##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549586_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	723726
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.557606055330332	30.0	16.0	31.0	16.0	34.0
2	26.573761340617857	30.0	16.0	33.0	16.0	34.0
3	27.63633474546997	31.0	25.0	33.0	16.0	34.0
4	31.615657030423115	35.0	28.0	35.0	19.0	37.0
5	33.418398675741926	35.0	33.0	37.0	28.0	37.0
6	31.991676407922334	35.0	31.0	37.0	17.0	37.0
7	33.17410732791139	35.0	32.0	35.0	28.0	37.0
8	32.44608318617819	35.0	32.0	37.0	23.0	37.0
9	34.27298452729348	35.0	32.0	39.0	28.0	39.0
10	33.3417329210226	35.0	31.0	37.0	27.0	39.0
11	33.888728607235336	35.0	32.0	38.0	27.0	39.0
12	34.20469625244913	35.0	32.0	39.0	27.0	39.0
13	34.41511842879764	35.0	33.0	38.0	27.0	39.0
14	34.40106062239024	36.0	32.0	39.0	26.0	40.0
15	34.98119177699848	37.0	33.0	39.0	27.0	40.0
16	34.70611253430165	36.0	32.0	39.0	27.0	40.0
17	34.11271807286183	36.0	32.0	38.0	27.0	40.0
18	34.38818834752379	36.0	32.0	39.0	27.0	40.0
19	34.23008293193833	36.0	32.0	39.0	27.0	40.0
20	34.28951702716221	36.0	32.0	39.0	26.0	40.0
21	33.92152140450944	36.0	32.0	39.0	25.0	40.0
22	35.090385035220514	37.0	33.0	39.0	27.0	40.0
23	35.59495444408519	37.0	34.0	39.0	29.0	40.0
24	35.83531750966526	38.0	34.0	39.0	30.0	40.0
25	33.84601769177838	36.0	32.0	39.0	24.0	40.0
26	34.67588977043798	37.0	33.0	39.0	27.0	40.0
27	34.98616879868901	37.0	33.0	39.0	27.0	40.0
28	34.98197660440553	37.0	33.0	39.0	27.0	40.0
29	35.591222368686495	38.0	34.0	40.0	29.0	40.0
30	33.305637768989925	36.0	31.0	39.0	23.0	40.0
31	34.10482420142429	36.0	31.0	39.0	25.0	40.0
32	34.575744135211394	37.0	33.0	39.0	27.0	40.0
33	35.0609277544264	37.0	34.0	39.0	27.0	40.0
34	35.53884066621898	38.0	35.0	40.0	30.0	40.0
35	35.797263052591724	38.0	35.0	40.0	30.0	41.0
36	35.5882654485261	38.0	35.0	40.0	29.0	41.0
37	35.92326515836104	38.0	35.0	40.0	30.0	41.0
38	35.14713303100897	38.0	34.0	40.0	27.0	41.0
39	35.298362916352325	38.0	34.0	40.0	29.0	41.0
40	34.59191047440606	37.0	33.0	40.0	25.0	41.0
41	34.31609752862271	37.0	32.0	39.0	25.0	40.0
42	35.340986782290535	37.0	34.0	40.0	29.0	41.0
43	35.40362789232389	38.0	34.0	40.0	29.0	40.0
44	35.97767110757386	38.0	35.0	40.0	31.0	41.0
45	36.12055805650205	38.0	35.0	40.0	31.0	41.0
46	35.85768785424318	38.0	35.0	40.0	30.0	41.0
47	35.4131356342041	38.0	34.0	40.0	28.0	41.0
48	35.60457134329843	38.0	35.0	40.0	29.0	41.0
49	35.91541135733689	38.0	35.0	40.0	31.0	41.0
50	35.65515678585542	38.0	34.0	40.0	29.0	41.0
51	35.2238747813399	37.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	11.0
11	19.0
12	23.0
13	18.0
14	30.0
15	59.0
16	133.0
17	383.0
18	1003.0
19	1945.0
20	2696.0
21	3412.0
22	3669.0
23	4167.0
24	5015.0
25	6703.0
26	9199.0
27	11969.0
28	15481.0
29	20811.0
30	28330.0
31	39110.0
32	52664.0
33	67203.0
34	80033.0
35	95801.0
36	105639.0
37	102364.0
38	59382.0
39	6445.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.24292066334497	3.4771446652462394	40.059359481350675	14.220575190058115
2	16.240953067873754	27.144941593918144	39.19743107198028	17.416674266227826
3	15.675407543738928	26.932292055280588	40.61481831521874	16.77748208576174
4	15.791888090244097	3.498699784172463	60.53437350599536	20.175038619588076
5	33.158128905138135	4.043657406255958	39.90543382440316	22.89277986420275
6	17.486452055059512	23.268612707018953	42.89482483702396	16.350110400897577
7	63.46738959219373	1.730903684543598	30.164454503499943	4.637252219762728
8	60.02478838676516	19.23808181549371	12.260164758485946	8.476965039255187
