##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549595_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	437535
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.351848423554685	33.0	31.0	34.0	31.0	34.0
2	32.56984241260699	34.0	31.0	34.0	31.0	34.0
3	32.62756579473642	34.0	31.0	34.0	31.0	34.0
4	36.026215045653494	37.0	35.0	37.0	35.0	37.0
5	36.07279874752877	37.0	35.0	37.0	35.0	37.0
6	35.662950392540026	37.0	35.0	37.0	35.0	37.0
7	36.01457483401328	37.0	35.0	37.0	35.0	37.0
8	36.02517741437828	37.0	35.0	37.0	35.0	37.0
9	37.85783537317015	39.0	38.0	39.0	35.0	39.0
10	37.55130675260265	39.0	37.0	39.0	35.0	39.0
11	37.51458054784189	39.0	37.0	39.0	35.0	39.0
12	37.289503696847106	39.0	37.0	39.0	35.0	39.0
13	37.13149119499011	39.0	37.0	39.0	33.0	39.0
14	38.2704880752397	40.0	38.0	41.0	34.0	41.0
15	38.37474259202121	40.0	38.0	41.0	34.0	41.0
16	38.47476430456992	40.0	38.0	41.0	34.0	41.0
17	38.4831224930577	40.0	38.0	41.0	35.0	41.0
18	38.43926314466271	40.0	37.0	41.0	35.0	41.0
19	38.46638097523627	40.0	37.0	41.0	35.0	41.0
20	38.378118321962816	40.0	37.0	41.0	34.0	41.0
21	38.29598546402002	40.0	37.0	41.0	34.0	41.0
22	38.276320751482736	40.0	37.0	41.0	34.0	41.0
23	38.21967156913161	40.0	37.0	41.0	34.0	41.0
24	38.20088678619996	40.0	37.0	41.0	34.0	41.0
25	38.10501331322066	40.0	37.0	41.0	34.0	41.0
26	38.06566788942599	40.0	37.0	41.0	34.0	41.0
27	38.06933388186088	40.0	36.0	41.0	34.0	41.0
28	37.97627389808815	40.0	36.0	41.0	34.0	41.0
29	37.94973887803261	40.0	36.0	41.0	34.0	41.0
30	37.92937250734227	40.0	36.0	41.0	34.0	41.0
31	37.84981544333596	40.0	36.0	41.0	34.0	41.0
32	37.744292456603475	40.0	36.0	41.0	33.0	41.0
33	37.685782851657585	40.0	36.0	41.0	33.0	41.0
34	37.70942895996892	40.0	36.0	41.0	33.0	41.0
35	37.6692607448547	40.0	36.0	41.0	33.0	41.0
36	37.59484841212703	40.0	36.0	41.0	33.0	41.0
37	37.47866570674346	40.0	35.0	41.0	33.0	41.0
38	37.386942758865004	40.0	35.0	41.0	33.0	41.0
39	37.36058601026203	39.0	35.0	41.0	33.0	41.0
40	37.309778646279725	39.0	35.0	41.0	33.0	41.0
41	37.24751162764122	39.0	35.0	41.0	33.0	41.0
42	37.2357388551773	39.0	35.0	41.0	33.0	41.0
43	37.116756373775814	39.0	35.0	41.0	32.0	41.0
44	36.96767572879884	39.0	35.0	41.0	32.0	41.0
45	36.97854800187414	39.0	35.0	41.0	32.0	41.0
46	36.897468773926654	39.0	35.0	41.0	32.0	41.0
47	36.820489789388276	39.0	35.0	41.0	32.0	41.0
48	36.76793627938336	39.0	35.0	41.0	32.0	41.0
49	36.804877324099785	39.0	35.0	41.0	32.0	41.0
50	36.7320511501937	39.0	35.0	41.0	32.0	41.0
51	35.709959203263736	38.0	34.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	12.0
11	17.0
12	6.0
13	3.0
14	6.0
15	10.0
16	15.0
17	26.0
18	48.0
19	100.0
20	153.0
21	311.0
22	508.0
23	725.0
24	1002.0
25	1575.0
26	2146.0
27	2570.0
28	3183.0
29	3773.0
30	4979.0
31	6628.0
32	8882.0
33	13085.0
34	25865.0
35	38600.0
36	31138.0
37	49438.0
38	97504.0
39	145161.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.41824082644817	4.31668323676963	56.731232929937036	15.533843006845165
2	20.537099889151726	5.472705040739598	51.890705886386236	22.099489183722447
