##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549590_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	926985
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.4385335253537	33.0	31.0	34.0	31.0	34.0
2	32.660969702853876	34.0	31.0	34.0	31.0	34.0
3	32.71959632572264	34.0	31.0	34.0	31.0	34.0
4	36.08885364919605	37.0	35.0	37.0	35.0	37.0
5	36.12486286185861	37.0	35.0	37.0	35.0	37.0
6	35.73593208088588	37.0	35.0	37.0	35.0	37.0
7	36.04511723490671	37.0	35.0	37.0	35.0	37.0
8	36.05832025329428	37.0	36.0	37.0	35.0	37.0
9	37.908974794629906	39.0	38.0	39.0	35.0	39.0
10	37.60193530639654	39.0	37.0	39.0	35.0	39.0
11	37.562352141620416	39.0	37.0	39.0	35.0	39.0
12	37.389593143362625	39.0	37.0	39.0	35.0	39.0
13	37.33181335188811	39.0	37.0	39.0	34.0	39.0
14	38.55201756231223	40.0	38.0	41.0	35.0	41.0
15	38.62599610565435	40.0	38.0	41.0	35.0	41.0
16	38.683358414645326	40.0	38.0	41.0	35.0	41.0
17	38.655446420384365	40.0	38.0	41.0	35.0	41.0
18	38.615673392773346	40.0	38.0	41.0	35.0	41.0
19	38.63552484668037	40.0	38.0	41.0	35.0	41.0
20	38.573663004255735	40.0	38.0	41.0	35.0	41.0
21	38.50164350016451	40.0	38.0	41.0	34.0	41.0
22	38.478505045928465	40.0	38.0	41.0	34.0	41.0
23	38.43909772002783	40.0	38.0	41.0	34.0	41.0
24	38.418364914211125	40.0	37.0	41.0	34.0	41.0
25	38.315643726705396	40.0	37.0	41.0	34.0	41.0
26	38.248972744974296	40.0	37.0	41.0	34.0	41.0
27	38.21196783119468	40.0	37.0	41.0	34.0	41.0
28	38.15338220143799	40.0	37.0	41.0	34.0	41.0
29	38.151386484139444	40.0	37.0	41.0	34.0	41.0
30	38.08500784802343	40.0	37.0	41.0	34.0	41.0
31	38.004880337869544	40.0	37.0	41.0	34.0	41.0
32	37.85102671564265	40.0	36.0	41.0	33.0	41.0
33	37.785872479058455	40.0	36.0	41.0	33.0	41.0
34	37.77965447121582	40.0	36.0	41.0	33.0	41.0
35	37.659872597722725	40.0	36.0	41.0	33.0	41.0
36	37.57178487246288	40.0	36.0	41.0	33.0	41.0
37	37.52364924998786	40.0	36.0	41.0	33.0	41.0
38	37.47081128605102	40.0	35.0	41.0	33.0	41.0
39	37.47371100934751	40.0	36.0	41.0	33.0	41.0
40	37.367884054218784	40.0	35.0	41.0	33.0	41.0
41	37.17853363323031	39.0	35.0	41.0	32.0	41.0
42	37.0809398210327	39.0	35.0	41.0	32.0	41.0
43	37.03268661305199	39.0	35.0	41.0	32.0	41.0
44	36.987573693209704	39.0	35.0	41.0	32.0	41.0
45	37.010513654482004	39.0	35.0	41.0	32.0	41.0
46	36.89643953246277	39.0	35.0	41.0	32.0	41.0
47	36.78442909000685	39.0	35.0	41.0	31.0	41.0
48	36.69845358878515	39.0	35.0	41.0	31.0	41.0
49	36.689651936115474	39.0	35.0	41.0	31.0	41.0
50	36.61247916632955	39.0	35.0	40.0	31.0	41.0
51	35.567720081770474	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	6.0
9	23.0
10	18.0
11	23.0
12	17.0
13	12.0
14	13.0
15	26.0
16	40.0
17	61.0
18	103.0
19	186.0
20	383.0
21	634.0
22	930.0
23	1425.0
24	2060.0
25	3071.0
26	4419.0
27	5523.0
28	6637.0
29	8172.0
30	10346.0
31	13408.0
32	18161.0
33	25966.0
34	51590.0
35	72208.0
36	66409.0
37	109140.0
38	209723.0
39	316055.0
40	197.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.65575494749106	4.890370394342951	52.7648235947723	16.68905106339369
2	20.090076969961757	4.674617172877663	52.32889421080168	22.906411646358897
3	21.28966488130876	4.695760988581261	51.44613990517646	22.56843422493352
4	19.728906077228867	5.100945538493072	49.722055912447345	25.44809247183072
5	19.0406533007546	6.348322788394634	48.8947501847387	25.71627372611207
6	22.416436080411227	7.070556697249685	51.58422196691424	18.928785255424845
7	82.3347734860866	3.3822553763005874	9.219890289486884	5.063080848125914
8	83.21601751916158	3.6428852678306556	7.612744542791955	5.528352670215807
9	77.20211222403815	5.91854237123578	10.422714499155864	6.456630905570209
10	37.65519398911525	27.966471949384292	18.980997535019444	15.397336526481013
11	29.171453691267928	25.268801544793067	25.233310139862024	20.32643462407698
12	27.056748491075904	21.786113043900386	29.855930786366553	21.301207678657153
13	24.9605980679299	23.521200450924233	30.753895694105083	20.764305787040783
