##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549587_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	431504
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.055777003225927	28.0	16.0	31.0	16.0	34.0
2	25.9594024620861	30.0	16.0	33.0	16.0	34.0
3	27.078685249731173	31.0	23.0	33.0	16.0	34.0
4	31.25665810745671	33.0	28.0	35.0	19.0	37.0
5	33.256794838518296	35.0	32.0	35.0	28.0	37.0
6	31.57834921576625	35.0	28.0	37.0	17.0	37.0
7	33.10929910267344	35.0	32.0	35.0	28.0	37.0
8	32.075561292595204	35.0	32.0	37.0	17.0	37.0
9	34.21359709295858	35.0	32.0	38.0	28.0	39.0
10	33.53380733434684	35.0	32.0	37.0	27.0	39.0
11	34.13497209759353	35.0	32.0	39.0	27.0	39.0
12	34.42342133560755	37.0	33.0	39.0	27.0	39.0
13	34.59398522377545	35.0	33.0	38.0	27.0	39.0
14	34.42786625384701	36.0	32.0	39.0	27.0	40.0
15	35.055739923616	37.0	33.0	39.0	27.0	40.0
16	34.71755534131781	36.0	32.0	39.0	27.0	40.0
17	34.15225119581742	36.0	32.0	38.0	27.0	40.0
18	34.36448329563573	36.0	32.0	39.0	27.0	40.0
19	34.153011327820835	36.0	32.0	39.0	27.0	40.0
20	34.07103294523342	36.0	32.0	39.0	25.0	40.0
21	33.98041037858282	36.0	32.0	39.0	25.0	40.0
22	35.114001260706736	37.0	33.0	39.0	27.0	40.0
23	35.48313804738774	37.0	34.0	39.0	29.0	40.0
24	35.76103118395194	38.0	34.0	39.0	30.0	40.0
25	33.80561477993251	36.0	32.0	39.0	24.0	40.0
26	34.58106993214432	37.0	33.0	39.0	27.0	40.0
27	34.81262514368349	37.0	33.0	39.0	27.0	40.0
28	34.981423115428825	37.0	33.0	39.0	27.0	40.0
29	35.34251594423227	38.0	34.0	40.0	27.0	40.0
30	33.71972913344952	36.0	32.0	39.0	24.0	40.0
31	34.15575521895509	36.0	32.0	39.0	25.0	40.0
32	34.61249026660239	37.0	33.0	39.0	26.0	40.0
33	35.083494011643	37.0	34.0	39.0	27.0	40.0
34	35.262396177092214	38.0	34.0	40.0	27.0	40.0
35	35.44007703288961	38.0	34.0	40.0	27.0	41.0
36	35.33028894286032	38.0	34.0	40.0	26.0	41.0
37	35.665595220438284	38.0	35.0	40.0	30.0	41.0
38	35.16929854647929	37.0	34.0	40.0	28.0	41.0
39	34.91238783417998	37.0	33.0	40.0	26.0	41.0
40	34.748873706848606	37.0	33.0	40.0	26.0	41.0
41	34.05621500611814	36.0	32.0	39.0	24.0	40.0
42	34.99847510104194	37.0	34.0	40.0	28.0	40.0
43	35.17761596648003	38.0	34.0	40.0	28.0	40.0
44	35.74772887389225	38.0	35.0	40.0	30.0	41.0
45	36.07845581964478	38.0	35.0	40.0	31.0	41.0
46	35.7328228707034	38.0	35.0	40.0	30.0	41.0
47	35.275253531832846	38.0	34.0	40.0	26.0	41.0
48	35.41958823093181	38.0	34.0	40.0	28.0	41.0
49	35.80698672549965	38.0	35.0	40.0	30.0	41.0
50	35.47779626608328	38.0	35.0	40.0	28.0	41.0
51	34.949761763506245	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	1.0
10	8.0
11	9.0
12	17.0
13	15.0
14	25.0
15	34.0
16	70.0
17	216.0
18	657.0
19	1471.0
20	2114.0
21	2607.0
22	2774.0
23	2803.0
24	3174.0
25	4158.0
26	5702.0
27	7469.0
28	9616.0
29	12709.0
30	17123.0
31	22867.0
32	31077.0
33	39570.0
34	47895.0
35	57040.0
36	63546.0
37	58855.0
38	34300.0
39	3580.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.92796358782306	3.1575605324631986	37.10904186287961	13.805434016834143
2	16.324066520820203	31.41755348733731	34.93223701286662	17.326142978975863
3	15.118283955652787	31.188355148503838	38.079137157477106	15.614223738366274
4	16.027893136564202	3.1570970373391667	61.87984352404613	18.9351663020505
5	36.61240683748007	4.1005413623048685	36.693750231747565	22.5933015684675
6	16.75233601542512	26.999286217508995	40.10345211168379	16.144925655382107
