SRR3549600_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549600_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	803057
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGC	2852	0.35514291015457183	No Hit
CTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGCT	2659	0.3311097468797358	TruSeq Adapter, Index 22 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTC	2596	0.3232647246708515	No Hit
CCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	2407	0.2997296580441986	TruSeq Adapter, Index 20 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCG	2260	0.28142460622346854	No Hit
TCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	2145	0.26710432758820357	TruSeq Adapter, Index 20 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTGC	2082	0.2592593053793193	TruSeq Adapter, Index 20 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1123	0.1398406339774138	No Hit
TCCTGTCTCTTATACACATCTGACGCACGGCTTATCGTATGCCGTCTTCTG	955	0.11892057475372234	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCTT	20	7.032015E-4	45.000004	43
TGGCACG	20	7.032015E-4	45.000004	32
GACGGTC	20	7.032015E-4	45.000004	9
CTCGAGT	20	7.032015E-4	45.000004	27
CAGTCGA	20	7.032015E-4	45.000004	28
ATCATCG	30	2.164681E-6	45.000004	23
TTATCCG	25	3.8897517E-5	45.0	1
TCGTGCG	25	3.8897517E-5	45.0	1
ACGCTCC	25	3.8897517E-5	45.0	25
GCGATAT	25	3.8897517E-5	45.0	9
CGACTAC	25	3.8897517E-5	45.0	45
CGCTTCA	25	3.8897517E-5	45.0	11
CCCGATC	25	3.8897517E-5	45.0	41
TCGCTTG	235	0.0	42.12766	1
GTATCGG	65	0.0	41.53846	2
ACGAGCG	60	3.6379788E-12	41.250004	42
CGTTTTT	865	0.0	41.098267	1
TATTGCG	50	1.0804797E-9	40.5	1
TATCCGG	100	0.0	40.5	2
ACCGGGT	45	1.9270374E-8	40.0	4