FastQCFastQC Report
Sat 18 Jun 2016
SRR3549595_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549595_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437535
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC36030.8234769789845383TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT31880.7286274240917869Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC27620.6312637846115168TruSeq Adapter, Index 27 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC23370.5341286982755665TruSeq Adapter, Index 27 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG11060.2527797776177906No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC10920.2495800335973122No Hit
CGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG8860.2024980858674163No Hit
GCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG7920.18101409030134732TruSeq Adapter, Index 27 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7200.16455826391031575No Hit
TGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG7000.15998720102391809No Hit
TTCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCT6200.14170294947832746No Hit
TTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG4730.10810563726330466No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTC4540.10376312752122688No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGGG502.1827873E-1145.0000043
AACGTAT253.8868307E-545.00000410
TTAGGCG253.8868307E-545.0000041
GGCAATT253.8868307E-545.0000048
TACGGGG253.8868307E-545.0000044
TTTCACG253.8868307E-545.0000041
ACCAGTT253.8868307E-545.00000412
GAACGTA253.8868307E-545.0000049
GGACGTC253.8868307E-545.0000048
TGCGTCG253.8868307E-545.0000041
TTTAGCG453.8380676E-1045.01
CGGGTGT207.028491E-445.06
ATGGGCG207.028491E-445.05
TGTCAAT207.028491E-445.041
CTGTCCG351.209628E-745.01
ACACGCG207.028491E-445.036
TACTTCG207.028491E-445.041
ACCATAT207.028491E-445.028
CGTGAGG207.028491E-445.02
TTCACGG351.209628E-745.02
Produced by FastQC (version 0.11.5)