##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549597_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	133759
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.463109024439476	33.0	31.0	34.0	31.0	34.0
2	32.692970192659935	34.0	31.0	34.0	31.0	34.0
3	32.75693598187785	34.0	31.0	34.0	31.0	34.0
4	36.111566324509006	37.0	35.0	37.0	35.0	37.0
5	36.147556426109645	37.0	35.0	37.0	35.0	37.0
6	35.78669846515001	37.0	35.0	37.0	35.0	37.0
7	36.07322871731996	37.0	35.0	37.0	35.0	37.0
8	36.072421295015666	37.0	37.0	37.0	35.0	37.0
9	37.93648277872891	39.0	38.0	39.0	35.0	39.0
10	37.64178858992666	39.0	37.0	39.0	35.0	39.0
11	37.58517931503675	39.0	37.0	39.0	35.0	39.0
12	37.37839696768068	39.0	37.0	39.0	35.0	39.0
13	37.28285947113839	39.0	37.0	39.0	34.0	39.0
14	38.518948257687335	40.0	38.0	41.0	34.0	41.0
15	38.622096457060834	40.0	38.0	41.0	35.0	41.0
16	38.674235004747345	40.0	38.0	41.0	35.0	41.0
17	38.63359474876457	40.0	38.0	41.0	35.0	41.0
18	38.592363878318466	40.0	38.0	41.0	35.0	41.0
19	38.61643702479833	40.0	38.0	41.0	35.0	41.0
20	38.553644988374614	40.0	38.0	41.0	35.0	41.0
21	38.46322864255863	40.0	37.0	41.0	34.0	41.0
22	38.455632891992316	40.0	37.0	41.0	34.0	41.0
23	38.41768404369052	40.0	37.0	41.0	34.0	41.0
24	38.41394597746694	40.0	37.0	41.0	34.0	41.0
25	38.3298619158337	40.0	37.0	41.0	34.0	41.0
26	38.27787289079613	40.0	37.0	41.0	34.0	41.0
27	38.24797583713993	40.0	37.0	41.0	34.0	41.0
28	38.16451977063225	40.0	37.0	41.0	34.0	41.0
29	38.139893390351304	40.0	37.0	41.0	34.0	41.0
30	38.073774474988596	40.0	37.0	41.0	34.0	41.0
31	37.99283786511562	40.0	36.0	41.0	34.0	41.0
32	37.8868337831473	40.0	36.0	41.0	34.0	41.0
33	37.7777719630081	40.0	36.0	41.0	33.0	41.0
34	37.74183419433459	40.0	36.0	41.0	33.0	41.0
35	37.70222564462952	40.0	36.0	41.0	33.0	41.0
36	37.639276609424414	40.0	36.0	41.0	33.0	41.0
37	37.5517086700708	40.0	36.0	41.0	33.0	41.0
38	37.495413392743664	40.0	36.0	41.0	33.0	41.0
39	37.43032618365867	40.0	35.0	41.0	33.0	41.0
40	37.34678787969408	40.0	35.0	41.0	33.0	41.0
41	37.26884919893241	39.0	35.0	41.0	33.0	41.0
42	37.226638955135726	39.0	35.0	41.0	33.0	41.0
43	37.09670377320405	39.0	35.0	41.0	32.0	41.0
44	36.98166104710711	39.0	35.0	41.0	32.0	41.0
45	36.97950792096233	39.0	35.0	41.0	32.0	41.0
46	36.90761743135042	39.0	35.0	41.0	32.0	41.0
47	36.82884142375466	39.0	35.0	41.0	32.0	41.0
48	36.75615846410335	39.0	35.0	41.0	31.0	41.0
49	36.74717962903431	39.0	35.0	41.0	31.0	41.0
50	36.66331237524204	39.0	35.0	41.0	31.0	41.0
51	35.72607450713597	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	1.0
12	2.0
13	1.0
14	4.0
15	2.0
16	5.0
17	13.0
18	10.0
19	37.0
20	56.0
21	94.0
22	121.0
23	231.0
24	315.0
25	474.0
26	695.0
27	898.0
28	1012.0
29	1198.0
30	1426.0
31	1846.0
32	2526.0
33	3776.0
34	7291.0
35	10678.0
36	9276.0
37	14851.0
38	28770.0
39	48113.0
40	32.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.92736189714337	5.3125397169536255	52.88690854447178	17.87318984143123
2	20.403860674795716	4.869205062836893	52.63645810749183	22.09047615487556
3	20.635620780657753	5.075546318378576	52.31797486524271	21.970858035720962
4	18.94003394164131	4.9768613700760325	51.33934912790915	24.743755560373508
5	18.657436135138568	6.350974513864488	50.044482988060615	24.947106362936324
6	21.713679079538572	6.736742948138069	52.4600213817388	19.089556590584557
7	82.57313526566436	2.6435604333166367	9.758595683281126	5.024708617737872
8	83.37980995671319	3.6969474951218237	7.843210550318109	5.080031997846874
9	77.42507046254832	5.9928677696454065	10.253515651283278	6.3285461165229995
10	37.53840863044729	31.162015266262454	17.675819944826145	13.623756158464104
11	30.524301168519504	24.31387794466167	26.365328688163043	18.79649219865579
12	28.982722657914607	21.44752876441959	29.648098445712062	19.92165013195374
