FastQCFastQC Report
Sat 18 Jun 2016
SRR3549601_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549601_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457575
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGC66531.45396929465115No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCG53981.1796973173796645No Hit
GAATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTC49351.0785117193902638RNA PCR Primer, Index 30 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC27980.6114844561000929RNA PCR Primer, Index 30 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC23980.5240670928263126Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT21490.4696497841883844RNA PCR Primer, Index 30 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC16030.3503250833196744Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATCGGAACT13680.2989673823963285No Hit
GAATGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCT12800.2797355624760968No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTA12230.2672785882095831No Hit
GAACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCT11450.250232202371196No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTC10360.22641097087909087No Hit
TCCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTG7010.1531989291372999RNA PCR Primer, Index 30 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6880.15035786483090205No Hit
CGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTG5700.12456974266513686RNA PCR Primer, Index 30 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAA253.887112E-545.00000419
CGGGTAC502.1827873E-1145.0000046
AATCCCG207.0288294E-445.00000413
GATCGCC253.887112E-545.00000435
CGAAACG207.0288294E-445.00000433
CATGCGT207.0288294E-445.00000411
ACGTTAT207.0288294E-445.00000430
CGTGACA207.0288294E-445.00000444
GGCCGTC207.0288294E-445.0000048
GGCGTAG207.0288294E-445.0000041
GACAACG207.0288294E-445.00000433
GATTTCG207.0288294E-445.00000410
TATTGCG253.887112E-545.0000041
AACGGTT207.0288294E-445.00000436
TAGCAAC207.0288294E-445.00000421
AACGGCG207.0288294E-445.00000414
AACGGCC207.0288294E-445.00000437
ATATCAC1100.045.00000430
TACGCGG253.887112E-545.0000042
AGGCCGT207.0288294E-445.0000047