SRR3549590_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549590_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	926985
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	5578	0.6017357346666883	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGCT	4675	0.5043231551751107	TruSeq Adapter, Index 21 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	3156	0.34045858347222446	TruSeq Adapter, Index 15 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	2686	0.2897565764278818	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1281	0.13818993834851695	No Hit
CGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG	1173	0.1265392643893914	TruSeq Adapter, Index 21 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG	1053	0.11359407110147414	TruSeq Adapter, Index 21 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG	931	0.10043312459209155	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	20	7.032578E-4	45.000004	8
CGCCTAT	20	7.032578E-4	45.000004	10
ATATCGT	20	7.032578E-4	45.000004	25
TCGGAAT	20	7.032578E-4	45.000004	5
ACTCGAT	20	7.032578E-4	45.000004	38
CTCGATA	20	7.032578E-4	45.000004	39
CTTTCGT	20	7.032578E-4	45.000004	15
CGAACGA	35	1.2115925E-7	45.0	22
CGTATGG	25	3.8902195E-5	45.0	2
TCCGATA	35	1.2115925E-7	45.0	14
TCTACCG	35	1.2115925E-7	45.0	1
GGCGATA	25	3.8902195E-5	45.0	8
CGATAAC	35	1.2115925E-7	45.0	16
CCGTTCG	25	3.8902195E-5	45.0	1
CCGTTAG	25	3.8902195E-5	45.0	1
AGGCGAA	30	2.1650467E-6	44.999996	18
CTACGAA	60	0.0	44.999996	11
TTTGTCG	200	0.0	43.875	1
GCGCGAC	145	0.0	41.896553	9
GTGCCTA	65	0.0	41.53846	1