7	58.11325039860581	1.7608179761948906	34.7556453706107	5.370286254588601
8	53.89057807111869	22.626904964959767	13.939384107679187	9.543132856242352
9	50.241712707182316	4.173773591901813	14.344247098520524	31.240266602395344
10	39.80496125180763	21.558085208943602	21.656114427676222	16.980839111572546
11	33.1561236975787	20.719854277133003	27.23566873076495	18.88835329452334
12	23.876719566910158	15.889308094478846	30.492880714894877	29.74109162371612
13	24.566863806592753	19.99494790314806	39.65478883162149	15.783399458637696
14	19.1513867774111	30.349197226445177	24.57358448589121	25.92583151025251
15	11.586451110534316	23.45123104304943	42.956264600096404	22.006053246319848
16	13.191302977492677	24.512634877081094	25.416218621380104	36.87984352404613
17	14.431384181838409	29.157551262560716	34.02610404538544	22.384960510215432
18	12.574854462531054	24.564082835848566	32.857632837702546	30.00342986391783
19	14.80125329081538	27.22524009047425	28.08456005042827	29.888946568282105
20	19.653351996736994	24.996523786569767	40.122918906893105	15.22720530980014
21	21.575929771218807	33.61985056917201	26.72883681263673	18.07538284697245
22	15.11341725685046	21.798407430753826	34.33108383699803	28.75709147539768
23	18.195428084096555	32.30097519374096	28.033343839222812	21.470252882939672
24	21.78288034409878	23.146019503874818	25.62015647595387	29.450943676072527
25	14.3690440876562	37.119933998294336	22.63687011012644	25.874151803923024
26	16.213986428862768	22.03061848789351	33.252530683377216	28.50286439986651
27	21.509881716044347	27.84493307130409	23.907078497534208	26.738106715117354
28	15.372279283621937	25.695706181170973	35.95748822722385	22.97452630798324
29	20.034576736252735	17.786857132262966	34.37233490303682	27.806231228447476
30	17.397752975638696	27.631493566687677	31.197856798546482	23.772896659127145
31	24.868367384775112	19.924960139419333	24.548324001631503	30.65834847417405
32	21.987513441358598	27.002298935815194	31.306082910007788	19.70410471281842
33	17.486744039452706	17.71802810634432	27.016667284660166	37.778560569542805
34	22.7902869961808	18.971318921724944	32.267140049686674	25.971254032407582
35	15.661036745893433	17.29323482516964	32.55079906559383	34.4949293633431
36	21.450090845044308	19.82090548407431	30.189523156216396	28.539480514664984
37	15.53334383922281	18.34096555304238	37.83394823686455	28.29174237087026
38	25.309382995290893	19.344432496570136	29.566585709518336	25.77959879862064
39	19.2195205606437	20.05612925952019	34.997358077793024	25.726992102043088
40	17.177824539285847	17.81443509214283	41.05106789276577	23.956672475805554
41	17.985001297786347	21.407912788757464	23.842884422855875	36.76420149060032
42	17.66982461344507	16.96206755904928	40.16996366198228	25.198144165523377
43	21.268864251548074	17.878165671697133	27.28456746635025	33.56840261040454
44	20.173393525900106	16.9386610552857	32.56980236567911	30.31814305313508
45	20.422753902628944	14.611683785086582	30.20528199043346	34.76028032185101
46	27.14366494864474	22.263524787719234	29.02174719121955	21.57106307241648
47	13.625366161147983	17.673996069561348	46.661444621602584	22.039193147688085
48	20.585208943601913	21.252641922206976	27.124893396121475	31.037255738069636
49	18.023239645518927	14.59847417405169	42.99403945270496	24.384246727724424