9	56.027971912021954	3.9950202148326857	13.203615733026034	26.773392140119327
10	40.890336950724446	21.404923962936248	20.761724741131314	16.94301434520799
11	33.642428211781805	21.207059025100662	26.668932717630707	18.48158004548683
12	25.88217087682355	16.276601918405582	29.49900929357243	28.342217911198436
13	24.505959437687743	19.7194794715127	38.389390459925444	17.385170630874118
14	20.34236713894485	27.829731141343544	25.91408903369507	25.91381268601653
15	14.020775818472735	23.903797846146194	41.16323028328401	20.912196052097066
16	15.93586523076413	24.56772314384173	26.02683888653993	33.46957273885421
17	16.010479103970287	27.721126503676807	34.06579285530712	22.202601537045787
18	15.613782011424213	25.006701431204625	31.46660476478667	27.912911792584488
19	16.551844206232747	28.894222399084736	27.660744535915526	26.89318885876699
20	20.620096555878884	25.828835774865073	38.62055529302526	14.930512376230784
21	22.245435427219693	33.119440230142345	26.622782655314303	18.012341687323655
22	16.500443538024058	22.23203256481044	34.19705800261425	27.070465894551255
23	19.626350303844273	31.504464396746833	28.37496511110558	20.49422018830331
24	22.513216327726237	22.976927732318583	25.30059718733333	29.209258752621846
25	15.643903908385218	35.34942782213158	22.26077272337874	26.745895546104464
26	16.777205738083197	22.87205378831215	30.164039981982132	30.18670049162252
27	23.33659423594012	28.492412874485645	23.523267092794786	24.647725796779444
28	14.62141749778231	28.41987160886855	32.47499744378398	24.483713449565165
29	24.718056280968213	20.439779695630666	24.163979185492853	30.67818483790827
30	22.329168773817713	30.569580200241525	27.28905690827744	19.812194117663314
31	23.176312582386153	26.661056808792278	21.35739216222714	28.805238446594426
32	27.364085303001414	30.71176108085104	22.737472468862524	19.18668114728502
33	19.85640974622993	25.414037909374542	23.350135272188645	31.379417072206884
34	22.671149026012607	23.1157924407856	25.205533585915113	29.00752494728668
35	23.22812777211265	21.207749894297013	28.485642356361385	27.07847997722895
36	16.98225571556086	29.330022688144407	25.516701071952646	28.171020524342083
37	27.65273045323783	22.50768937415541	29.806722433628195	20.032857738978564
38	20.714192940422205	33.100372240323	23.087881325252926	23.097553494001875
39	27.121175693563586	22.769943321091134	25.78890353531585	24.31997745002943
40	19.05859399828112	26.455454135957527	31.328292751676738	23.157659114084613
41	17.185371259288736	24.839511085687125	28.72233414303203	29.25278351199211
42	19.07379312060089	19.81551028980581	38.092454879332784	23.01824171026051
43	21.545723105153055	20.96829463084095	30.149393555019444	27.336588708986547
44	22.825212856799396	20.15099637155498	29.606508540525006	27.417282231120616
45	19.288653440666774	19.714919734816768	27.46785385629368	33.52857296822278
46	29.11004993602551	23.666553364118464	25.927768243782868	21.295628456073153
47	14.942118978729518	22.04259623117036	41.343685317371495	21.671599472728627
48	20.55667476365365	23.914437231770034	26.952189088135565	28.57669891644075
49	19.294733089594683	17.614401030224144	39.6151305880955	23.475735292085677
50	23.04062587222236	17.820832746094517	31.66142987815831	27.477111503524814
51	18.63426213788091	17.5345365511257	27.954916639722764	35.87628467127062
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	99.0
1	391.0
2	683.0
3	2331.0
4	3979.0
5	2804.0
6	1629.0
7	1695.0
8	1761.0
9	1871.0
10	1981.0
11	1964.0
12	1947.0
13	1919.0
14	1891.0