13	24.687684567019787	25.001682129800614	30.019662228336035	20.29097107484356
14	20.530207313152758	26.45130421130541	30.47944437383653	22.539044101705304
15	18.420442736563523	25.957879469792687	33.736047667820486	21.88563012582331
16	20.856166687849043	24.347520540673898	32.30436830418888	22.49194446728818
17	19.953797501476537	25.655096105682606	28.616392168003646	25.774714224837208
18	21.90731090992008	25.107843210550318	31.109682339132323	21.87516354039728
19	23.491503375473798	26.587369821843765	28.191747845004823	21.729378957677614
20	26.07450713596842	25.986288773091903	28.909456559932416	19.02974753100726
21	25.053267443686032	27.65496153529856	27.669166186948168	19.62260483406724
22	22.46876845670198	24.577785420046503	28.65676328321832	24.296682840033192
23	21.7241456649646	25.921994034046307	28.128948332448655	24.224911968540436
24	22.779775566503936	23.51393177281529	30.10638536472312	23.599907295957657
25	20.54216912506822	26.019931369104132	27.665428120724588	25.772471385103056
26	20.163129209997084	27.406006324808054	28.430236470069303	24.000627995125562
27	20.269290290746792	26.90435783760345	29.295224994206	23.53112687744376
28	18.17522559229659	27.157051114317543	31.170239011954337	23.49748428143153
29	18.824153888710292	26.136559035279873	30.554205698308152	24.485081377701686
30	20.681225188585444	26.01021239692282	28.60816842231177	24.700393992179965
31	22.280369919033486	28.0482061020193	25.872651559895033	23.798772419052174
32	22.260931974670864	28.28370427410492	25.666310304353352	23.789053446870863
33	21.94543918540061	27.520391151249633	25.48015460641901	25.05401505693075
34	19.844645967747965	26.214310812730357	27.650475855830265	26.290567363691412
35	19.9291262644009	25.830785218190925	28.4900455296466	25.750042987761574
36	20.382179890698943	27.873264602755704	26.136559035279873	25.607996471265487
37	19.717551716146204	29.588289386134765	27.017247437555604	23.676911460163428
38	20.234900081489844	29.900791722426156	25.197556799916264	24.666751396167736
39	20.60347341113495	28.031010997390833	26.579146076151883	24.786369515322335
40	22.195142009135836	23.908671566025465	28.193990684738974	25.702195740099732
41	19.531396018211858	24.994953610598163	27.208636428202965	28.265013942987018
42	21.161940504937984	25.800880688402277	27.18471280437204	25.852466002287695
43	20.974289580514206	26.02665988830658	27.557024200240733	25.44202633093848
44	20.009868494830254	25.655843718927322	27.012761758087304	27.321526028155112
45	20.533197766131625	24.671237075636032	26.62848855030316	28.167076607929186
46	20.431522364850217	25.080929133740533	27.679632772374198	26.807915729035052
47	18.339700506134164	25.76424763941118	30.010690869399443	25.88536098505521
48	18.761354376154127	24.80281700670609	30.279084024252573	26.15674459288721
49	20.184809994093854	24.526947719405797	30.30001719510463	24.98822509139572
50	20.111543896111662	24.017823099754036	28.40930329921725	27.461329704917052
51	18.674631239767045	24.402843920782903	26.514851337106286	30.40767350234377
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	16.0
1	68.0
2	120.0
3	341.0
4	562.0
5	382.5
6	203.0
7	210.0
8	217.0
9	218.0
10	219.0
11	238.5
12	258.0
13	235.0
14	212.0
15	230.5
16	249.0
17	243.0
18	237.0
19	248.5
20	260.0
21	275.0
22	290.0
23	353.0
24	416.0
25	618.5
26	981.5
27	1142.0
28	1277.5
29	1413.0
30	1846.0
31	2279.0
32	2500.0
33	2721.0
34	3189.5
35	3658.0
36	3818.0
37	3978.0
38	4676.0
39	5374.0
40	5955.5
41	6537.0
42	7461.0
43	8385.0
44	9222.0
45	10059.0
46	11029.5
47	12000.0
48	13616.5
49	15233.0
50	14665.5
51	14098.0
52	12135.0
53	10172.0
54	8866.0
55	7560.0
56	6753.0
57	5946.0
58	5641.0
59	5336.0
60	4898.0
61	4460.0
62	3888.0
63	3316.0
64	2757.0
65	2198.0
