.0	31.0	34.0	31.0	34.0
4	36.11778189992087	37.0	35.0	37.0	35.0	37.0
5	36.15206816484537	37.0	35.0	37.0	35.0	37.0
6	35.80673346515132	37.0	35.0	37.0	35.0	37.0
7	36.10494169185354	37.0	35.0	37.0	35.0	37.0
8	36.10939657137351	37.0	37.0	37.0	35.0	37.0
9	37.96761132102744	39.0	39.0	39.0	35.0	39.0
10	37.68329957582283	39.0	37.0	39.0	35.0	39.0
11	37.62862723369401	39.0	37.0	39.0	35.0	39.0
12	37.4041505986476	39.0	37.0	39.0	35.0	39.0
13	37.30115837986187	39.0	37.0	39.0	34.0	39.0
14	38.5411659405559	40.0	38.0	41.0	35.0	41.0
15	38.60245018373598	40.0	38.0	41.0	35.0	41.0
16	38.67964709348761	40.0	38.0	41.0	35.0	41.0
17	38.63904357220555	40.0	38.0	41.0	35.0	41.0
18	38.598312186245096	40.0	38.0	41.0	35.0	41.0
19	38.618201629626064	40.0	38.0	41.0	35.0	41.0
20	38.58455839431614	40.0	38.0	41.0	35.0	41.0
21	38.49457316361305	40.0	38.0	41.0	34.0	41.0
22	38.46387822835149	40.0	37.0	41.0	34.0	41.0
23	38.426986488965646	40.0	37.0	41.0	34.0	41.0
24	38.40605745015594	40.0	37.0	41.0	34.0	41.0
25	38.32194843532549	40.0	37.0	41.0	34.0	41.0
26	38.25546992122424	40.0	37.0	41.0	34.0	41.0
27	38.22113732851771	40.0	37.0	41.0	34.0	41.0
28	38.1738811377362	40.0	37.0	41.0	34.0	41.0
29	38.15984011355866	40.0	37.0	41.0	34.0	41.0
30	38.09448829111637	40.0	37.0	41.0	34.0	41.0
31	38.01710792334791	40.0	36.0	41.0	34.0	41.0
32	37.95962811762809	40.0	36.0	41.0	34.0	41.0
33	37.90505769661974	40.0	36.0	41.0	34.0	41.0
34	37.88661738626066	40.0	36.0	41.0	34.0	41.0
35	37.85273880581803	40.0	36.0	41.0	34.0	41.0
36	37.756036046720645	40.0	36.0	41.0	34.0	41.0
37	37.675850068472464	40.0	36.0	41.0	33.0	41.0
38	37.642792416327396	40.0	36.0	41.0	33.0	41.0
39	37.62671111662927	40.0	36.0	41.0	33.0	41.0
40	37.53099977206731	40.0	35.0	41.0	33.0	41.0
41	37.43699514299533	40.0	35.0	41.0	33.0	41.0
42	37.447861787548206	40.0	35.0	41.0	33.0	41.0
43	37.34788458081696	39.0	35.0	41.0	33.0	41.0
44	37.2199272468511	39.0	35.0	41.0	33.0	41.0
45	37.24033000205695	39.0	35.0	41.0	33.0	41.0
46	37.167552762710955	39.0	35.0	41.0	33.0	41.0
47	37.11355495308849	39.0	35.0	41.0	33.0	41.0
48	37.08787453695382	39.0	35.0	41.0	33.0	41.0
49	37.07392246211777	39.0	35.0	41.0	33.0	41.0
50	37.028209913033486	39.0	35.0	41.0	33.0	41.0
51	36.12854847646456	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	9.0
10	11.0
11	13.0
12	12.0
13	7.0
14	5.0
15	9.0
16	15.0
17	38.0
18	61.0
19	108.0
20	175.0
21	301.0
22	464.0
23	704.0
24	1123.0
25	1620.0
26	2350.0
27	2950.0
28	3422.0
29	4255.0
30	5427.0
31	7176.0
32	9630.0
33	14520.0
34	29855.0
35	45278.0
36	35901.0
37	58120.0
38	114694.0
39	201235.0
40	141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.346854250944624	4.47711685534428	54.42031899457594	18.755709899135155
2	19.211204652050558	4.410034226965363	53.52878715719758	22.8499739637865
3	20.6777569199808	4.299774105734824	52.831832004343696	22.190636969940684
4	20.054370284989982	4.673546651149948	50.8554888229593	24.41659424090076
5	18.17272109007418	6.5644614024716805	49.53366454608965	25.72915296136448
