SRR3549592_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549592_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	99388
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGCT	584	0.5875960880589206	RNA PCR Primer, Index 21 (96% over 27bp)
TCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	571	0.5745160381534995	TruSeq Adapter, Index 21 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	508	0.5111281039964584	RNA PCR Primer, Index 21 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	491	0.4940234233509076	TruSeq Adapter, Index 21 (96% over 25bp)
CGGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCT	352	0.35416750513140416	RNA PCR Primer, Index 21 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	313	0.31492735541514066	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	235	0.2364470559826136	RNA PCR Primer, Index 21 (96% over 25bp)
TCCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	193	0.19418843321125287	RNA PCR Primer, Index 21 (96% over 25bp)
TTCCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCT	161	0.16199138729021614	TruSeq Adapter, Index 21 (95% over 23bp)
TGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	146	0.14689902201473015	RNA PCR Primer, Index 14 (95% over 24bp)
CGTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	135	0.13583128747937376	RNA PCR Primer, Index 14 (95% over 22bp)
CGGTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	118	0.118726606833823	RNA PCR Primer, Index 21 (95% over 23bp)
GCCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	111	0.1116835030385962	TruSeq Adapter, Index 21 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCC	110	0.11067734535356383	No Hit
TTTCCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	100	0.10061576850323983	RNA PCR Primer, Index 14 (95% over 22bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTTCGG	30	2.145467E-6	45.000004	2
TACGGGA	30	2.145467E-6	45.000004	4
CGGGAAC	60	0.0	45.000004	6
TTTGCCG	30	2.145467E-6	45.000004	1
GGAGACC	20	7.0021796E-4	45.0	8
TTGCTCG	20	7.0021796E-4	45.0	1
TTTCGCG	20	7.0021796E-4	45.0	1
CAACAAT	20	7.0021796E-4	45.0	26
TGACGGG	20	7.0021796E-4	45.0	3
ACGGGAT	20	7.0021796E-4	45.0	5
CGGGATA	45	3.765308E-10	45.0	6
GCGATTC	35	1.1970042E-7	45.0	9
CGGGACT	40	6.706614E-9	45.0	6
GACGGGA	20	7.0021796E-4	45.0	4
GCGATGC	20	7.0021796E-4	45.0	9
ACGTCTC	20	7.0021796E-4	45.0	34
TCTTTCG	25	3.865044E-5	45.0	1
TTGCCGG	35	1.1970042E-7	45.0	2
GAATTGA	20	7.0021796E-4	45.0	9
GGCGATG	40	6.706614E-9	45.0	8

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content