14	21.06118221977702	26.105168907803254	29.96520979303872	22.868439079381
15	18.863088399488664	25.159954044563825	33.92654681575214	22.050410740195364
16	20.819970118178826	24.303413755346632	31.996418496523678	22.880197629950864
17	20.90174058911417	24.924027896891534	29.439958575381482	24.734272938612815
18	21.409407919221994	24.70708803270819	30.85745724040842	23.026046807661395
19	23.28937361445978	25.710772018964708	29.21244680334633	21.787407563229177
20	25.303753566670444	26.372271395977283	29.138335571773005	19.18563946557927
21	25.086921579097826	26.6198482176087	27.99764828988603	20.295581913407446
22	22.701122456134673	25.594373155984186	28.25331585732239	23.45118853055875
23	22.110713765594912	25.728571659735593	28.08254718253262	24.078167392136873
24	22.532403436948815	24.241924087229027	29.67717924238256	23.54849323343959
25	21.70660798179043	26.005922425929224	27.45243989924325	24.835029693037104
26	21.31911519603877	27.520941547058474	27.42870704488206	23.73123621202069
27	21.591395761527966	27.600554485779167	28.046947900990848	22.761101851702023
28	18.416910737498448	26.621142736937493	30.026915214377798	24.935031311186265
29	20.68382983543423	25.51281843827031	29.333052854145432	24.470298872150035
30	23.07545429537695	26.151447973807556	27.868951493281983	22.90414623753351
31	23.41062692492327	27.41662486448001	26.003549140493103	23.169199070103616
32	23.295198951439346	27.988586654584484	25.879059531707632	22.837154862268537
33	23.164020992788448	27.61781474349639	26.06956962626148	23.14859463745368
34	22.14814694951914	25.423604481194413	27.90185385955544	24.526394709731008
35	22.19410238569125	25.85047222986348	27.626552748965732	24.32887263547954
36	23.57913019088766	27.99743253666456	25.987799155326137	22.435638117121638
37	21.410486685329317	29.22075330237275	27.087709078356177	22.281050933941756
38	20.504431031785845	28.594421700459016	26.79568709310291	24.105460174652233
39	23.07890634692039	27.386095783642673	27.202705545397176	22.332292324039763
40	25.339784354655144	24.698134274017377	28.083625948639945	21.87845542268753
41	21.71070729299827	25.355210709989912	27.696133162888287	25.23794883412353
42	21.486971202338765	26.293089963699522	27.382212225656293	24.83772660830542
43	21.303688840704	26.43063264238364	27.365599227603465	24.900079289308888
44	22.189895197872673	25.003425082390763	26.940025998263184	25.866653721473376
45	23.32982734348452	24.325420583936094	25.79232673667859	26.552425335900796
46	23.766619740340996	25.67743814624832	27.539064817661558	23.016877295749122
47	20.20226864512371	26.143680857834813	29.52216055275975	24.13188994428173
48	20.279184668576082	25.386494927102383	29.791959956202096	24.542360448119442
49	22.59335372201276	23.939545947345426	30.175892813799578	23.291207516842235
50	22.75527651472246	23.461544685189082	28.17488956131976	25.608289238768695
51	20.603353883827676	23.61321919987918	26.861599702260552	28.921827214032593
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	177.0
1	562.5
2	948.0
3	1721.0
4	2494.0
5	1781.0
6	1068.0
7	1065.5
8	1063.0
9	1194.5
10	1326.0
11	1375.5
12	1425.0
13	1466.0
14	1507.0
15	1570.0
16	1633.0
17	1713.5
18	1794.0
19	1895.5
20	1997.0
21	2314.0
22	2631.0
23	2887.0
24	3143.0
25	4360.5
26	6882.0
27	8186.0
28	9147.0
29	10108.0
30	11949.0
31	13790.0
32	15634.0
33	17478.0
34	20001.5
35	22525.0
36	25029.5
37	27534.0
38	30355.5
39	33177.0
40	39025.0
41	44873.0
42	49262.0
43	53651.0
44	60600.0
45	67549.0
46	75874.5
47	84200.0
48	96350.0
49	108500.0
50	105958.0
51	103416.0
52	88588.5
53	73761.0
54	64157.0
55	54553.0
56	49372.0
57	44191.0
58	39518.0
59	34845.0
60	33177.0
61	31509.0
62	27211.5
63	22914.0
64	19156.5
65	15399.0
66	12423.0
67	9447.0
68	8414.0
69	7381.0
70	5945.5
71	4510.0
72	3781.0
73	3052.0
74	2359.5
75	1376.5
76	1086.0
77	792.5
78	499.0
79	359.0
80	219.0
81	160.5