3	20.463505776680723	5.1093055412709845	52.30461563074954	22.12257305129875
4	20.56909732935651	5.366656381775172	49.694767275760796	24.369479013107522
5	19.514324568320248	6.357662815546185	48.956997725896215	25.171014890237352
6	20.91969785274321	7.367639160295749	52.42871998811523	19.283942998845806
7	80.27792062349299	2.5986492509170693	11.096255156730319	6.027174968859634
8	80.2978047470488	3.78895402653502	9.07950221125167	6.833739015164501
9	73.8663192658873	6.294810700858217	12.476716148422412	7.362153884832071
10	37.58670734912636	28.111808198201288	19.855097306501193	14.446387146171164
11	29.59557521112597	25.54721336578788	25.391340121361722	19.465871301724434
12	27.03166603814552	21.377032694527294	31.716319837270163	19.874981430057023
13	23.724730592981132	25.5129303941399	32.12268732787091	18.639651685008058
14	19.171037745551782	29.459586090255634	29.96126024203778	21.4081159221548
15	15.95643777069263	25.73416983784154	36.996811683636736	21.31258070782909
16	18.723302135829133	25.392254333939	32.05412138457495	23.83032214565692
17	18.53154604774475	24.912064177722925	28.605482989932234	27.95090678460009
18	20.453906544619286	24.10458591884078	32.15445621493138	23.28705132160856
19	23.118150547956162	25.380826676723007	29.971088027243535	21.5299347480773
20	23.66736375375684	26.95304375649948	29.646542562309303	19.733049927434376
21	23.851349034934348	26.38531774600889	29.65088507205138	20.11244814700538
22	21.19076188190659	25.868559086701637	28.451209617516316	24.48946941387546
23	20.143531374632886	27.41723519261316	27.415178214314285	25.024055218439667
24	22.151599300627378	23.69296170592067	29.548264710251754	24.6071742832002
25	18.908887289016878	25.36939901950701	29.834641800084565	25.887071891391543
26	18.620453220885185	27.179768475664805	30.264550264550266	23.935228038899744
27	19.9977144685568	26.97772749608603	29.494783274480902	23.529774760876272
28	18.63508062212166	25.378769698424126	32.40289348280709	23.583256196647127
29	18.580913526917847	25.106334350394828	31.690950438250653	24.621801684436672
30	21.5207926223045	24.775160844275316	30.811706492052064	22.89234004136812
31	22.899882295130674	28.095352371810257	27.197595620921756	21.807169712137313
32	22.788348360702575	28.111808198201288	26.474910578582282	22.624932862513855
33	21.45794050761653	25.56984012707555	26.684036705634977	26.28818265967294
34	19.935548013301794	24.299084644657	30.06228073182717	25.703086610214037
35	19.558206772029667	23.541659524380908	29.618659078702276	27.281474624887153
36	22.359354108814152	22.86857051435885	29.70870901756431	25.06336635926269
37	21.049516038716902	25.369627572651332	30.01199904007679	23.56885734855497
38	21.73631823739815	28.437496428857116	26.840367056349777	22.98581827739495
39	20.42808003931114	25.178785697144228	31.30241009290685	23.090724170637777
40	22.726410458591882	22.073662678414298	32.39512267590021	22.804804187093605
41	19.88183802438662	22.53785411452798	29.70459506096655	27.875712800118848
