SRR3549601_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549601_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	457575
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGC	6653	1.45396929465115	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCG	5398	1.1796973173796645	No Hit
GAATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTC	4935	1.0785117193902638	RNA PCR Primer, Index 30 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	2798	0.6114844561000929	RNA PCR Primer, Index 30 (95% over 24bp)
GCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	2398	0.5240670928263126	Illumina Single End Adapter 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGCT	2149	0.4696497841883844	RNA PCR Primer, Index 30 (96% over 25bp)
TCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTGC	1603	0.3503250833196744	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATCGGAACT	1368	0.2989673823963285	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCT	1280	0.2797355624760968	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATCGGAACTCGTA	1223	0.2672785882095831	No Hit
GAACTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCT	1145	0.250232202371196	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTC	1036	0.22641097087909087	No Hit
TCCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTG	701	0.1531989291372999	RNA PCR Primer, Index 30 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	688	0.15035786483090205	No Hit
CGCTGTCTCTTATACACATCTGACGCATCGGAACTCGTATGCCGTCTTCTG	570	0.12456974266513686	RNA PCR Primer, Index 30 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCAA	25	3.887112E-5	45.000004	19
CGGGTAC	50	2.1827873E-11	45.000004	6
AATCCCG	20	7.0288294E-4	45.000004	13
GATCGCC	25	3.887112E-5	45.000004	35
CGAAACG	20	7.0288294E-4	45.000004	33
CATGCGT	20	7.0288294E-4	45.000004	11
ACGTTAT	20	7.0288294E-4	45.000004	30
CGTGACA	20	7.0288294E-4	45.000004	44
GGCCGTC	20	7.0288294E-4	45.000004	8
GGCGTAG	20	7.0288294E-4	45.000004	1
GACAACG	20	7.0288294E-4	45.000004	33
GATTTCG	20	7.0288294E-4	45.000004	10
TATTGCG	25	3.887112E-5	45.000004	1
AACGGTT	20	7.0288294E-4	45.000004	36
TAGCAAC	20	7.0288294E-4	45.000004	21
AACGGCG	20	7.0288294E-4	45.000004	14
AACGGCC	20	7.0288294E-4	45.000004	37
ATATCAC	110	0.0	45.000004	30
TACGCGG	25	3.887112E-5	45.000004	2
AGGCCGT	20	7.0288294E-4	45.000004	7