50	22.910332233304906	15.389196855649079	31.211298157143386	30.489172753902626
51	18.653361266639475	15.50298490859876	29.018734472913344	36.824919351848415
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	62.0
1	226.0
2	390.0
3	1283.5
4	2177.0
5	1541.5
6	906.0
7	998.5
8	1091.0
9	1281.5
10	1472.0
11	1490.5
12	1509.0
13	1496.5
14	1484.0
15	1363.5
16	1243.0
17	1144.5
18	1046.0
19	1142.5
20	1239.0
21	1297.0
22	1355.0
23	1519.5
24	1684.0
25	1953.5
26	2415.0
27	2607.0
28	3272.5
29	3938.0
30	4301.0
31	4664.0
32	5498.5
33	6333.0
34	6747.5
35	7162.0
36	7561.0
37	7960.0
38	9147.5
39	10335.0
40	11623.5
41	12912.0
42	14192.0
43	15472.0
44	18387.0
45	21302.0
46	69401.5
47	117501.0
48	87830.5
49	58160.0
50	52433.0
51	46706.0
52	35761.0
53	24816.0
54	20916.0
55	17016.0
56	15450.0
57	13884.0
58	12949.5
59	12015.0
60	11201.0
61	10387.0
62	8875.0
63	7363.0
64	5881.5
65	4400.0
66	3562.5
67	2725.0
68	2458.5
69	2192.0
70	1788.5
71	1385.0
72	1267.0
73	1149.0
74	857.0
75	451.5
76	338.0
77	225.5
78	113.0
79	110.5
80	108.0
81	79.0
82	50.0
83	35.0
84	20.0
85	16.5
86	13.0
87	22.0
88	31.0
89	16.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	431504.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.06809392824934
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.11974189331369	21.084679713273754
2	10.297951350547295	5.780877315514072
3	3.729294212466341	3.140225407247457
4	1.873881644233442	2.1038514400306627
5	1.122145442028821	1.5748241834010912
6	0.779630548939525	1.3129646086180309
7	0.6118619148477319	1.2021658387945247
8	0.5205294497366122	1.1688215590101738
9	0.439168971581895	1.1093972350259964
>10	5.313191389216058	27.89614731251421
>50	0.1037732667503118	2.0095422634645566
>100	0.06973563525620954	3.758105012193457
>500	0.003320744536009978	0.5545814688132705
>1k	0.010792419742032428	6.965356834355581
>5k	0.002490558402007483	5.209104510638934
>10k+	0.002490558402007483	15.129355297104214
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGC	25618	5.93690904371686	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCG	21993	5.096824131410138	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTC	17317	4.01317253142497	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCGTTCTCT	8878	2.0574548555749193	No Hit
GCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC	8015	1.8574567095554155	Illumina PCR Primer Index 10 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTA	5462	1.265805183729467	No Hit
GAACTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCT	4624	1.0716007267603544	Illumina PCR Primer Index 10 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTC	4030	0.9339426749230597	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCT	3902	0.9042789869850569	No Hit
CTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGCT	2885	0.6685917164151434	Illumina PCR Primer Index 10 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC	2749	0.6370740479810152	Illumina PCR Primer Index 10 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC	2113	0.4896825985390633	Illumina PCR Primer Index 10 (95% over 23bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGT	1915	0.44379658125996513	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATG	1619	0.3751993029033335	No Hit
TGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGCTT	1422	0.3295450331862509	Illumina PCR Primer Index 10 (96% over 25bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1186	0.27485260855055804	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTAT	1172	0.271608142682339	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTT	1164	0.2697541621862138	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCGTTCTC	1111	0.2574715413993845	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG	835	0.19350921428306575	Illumina PCR Primer Index 10 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG	523	0.1212039749341837	Illumina PCR Primer Index 10 (95% over 22bp)
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCTCGTTCTCTCG	515	0.11934999443805851	No Hit
TGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG	507	0.11749601394193333	Illumina PCR Primer Index 10 (95% over 22bp)
GAATGCTACGGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGC	468	0.10845785902332307	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGC	443	0.10266416997293187	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCT	442	0.10243242241091623	Illumina PCR Primer Index 10 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7492398679965887	0.0
2	0.0	0.0	0.0	3.9691404946419966	0.0
3	0.0	0.0	0.0	4.83100967777819	0.0
4	0.0	0.0	0.0	7.032379769364827	0.0
5	0.0	0.0	0.0	12.908339204271572	0.0
6	0.0	0.0	0.0	14.009140123845897	0.0
7	0.0	0.0	0.0	15.717351403463235	0.0
8	0.0	0.0	0.0	17.734018688123403	0.0
9	0.0	0.0	0.0	18.222079053728354	0.0
10	0.0	0.0	0.0	24.5601431272943	0.0
11	0.0	0.0	0.0	26.37217731469465	0.0
12	0.0	0.0	0.0	34.24672772442434	0.0
13	0.0	0.0	0.0	35.12134302347139	0.0
14	0.0	0.0	0.0	35.577422225518184	0.0
15	0.0	0.0	0.0	37.275668359968854	0.0
16	0.0	0.0	0.0	38.304859282880344	0.0
17	0.0	0.0	0.0	39.201027105194854	0.0
18	0.0	0.0	0.0	39.946559012199195	0.0
19	0.0	0.0	0.0	42.92984537802662	0.0
20	0.0	0.0	0.0	43.76853980496125	0.0
21	0.0	0.0	0.0	44.48278579109348	0.0
22	0.0	0.0	0.0	45.37130594386147	0.0
23	0.0	0.0	0.0	46.038275427342505	0.0
24	0.0	0.0	0.0	46.581491712707184	0.0
25	0.0	0.0	0.0	47.04012013793615	0.0
26	0.0	0.0	0.0	47.49017390337054	0.0
27	0.0	0.0	0.0	47.960853201824314	0.0
28	0.0	0.0	0.0	48.40071007453002	0.0
29	0.0	0.0	0.0	48.8966498572435	0.0
30	0.0	0.0	0.0	49.39537061070118	0.0
31	0.0	0.0	0.0	49.88621194705032	0.0
32	0.0	0.0	0.0	50.30451629648856	0.0
33	0.0	0.0	0.0	50.72490637398494	0.0
34	0.0	0.0	0.0	51.138575772182875	0.0
35	0.0	0.0	0.0	51.566381771663764	0.0
36	0.0	0.0	0.0	51.99071155771441	0.0
37	0.0	0.0	0.0	52.41735881938522	0.0
38	0.0	0.0	0.0	52.830796470021134	0.0
39	0.0	0.0	0.0	53.27343431347102	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTACGG	35	1.2095734E-7	45.000004	2
ATACTCG	35	1.2095734E-7	45.000004	44
TCGCCGA	20	7.028385E-4	45.0	44
TGCCCGA	25	3.886743E-5	45.0	39
ATAGGGT	40	6.7975634E-9	45.0	24
CACGGCG	25	3.886743E-5	45.0	21
GAATGCA	20	7.028385E-4	45.0	9
ACTGACG	40	6.7975634E-9	45.0	43
TTAGCCG	20	7.028385E-4	45.0	25
TAGACCA	25	3.886743E-5	45.0	24
GCGAACC	25	3.886743E-5	45.0	33
TTTCGCG	95	0.0	45.0	1
GGACTAA	80	0.0	45.0	8
GGCCGAT	50	2.1827873E-11	45.0	8
ATGTCGT	20	7.028385E-4	45.0	38
TGTGCCG	20	7.028385E-4	45.0	1
TTGAGCG	20	7.028385E-4	45.0	15
GTGCTTA	20	7.028385E-4	45.0	21
CACTACT	25	3.886743E-5	45.0	13
ACTACGG	20	7.028385E-4	45.0	25
>>END_MODULE