FastQCFastQC Report
Sat 18 Jun 2016
SRR3549591_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549591_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1642776
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39790.24221196316478935No Hit
CCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC38170.23235060653430534No Hit
CTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGCT37630.22906348765747733No Hit
GCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC32800.19966203548140463No Hit
TCTGTCTCTTATACACATCTGACGCCAGAGCATTCGTATGCCGTCTTCTGC29640.18042630279478153No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGATAT551.8189894E-1245.09
TAAGCGC207.0341723E-445.034
TACACCG207.0341723E-445.041
TTTTGCG14450.039.7058831
TTGGGAC37500.039.4799965
CGTTTGG9300.039.4354862
TACGGGT403.4594268E-739.3754
TCGACGT403.4594268E-739.37526
CGTTTTT21050.039.3349151
ACACGCG3100.039.19354636
TTACACG3150.038.5714334
TTTGGGA99450.038.371044
GACCGAT6400.038.3203129
TGGGCGA18050.038.019396
TTTCGCG6100.037.9918021
TAACGAC301.1401356E-437.49999628
TATCTCG301.1401356E-437.4999961
CGCAACA301.1401356E-437.49999623
CACGCGG3200.037.26562537
TTGGGAT63500.037.1692925