66	1710.5
67	1223.0
68	1112.0
69	1001.0
70	799.5
71	598.0
72	531.0
73	464.0
74	379.5
75	221.0
76	147.0
77	98.5
78	50.0
79	35.5
80	21.0
81	13.5
82	6.0
83	7.0
84	8.0
85	4.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	133759.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.53831144072549
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.65753516634773	32.36043929754259
2	9.431966965454729	8.401677644121143
3	3.942995266391379	5.2684305355153676
4	2.4977339107664416	4.449794032551081
5	2.049551817907141	4.5641788589926655
6	1.7339779098264345	4.633706890751277
7	1.379796555544365	4.301766610097264
8	1.2606170477053749	4.49166037425519
9	1.050793970524054	4.212053020731315
>10	3.9446738510088295	23.691116111813038
>50	0.030214523114110183	0.9135833850432494
>100	0.016785846174505657	1.679886960877399
>500	0.0033571692349011312	1.0317062777084158
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGCT	701	0.5240768845460866	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	679	0.5076293931623292	No Hit
TCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	452	0.337921186611742	No Hit
GCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTGC	444	0.33194028065401204	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	292	0.21830306745714306	No Hit
CGCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG	224	0.16746536681643853	No Hit
TCCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG	209	0.15625116814569487	No Hit
GCCTGTCTCTTATACACATCTGACGCTCAACCGATCGTATGCCGTCTTCTG	142	0.10616108074970657	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.546505281887574	0.0
2	0.0	0.0	0.0	1.8316524495547963	0.0
3	0.0	0.0	0.0	2.623374875709298	0.0
4	0.0	0.0	0.0	3.388930838298731	0.0
5	0.0	0.0	0.0	4.6501543821350335	0.0
6	0.0	0.0	0.0	5.397020013606561	0.0
7	0.0	0.0	0.0	6.070619547095896	0.0
8	0.0	0.0	0.0	7.1621348843816115	0.0
9	0.0	0.0	0.0	7.707144939779753	0.0
10	0.0	0.0	0.0	8.776231879723982	0.0
11	0.0	0.0	0.0	10.695355078910579	0.0
12	0.0	0.0	0.0	12.515793329794631	0.0
13	0.0	0.0	0.0	13.079493716310678	0.0
14	0.0	0.0	0.0	13.250697149350698	0.0
15	0.0	0.0	0.0	13.592356402186022	0.0
16	0.0	0.0	0.0	14.245770378068018	0.0
17	0.0	0.0	0.0	15.215424756464985	0.0
18	0.0	0.0	0.0	16.259840459333578	0.0
19	0.0	0.0	0.0	16.993249052400213	0.0
20	0.0	0.0	0.0	17.84926621760031	0.0
21	0.0	0.0	0.0	18.939286328396594	0.0
22	0.0	0.0	0.0	20.061453808715676	0.0
23	0.0	0.0	0.0	21.087926793711077	0.0
24	0.0	0.0	0.0	21.890115805291607	0.0
25	0.0	0.0	0.0	22.59810554803789	0.0
26	0.0	0.0	0.0	23.204419889502763	0.0
27	0.0	0.0	0.0	23.83615308128799	0.0
28	0.0	0.0	0.0	24.452186394934174	0.0
29	0.0	0.0	0.0	25.080929133740533	0.0
30	0.0	0.0	0.0	25.842747030106384	0.0
31	0.0	0.0	0.0	26.517094176840438	0.0
32	0.0	0.0	0.0	27.23854095799161	0.0
33	0.0	0.0	0.0	27.865040857063825	0.0
34	0.0	0.0	0.0	28.534154711084863	0.0
35	0.0	0.0	0.0	29.20924947106363	0.0
36	0.0	0.0	0.0	29.844720729072435	0.0
37	0.0	0.0	0.0	30.47869676059181	0.0
38	0.0	0.0	0.0	31.132858349718525	0.0
39	0.0	0.0	0.0	31.775805740174494	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATTC	30	2.1510914E-6	45.000004	43
TTTCGCG	30	2.1510914E-6	45.000004	1
GGCCGAT	30	2.1510914E-6	45.000004	8
TCGGGAC	30	2.1510914E-6	45.000004	5
GCCGATT	30	2.1510914E-6	45.000004	9
GACCTAT	30	2.1510914E-6	45.000004	33
CTTGTCG	25	3.8722832E-5	45.0	1
TGTTTCG	20	7.010927E-4	45.0	1
AACCCCC	20	7.010927E-4	45.0	16
ACTCTCC	20	7.010927E-4	45.0	39
TCGGGTC	20	7.010927E-4	45.0	5
TCGGGCA	25	3.8722832E-5	45.0	5
TTCGTTG	35	1.2011878E-7	45.0	1
TGTATAA	25	3.8722832E-5	45.0	31
ACGGGAG	35	1.2011878E-7	45.0	5
TTTTTCG	35	1.2011878E-7	45.0	1
GCGGGCT	20	7.010927E-4	45.0	5
CGGGATA	50	2.1827873E-11	45.0	6
CGGGAAC	35	1.2011878E-7	45.0	6
ATGCGGG	25	3.8722832E-5	45.0	3
>>END_MODULE