82	102.0
83	83.5
84	65.0
85	36.5
86	8.0
87	5.5
88	3.0
89	2.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	926985.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.41804037710815
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.290948705869	19.406949377144066
2	12.805055470941722	7.277891668086631
3	5.3164988145192575	4.532534339275676
4	2.6614098986194086	3.0252821583600666
5	1.6147948667562522	2.2944652862113073
6	1.018039141778573	1.735840646192401
7	0.7353914453417795	1.4628868650691824
8	0.5636225466602062	1.2813638630750608
9	0.4344403803273145	1.111134984262906
>10	5.952706214887183	42.60836953176608
>50	0.5287580017356595	9.11975407553762
>100	0.07295861464259747	3.5077653600479124
>500	0.0026879489605167307	0.496728820001183
>1k	0.0023039562518714835	1.5303437987031174
>5k	3.839927086452472E-4	0.6086892262668275
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	5578	0.6017357346666883	TruSeq Adapter, Index 15 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGCT	4675	0.5043231551751107	TruSeq Adapter, Index 21 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	3156	0.34045858347222446	TruSeq Adapter, Index 15 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTGC	2686	0.2897565764278818	TruSeq Adapter, Index 15 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1281	0.13818993834851695	No Hit
CGCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG	1173	0.1265392643893914	TruSeq Adapter, Index 21 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG	1053	0.11359407110147414	TruSeq Adapter, Index 21 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCGACAGGTATCGTATGCCGTCTTCTG	931	0.10043312459209155	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5321553207441329	0.0
2	0.0	0.0	0.0	1.8702568002718492	0.0
3	0.0	0.0	0.0	2.575985587684806	0.0
4	0.0	0.0	0.0	3.2958462111037394	0.0
5	0.0	0.0	0.0	4.5201378663085165	0.0
6	0.0	0.0	0.0	5.233202263251293	0.0
7	0.0	0.0	0.0	5.946913919858466	0.0
8	0.0	0.0	0.0	7.107342621509518	0.0
9	0.0	0.0	0.0	7.630328430341375	0.0
10	0.0	0.0	0.0	8.690000377568138	0.0
11	0.0	0.0	0.0	10.711068679644223	0.0
12	0.0	0.0	0.0	12.63914734326877	0.0
13	0.0	0.0	0.0	13.267312847564956	0.0
14	0.0	0.0	0.0	13.484792094801966	0.0
15	0.0	0.0	0.0	13.898067390518724	0.0
16	0.0	0.0	0.0	14.712535801550187	0.0
17	0.0	0.0	0.0	15.79389094753421	0.0
18	0.0	0.0	0.0	16.936951514857306	0.0
19	0.0	0.0	0.0	17.756921633036132	0.0
20	0.0	0.0	0.0	18.672686181545547	0.0
21	0.0	0.0	0.0	19.926859657923266	0.0
22	0.0	0.0	0.0	21.206707767655356	0.0
23	0.0	0.0	0.0	22.34437450444182	0.0
24	0.0	0.0	0.0	23.255931865132663	0.0
25	0.0	0.0	0.0	24.07676499619735	0.0
26	0.0	0.0	0.0	24.787671860925474	0.0
27	0.0	0.0	0.0	25.488761954076928	0.0
28	0.0	0.0	0.0	26.24120131393712	0.0
29	0.0	0.0	0.0	26.987275953764083	0.0
30	0.0	0.0	0.0	27.836372756840724	0.0
31	0.0	0.0	0.0	28.599276147941985	0.0
32	0.0	0.0	0.0	29.334563126695684	0.0
33	0.0	0.0	0.0	30.037487122229592	0.0
34	0.0	0.0	0.0	30.76845903655399	0.0
35	0.0	0.0	0.0	31.537295641245542	0.0
36	0.0	0.0	0.0	32.25036003818832	0.0
37	0.0	0.0	0.0	32.93688678889087	0.0
38	0.0	0.0	0.0	33.64002653764624	0.0
39	0.0	0.0	0.0	34.38469878153368	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	20	7.032578E-4	45.000004	8
CGCCTAT	20	7.032578E-4	45.000004	10
ATATCGT	20	7.032578E-4	45.000004	25
TCGGAAT	20	7.032578E-4	45.000004	5
ACTCGAT	20	7.032578E-4	45.000004	38
CTCGATA	20	7.032578E-4	45.000004	39
CTTTCGT	20	7.032578E-4	45.000004	15
CGAACGA	35	1.2115925E-7	45.0	22
CGTATGG	25	3.8902195E-5	45.0	2
TCCGATA	35	1.2115925E-7	45.0	14
TCTACCG	35	1.2115925E-7	45.0	1
GGCGATA	25	3.8902195E-5	45.0	8
CGATAAC	35	1.2115925E-7	45.0	16
CCGTTCG	25	3.8902195E-5	45.0	1
CCGTTAG	25	3.8902195E-5	45.0	1
AGGCGAA	30	2.1650467E-6	44.999996	18
CTACGAA	60	0.0	44.999996	11
TTTGTCG	200	0.0	43.875	1
GCGCGAC	145	0.0	41.896553	9
GTGCCTA	65	0.0	41.53846	1
>>END_MODULE