FastQCFastQC Report
Sat 18 Jun 2016
SRR3549587_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549587_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431504
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGC256185.93690904371686No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCG219935.096824131410138No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTC173174.01317253142497No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGTTCTCT88782.0574548555749193No Hit
GCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC80151.8574567095554155Illumina PCR Primer Index 10 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTA54621.265805183729467No Hit
GAACTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCT46241.0716007267603544Illumina PCR Primer Index 10 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTC40300.9339426749230597No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCT39020.9042789869850569No Hit
CTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGCT28850.6685917164151434Illumina PCR Primer Index 10 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC27490.6370740479810152Illumina PCR Primer Index 10 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC21130.4896825985390633Illumina PCR Primer Index 10 (95% over 23bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGT19150.44379658125996513No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATG16190.3751993029033335No Hit
TGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGCTT14220.3295450331862509Illumina PCR Primer Index 10 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11860.27485260855055804No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTAT11720.271608142682339No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTT11640.2697541621862138No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCGTTCTC11110.2574715413993845No Hit
CGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG8350.19350921428306575Illumina PCR Primer Index 10 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG5230.1212039749341837Illumina PCR Primer Index 10 (95% over 22bp)
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCTCGTTCTCTCG5150.11934999443805851No Hit
TGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG5070.11749601394193333Illumina PCR Primer Index 10 (95% over 22bp)
GAATGCTACGGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGC4680.10845785902332307No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGC4430.10266416997293187No Hit
TTCCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCT4420.10243242241091623Illumina PCR Primer Index 10 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTACGG351.2095734E-745.0000042
ATACTCG351.2095734E-745.00000444
TCGCCGA207.028385E-445.044
TGCCCGA253.886743E-545.039
ATAGGGT406.7975634E-945.024
CACGGCG253.886743E-545.021
GAATGCA207.028385E-445.09
ACTGACG406.7975634E-945.043
TTAGCCG207.028385E-445.025
TAGACCA253.886743E-545.024
GCGAACC253.886743E-545.033
TTTCGCG950.045.01
GGACTAA800.045.08
GGCCGAT502.1827873E-1145.08
ATGTCGT207.028385E-445.038
TGTGCCG207.028385E-445.01
TTGAGCG207.028385E-445.015
GTGCTTA207.028385E-445.021
CACTACT253.886743E-545.013
ACTACGG207.028385E-445.025
Produced by FastQC (version 0.11.5)