SRR3549597_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549597_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	133759
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGCT	701	0.5240768845460866	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	679	0.5076293931623292	No Hit
TCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	452	0.337921186611742	No Hit
GCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	444	0.33194028065401204	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	292	0.21830306745714306	No Hit
CGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG	224	0.16746536681643853	No Hit
TCCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG	209	0.15625116814569487	No Hit
GCCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG	142	0.10616108074970657	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATTC	30	2.1510914E-6	45.000004	43
TTTCGCG	30	2.1510914E-6	45.000004	1
GGCCGAT	30	2.1510914E-6	45.000004	8
TCGGGAC	30	2.1510914E-6	45.000004	5
GCCGATT	30	2.1510914E-6	45.000004	9
GACCTAT	30	2.1510914E-6	45.000004	33
CTTGTCG	25	3.8722832E-5	45.0	1
TGTTTCG	20	7.010927E-4	45.0	1
AACCCCC	20	7.010927E-4	45.0	16
ACTCTCC	20	7.010927E-4	45.0	39
TCGGGTC	20	7.010927E-4	45.0	5
TCGGGCA	25	3.8722832E-5	45.0	5
TTCGTTG	35	1.2011878E-7	45.0	1
TGTATAA	25	3.8722832E-5	45.0	31
ACGGGAG	35	1.2011878E-7	45.0	5
TTTTTCG	35	1.2011878E-7	45.0	1
GCGGGCT	20	7.010927E-4	45.0	5
CGGGATA	50	2.1827873E-11	45.0	6
CGGGAAC	35	1.2011878E-7	45.0	6
ATGCGGG	25	3.8722832E-5	45.0	3