SRR3549586_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549586_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	723726
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGC	38556	5.3274305469196905	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCG	29746	4.110119022945147	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTC	25466	3.5187349908667094	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGAGACTTT	11459	1.5833340242025298	No Hit
GCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC	9766	1.3494057143172968	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTA	7538	1.0415544004222594	No Hit
GAACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCT	7496	1.0357510991728915	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTC	5958	0.8232397343746114	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCT	5155	0.7122861414402689	No Hit
CTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGCT	3766	0.5203626786933176	TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC	3075	0.42488455575728934	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGAGACTTTCGT	2810	0.3882684883505636	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATG	2522	0.3484744226406126	No Hit
TCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC	2454	0.3390786015702075	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1960	0.27082072497049986	No Hit
TGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGCTT	1704	0.23544822211721012	Illumina Paired End PCR Primer 2 (95% over 22bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTAT	1701	0.2350337005993981	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTT	1560	0.2155511892622346	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCGAGACTT	1300	0.17962599105186217	No Hit
CGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTG	970	0.1340286240925433	TruSeq Adapter, Index 13 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	25	3.8893682E-5	45.0	1
TCGTTCA	25	3.8893682E-5	45.0	16
CGTATTA	20	7.0315535E-4	45.0	35
TTTACGT	25	3.8893682E-5	45.0	26
CGTGAGC	20	7.0315535E-4	45.0	16
CGATTTA	20	7.0315535E-4	45.0	12
GCCGATC	20	7.0315535E-4	45.0	9
CCCGTAT	20	7.0315535E-4	45.0	33
CGAATAC	35	1.2111013E-7	45.0	45
TCCGTTG	50	2.1827873E-11	45.0	1
ACATGCG	20	7.0315535E-4	45.0	28
TTAGTCG	20	7.0315535E-4	45.0	1
TTACGTT	20	7.0315535E-4	45.0	27
ACCCGTA	20	7.0315535E-4	45.0	45
GCAATAG	25	3.8893682E-5	45.0	9
ACGGCTG	4345	0.0	44.17146	8
TACGGCT	4360	0.0	44.019497	7
TGATACC	3430	0.0	43.55685	4
CGGCTGT	4530	0.0	43.112583	9
GATACCT	3475	0.0	42.863308	5

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content