6	21.58466958099301	6.441229502272841	52.378190362709475	19.59591055402468
7	81.71868658884834	2.450369047111648	10.54420318994576	5.286741174094246
8	82.51422726186131	3.2618464771427993	8.035461137476767	6.188465123519133
9	76.69990530601353	5.498181171277516	11.417759847896626	6.384153674812326
10	37.95894617267662	28.931885188637462	18.408992778425336	14.700175860260584
11	29.79487911228557	25.042204609429003	25.94114889193211	19.221767386353317
12	28.42561518661757	21.415665832148886	29.927932502274697	20.230786478958848
13	23.902541171499887	24.68807504359444	30.00001853111281	21.409365253792856
14	19.481202965719294	27.18458656160761	29.440934857579133	23.893275615093962
15	17.019529939792413	26.36736448660478	35.46132278789473	21.151782785708065
16	19.73952667831656	24.244996136262976	32.84157936968273	23.17389781573773
17	19.391327068581795	25.478612316148197	29.25358530705127	25.876475308218733
18	20.371808247457068	25.771033276319276	30.609136209238503	23.248022266985156
19	22.382433987543386	25.97672862853087	28.934664855559237	22.7061725283665
20	26.17408497997713	26.263590254858393	28.58109122310904	18.981233542055435
21	25.671335889391493	26.067716392437084	28.280145951044506	19.98080176712692
22	22.177479879844263	25.169513354446448	27.909709005935518	24.74329775977377
23	21.82168251385664	25.496958117831937	27.77294939338402	24.9084099749274
24	23.3553174101658	23.219484353254895	29.989270485681935	23.43592775089737
25	21.047081998321083	25.41597715484413	28.039426795618503	25.497514051216292
26	19.533831326104963	27.55780317363838	29.177422433394547	23.73094306686211
27	20.460942900082095	28.723039547247854	29.168342188116736	21.64767536455332
28	19.51400303539628	25.81680512496456	31.64335761526815	23.025834224371007
29	19.704873497358392	25.46656709282049	31.254574868475427	23.57398454134569
30	21.31003848912131	26.40498264561285	28.62797493852303	23.657003926742806
31	21.883205808392	28.44970563327298	26.175752780130203	23.491335778204817
32	21.46106705853793	26.713155051674008	27.557432551382142	24.268345338405915
33	21.613392805851383	26.5322913906303	25.678007090003764	26.17630871351456
34	19.501587189812337	25.601658905218915	29.248396595463955	25.648357309504792
35	18.859113508625306	24.112313368530096	29.617536362676116	27.411036760168482
36	20.90735740771969	26.966290052683956	27.796483906655077	24.329868632941277
37	20.684428120593072	28.51159955006458	28.821069134022565	21.982903195319782
38	20.1344246923372	26.075128837561824	27.336726997792944	26.45371947230803
39	19.918166605822847	25.08074932407766	29.565093313418565	25.435990756680933
40	21.37749173975646	24.822240300352277	28.7754825965054	25.024785363385853
41	17.72278567100233	25.135416106872633	28.08445739975131	29.057340822373725
42	20.210402254865805	24.31430249817932	26.727424008539135	28.74787123841574
43	22.054618601901662	23.606969922150796	27.71995041074212	26.61846106520543
44	20.76244410553098	23.29360880450232	29.342164026291943	26.601783063674755