42	22.569165895299808	22.033665878158317	27.20239523695247	28.194772989589406
43	22.249191493251967	21.54181951158193	28.590627035551442	27.618361959614656
44	20.885414881095226	22.997017381466627	29.17458031928874	26.942987418149407
45	20.301461597357925	23.131635183471037	28.647079662198454	27.919823556972585
46	22.61670494931834	22.277532083147634	32.01846709406105	23.08729587347298
47	19.385649148068154	22.573279851897563	33.29265087364439	24.748420126389888
48	19.908121635983406	21.482395694058763	32.10623150147988	26.50325116847795
49	21.423200429679913	19.363479493069125	34.143325676803	25.069994400447964
50	21.280354714479984	20.434022421063457	31.592444033048782	26.69317883140777
51	19.950861073971225	20.451621013176087	28.334418960768854	31.263098952083833
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	65.0
1	203.5
2	342.0
3	780.5
4	1219.0
5	908.5
6	598.0
7	607.5
8	617.0
9	776.5
10	936.0
11	931.0
12	926.0
13	900.0
14	874.0
15	847.0
16	820.0
17	842.0
18	864.0
19	841.0
20	818.0
21	1029.0
22	1240.0
23	1330.5
24	1421.0
25	1491.0
26	2069.0
27	2577.0
28	3150.5
29	3724.0
30	4491.5
31	5259.0
32	6079.0
33	6899.0
34	7943.0
35	8987.0
36	10163.5
37	11340.0
38	13304.0
39	15268.0
40	17419.5
41	19571.0
42	23987.0
43	28403.0
44	33049.0
45	37695.0
46	44128.5
47	50562.0
48	57521.0
49	64480.0
50	60687.5
51	56895.0
52	45219.5
53	33544.0
54	27945.5
55	22347.0
56	19713.5
57	17080.0
58	15084.5
59	13089.0
60	11289.0
61	9489.0
62	7896.5
63	6304.0
64	5385.0
65	4466.0
66	3714.5
67	2963.0
68	2425.0
69	1887.0
70	1471.0
71	1055.0
72	861.0
73	667.0
74	465.5
75	221.0
76	178.0
77	153.0
78	128.0
79	99.5
80	71.0
81	39.0
82	7.0
83	20.0
84	33.0
85	17.0
86	1.0
87	0.5
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	437535.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.644132883978
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.79512793084483	21.453180371299425
2	13.334542218350725	8.439200518090104
3	5.766694569685755	5.47446147793351
4	2.9549668728811826	3.740294575728203
5	1.7485382368581732	2.7665488159928318
6	1.203851289764575	2.285689811151511
7	0.7864604783201692	1.742080191877223
8	0.578411211574687	1.464265699252169
9	0.47960020684588467	1.365887940895306
>10	4.920153445707652	36.36965047236766
>50	0.35257751266362003	6.924921738132404
>100	0.07109587703916619	3.886128797716073
>500	0.0036273406652635805	0.8535341960012026
>1k	0.004352808798316296	3.234155393562384
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	3603	0.8234769789845383	TruSeq Adapter, Index 27 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGCT	3188	0.7286274240917869	Illumina Single End Adapter 2 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	2762	0.6312637846115168	TruSeq Adapter, Index 27 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTGC	2337	0.5341286982755665	TruSeq Adapter, Index 27 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	1106	0.2527797776177906	No Hit
TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC	1092	0.2495800335973122	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	886	0.2024980858674163	No Hit
GCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	792	0.18101409030134732	TruSeq Adapter, Index 27 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	720	0.16455826391031575	No Hit
TGCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	700	0.15998720102391809	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCT	620	0.14170294947832746	No Hit
TTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTCTG	473	0.10810563726330466	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGATTGCTCGTATGCCGTCTTC	454	0.10376312752122688	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7638246083170489	0.0
2	0.0	0.0	0.0	2.8991966356977157	0.0
3	0.0	0.0	0.0	4.172923308992424	0.0
4	0.0	0.0	0.0	5.432479687339298	0.0
5	0.0	0.0	0.0	7.370610351171906	0.0
6	0.0	0.0	0.0	8.595426651582159	0.0
7	0.0	0.0	0.0	9.674654599060647	0.0
8	0.0	0.0	0.0	11.439084873210144	0.0
9	0.0	0.0	0.0	12.308501034202978	0.0
10	0.0	0.0	0.0	13.902887768978482	0.0
11	0.0	0.0	0.0	16.701292468031127	0.0
12	0.0	0.0	0.0	19.336281668895058	0.0
13	0.0	0.0	0.0	20.192898853805982	0.0
14	0.0	0.0	0.0	20.49230347286503	0.0
15	0.0	0.0	0.0	20.946438570628636	0.0
16	0.0	0.0	0.0	22.046236301095913	0.0
17	0.0	0.0	0.0	23.67467745437508	0.0
18	0.0	0.0	0.0	25.54447072805604	0.0
19	0.0	0.0	0.0	26.673066154707623	0.0
20	0.0	0.0	0.0	27.9326225330545	0.0
21	0.0	0.0	0.0	29.458671877678356	0.0
22	0.0	0.0	0.0	31.024260916269554	0.0
23	0.0	0.0	0.0	32.32861371090313	0.0
24	0.0	0.0	0.0	33.316877506942305	0.0
25	0.0	0.0	0.0	34.25897356782886	0.0
26	0.0	0.0	0.0	35.071251442741726	0.0
27	0.0	0.0	0.0	35.90935582296274	0.0
28	0.0	0.0	0.0	36.70357799947433	0.0
29	0.0	0.0	0.0	37.56385203469437	0.0
30	0.0	0.0	0.0	38.541373832950505	0.0
31	0.0	0.0	0.0	39.46861393945627	0.0
32	2.2855314431988297E-4	0.0	0.0	40.29391934359537	0.0
33	2.2855314431988297E-4	0.0	0.0	41.0885986263956	0.0
34	4.5710628863976594E-4	0.0	0.0	41.83779583347618	0.0
35	4.5710628863976594E-4	0.0	0.0	42.68344246745975	0.0
36	4.5710628863976594E-4	0.0	0.0	43.44680996948816	0.0
37	4.5710628863976594E-4	0.0	0.0	44.2186339378564	0.0
38	4.5710628863976594E-4	0.0	0.0	44.96394574148354	0.0
39	4.5710628863976594E-4	0.0	0.0	45.77942336041688	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGG	50	2.1827873E-11	45.000004	3
AACGTAT	25	3.8868307E-5	45.000004	10
TTAGGCG	25	3.8868307E-5	45.000004	1
GGCAATT	25	3.8868307E-5	45.000004	8
TACGGGG	25	3.8868307E-5	45.000004	4
TTTCACG	25	3.8868307E-5	45.000004	1
ACCAGTT	25	3.8868307E-5	45.000004	12
GAACGTA	25	3.8868307E-5	45.000004	9
GGACGTC	25	3.8868307E-5	45.000004	8
TGCGTCG	25	3.8868307E-5	45.000004	1
TTTAGCG	45	3.8380676E-10	45.0	1
CGGGTGT	20	7.028491E-4	45.0	6
ATGGGCG	20	7.028491E-4	45.0	5
TGTCAAT	20	7.028491E-4	45.0	41
CTGTCCG	35	1.209628E-7	45.0	1
ACACGCG	20	7.028491E-4	45.0	36
TACTTCG	20	7.028491E-4	45.0	41
ACCATAT	20	7.028491E-4	45.0	28
CGTGAGG	20	7.028491E-4	45.0	2
TTCACGG	35	1.209628E-7	45.0	2
>>END_MODULE