FastQCFastQC Report
Sat 18 Jun 2016
SRR3549602_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549602_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1466949
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC65600.4471866438437873No Hit
CTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGCT52030.3546817237681746No Hit
TCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC50170.3420023463665063No Hit
GCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTGC44620.30416872024862485No Hit
TCCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTG20990.14308609229087035No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19830.13517852358875462No Hit
CGCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTG16760.11425073400643103No Hit
GCCTGTCTCTTATACACATCTGACGCCTCTCTCATCGTATGCCGTCTTCTG14850.1012305131262232No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAA302.1659125E-645.00000420
TTATCCG207.0339255E-445.01
TCGTTTG4400.039.3750041
CTACGGG1900.039.0789453
TACGGGA4150.039.0361444
TGGGCGA16300.038.23626
TTTGGGA110650.038.2286454
TTGGGAC40550.037.7866825
TGTCACG601.5643309E-1037.5000041
AACCGGT301.1400755E-437.50000438
CGTTATA301.1400755E-437.50000426
ATCGTCG301.1400755E-437.5000041
CGTTTTT14450.037.3702431
CTTTTCG4100.037.3170741
TTGGGAT71600.037.238135
GGGCGAT26050.037.2264867
GGCCGAT4250.037.0588238
TCTGGGA50000.036.814
GGCGATT13750.036.6545458
CTTGGGA47450.036.512124