45	20.734647436313196	23.57954387518925	27.712167343361138	27.973641345136418
46	20.578615466437373	24.957888046135057	29.70908005996668	24.754416427460885
47	18.21237767149155	25.26531920768374	31.054624161235505	25.4676789595892
48	18.987534120411464	22.383545854312096	31.351307277353314	27.277612747923126
49	20.07512513133926	22.013294220331225	32.248027826318996	25.663552822010516
50	20.214108477428177	21.835395537337412	29.67479750126475	28.275698483969663
51	18.034849610753977	22.509557421432714	27.169205737973773	32.28638722983954
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	112.0
1	333.0
2	554.0
3	1179.0
4	1804.0
5	1263.0
6	722.0
7	753.5
8	785.0
9	781.5
10	778.0
11	823.0
12	868.0
13	831.0
14	794.0
15	894.0
16	994.0
17	964.0
18	934.0
19	1005.0
20	1076.0
21	1317.5
22	1559.0
23	1796.0
24	2033.0
25	2348.0
26	3585.5
27	4508.0
28	5277.5
29	6047.0
30	7187.5
31	8328.0
32	9687.5
33	11047.0
34	12535.5
35	14024.0
36	15360.0
37	16696.0
38	18294.5
39	19893.0
40	22917.0
41	25941.0
42	28706.0
43	31471.0
44	35856.5
45	40242.0
46	45964.5
47	51687.0
48	58823.0
49	65959.0
50	64464.0
51	62969.0
52	51938.5
53	40908.0
54	34747.0
55	28586.0
56	25059.0
57	21532.0
58	20895.5
59	20259.0
60	19049.5
61	17840.0
62	15402.5
63	12965.0
64	10566.0
65	8167.0
66	6634.0
67	5101.0
68	4326.5
69	3552.0
70	3170.0
71	2788.0
72	2229.5
73	1671.0
74	1210.5
75	623.0
76	496.0
77	384.5
78	273.0
79	201.5
80	130.0
81	77.5
82	25.0
83	52.5
84	80.0
85	49.5
86	19.0
87	11.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	539633.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.453754193205476
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.84084726253425	19.303267922669463
2	13.196168201560118	7.5096105259877195
3	5.495431947419941	4.690970094521264
4	2.7501248633523048	3.1300550744979714
5	1.529025945254015	2.1753264200645694
6	1.0116063260583852	1.727039864517418
7	0.6923014251342391	1.378900220486284
8	0.5345739804770437	1.216850931086178
9	0.38654112287645176	0.9898691486302975
>10	6.072539347471633	43.89465733347335
>50	0.41977969259480846	7.327964123981835
>100	0.06382230436002574	2.9604286110194873
>500	0.0032898095030941105	0.6676081974891223
>1k	0.0032898095030941105	2.044573506668173
>5k	6.579619006188221E-4	0.9828780249068683
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	5250	0.972883422622412	RNA PCR Primer, Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGCT	3556	0.6589663715895804	TruSeq Adapter, Index 7 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	2693	0.49904286802326764	Illumina PCR Primer Index 3 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	2548	0.47217275444607726	Illumina PCR Primer Index 3 (95% over 22bp)
CGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG	1065	0.19735635144626068	TruSeq Adapter, Index 7 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG	1059	0.19624448467754937	TruSeq Adapter, Index 7 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	997	0.184755194734199	No Hit
GCCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG	736	0.13638899029525622	Illumina PCR Primer Index 3 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG	709	0.13138558983605525	TruSeq Adapter, Index 7 (95% over 22bp)
TTCCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCT	568	0.10525672077133905	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC	556	0.10303298723391638	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6813890180919254	0.0
2	0.0	0.0	0.0	2.737045362311052	0.0
3	0.0	0.0	0.0	3.7833119916684117	0.0
4	0.0	0.0	0.0	4.767870015362292	0.0
5	0.0	0.0	0.0	6.299651800390265	0.0
6	0.0	0.0	0.0	7.215459395552163	0.0
7	0.0	0.0	0.0	8.061404695413364	0.0
8	0.0	0.0	0.0	9.412878752781983	0.0
9	0.0	0.0	0.0	10.026999831366874	0.0
10	0.0	0.0	0.0	11.202984250407221	0.0
11	0.0	0.0	0.0	13.308674599218358	0.0
12	0.0	0.0	0.0	15.201813084077513	0.0
13	0.0	0.0	0.0	15.847807676698794	0.0
14	0.0	0.0	0.0	16.084635298434307	0.0
15	0.0	0.0	0.0	16.45618411031201	0.0
16	0.0	0.0	0.0	17.283042363977	0.0
17	0.0	0.0	0.0	18.43808662553995	0.0
18	0.0	0.0	0.0	19.67411185009071	0.0
19	0.0	0.0	0.0	20.515980305133304	0.0
20	0.0	0.0	0.0	21.43716192301064	0.0
21	0.0	0.0	0.0	22.58071689463024	0.0
22	0.0	0.0	0.0	23.827490164611874	0.0
23	1.8531112811855465E-4	0.0	0.0	24.934538843992122	0.0
24	1.8531112811855465E-4	0.0	0.0	25.840339638235616	0.0
25	1.8531112811855465E-4	0.0	0.0	26.58732879568151	0.0
26	1.8531112811855465E-4	0.0	0.0	27.268347191517197	0.0
27	1.8531112811855465E-4	0.0	0.0	27.9115621172167	0.0
28	1.8531112811855465E-4	0.0	0.0	28.624268715960664	0.0
29	1.8531112811855465E-4	0.0	0.0	29.37422285145645	0.0
30	1.8531112811855465E-4	0.0	0.0	30.18625621487196	0.0
31	1.8531112811855465E-4	0.0	0.0	30.93083632765231	0.0
32	1.8531112811855465E-4	0.0	0.0	31.673748640279598	0.0
33	1.8531112811855465E-4	0.0	0.0	32.323264144335134	0.0
34	1.8531112811855465E-4	0.0	0.0	33.01299216319239	0.0
35	1.8531112811855465E-4	0.0	0.0	33.69401055902808	0.0
36	1.8531112811855465E-4	0.0	0.0	34.37817924404178	0.0
37	1.8531112811855465E-4	0.0	0.0	35.04233432721868	0.0
38	1.8531112811855465E-4	0.0	0.0	35.70018883203955	0.0
39	1.8531112811855465E-4	0.0	0.0	36.401035518583924	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATA	25	3.8880447E-5	45.000004	19
GCGAAGT	30	2.1633532E-6	45.000004	1
CGACCGA	25	3.8880447E-5	45.000004	25
TCTAGCG	25	3.8880447E-5	45.000004	1
GACCATA	25	3.8880447E-5	45.000004	9
AATGCGC	25	3.8880447E-5	45.000004	24
CGCTCTA	25	3.8880447E-5	45.000004	28
ACTAGCG	25	3.8880447E-5	45.000004	18
TAACCCG	25	3.8880447E-5	45.000004	18
GTCACTA	30	2.1633532E-6	45.000004	25
CGCTAAT	25	3.8880447E-5	45.000004	15
TATTAGG	25	3.8880447E-5	45.000004	2
CTCGCTA	25	3.8880447E-5	45.000004	13
TATAGTG	25	3.8880447E-5	45.000004	38
ACGACCG	25	3.8880447E-5	45.000004	24
TTTAGCG	70	0.0	45.0	1
CGGGTAT	80	0.0	45.0	6
CGGGTAC	35	1.2103192E-7	45.0	6
TCGCAAC	20	7.0299575E-4	45.0	44
ATAGGGT	20	7.0299575E-4	45.0	24
>>END_MODULE