15	1703.0
16	1515.0
17	1578.0
18	1641.0
19	1647.0
20	1653.0
21	1918.5
22	2184.0
23	2424.0
24	2664.0
25	2940.0
26	4118.5
27	5021.0
28	6094.5
29	7168.0
30	8037.0
31	8906.0
32	9928.5
33	10951.0
34	12727.5
35	14504.0
36	15192.5
37	15881.0
38	17486.0
39	19091.0
40	21054.5
41	23018.0
42	25327.5
43	27637.0
44	32459.0
45	37281.0
46	106326.5
47	175372.0
48	133113.0
49	90854.0
50	83052.5
51	75251.0
52	60724.0
53	46197.0
54	39879.5
55	33562.0
56	30524.5
57	27487.0
58	25476.5
59	23466.0
60	21678.0
61	19890.0
62	17011.0
63	14132.0
64	11271.5
65	8411.0
66	6443.5
67	4476.0
68	3713.0
69	2950.0
70	2602.5
71	2255.0
72	1689.0
73	1123.0
74	990.0
75	710.5
76	564.0
77	441.5
78	319.0
79	243.5
80	168.0
81	123.5
82	79.0
83	43.5
84	8.0
85	4.5
86	1.0
87	2.0
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	723726.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.749629961630358
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.18763865580377	21.041175291198723
2	11.05697235582747	6.357677274520321
3	4.02815188771135	3.474236286028324
4	2.0210676167444857	2.324197844353525
5	1.2913472353081703	1.856287758354215
6	0.8941171631554039	1.5423322549836325
7	0.6802797782724997	1.369045432700002
8	0.5706507486786413	1.3124798289470612
9	0.48028038303413306	1.2427094961055274
>10	5.603850161170103	29.699421531364155
>50	0.10329798170710923	1.9999778873465133
>100	0.06870290292753432	3.9528875831721524
>500	0.004385291676225595	0.8549320618080533
>1k	0.0048725463069173285	3.201197358092353
>5k	0.0024362731534586643	5.030833219025498
>10k+	0.0019490185227669312	14.740608892000001
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGC	38556	5.3274305469196905	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCG	29746	4.110119022945147	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTC	25466	3.5187349908667094	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGAGACTTT	11459	1.5833340242025298	No Hit
GCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC	9766	1.3494057143172968	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTA	7538	1.0415544004222594	No Hit
GAACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCT	7496	1.0357510991728915	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTC	5958	0.8232397343746114	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCT	5155	0.7122861414402689	No Hit
CTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGCT	3766	0.5203626786933176	TruSeq Adapter, Index 13 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC	3075	0.42488455575728934	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGAGACTTTCGT	2810	0.3882684883505636	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATG	2522	0.3484744226406126	No Hit
TCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGC	2454	0.3390786015702075	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1960	0.27082072497049986	No Hit
TGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTGCTT	1704	0.23544822211721012	Illumina Paired End PCR Primer 2 (95% over 22bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTAT	1701	0.2350337005993981	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTT	1560	0.2155511892622346	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCGAGACTT	1300	0.17962599105186217	No Hit
CGCTGTCTCTTATACACATCTGACGCCGAGACTTTCGTATGCCGTCTTCTG	970	0.1340286240925433	TruSeq Adapter, Index 13 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.381738392706632E-4	0.0	0.0	0.5767376051157482	0.0
2	1.381738392706632E-4	0.0	0.0	3.0591688014524836	0.0
3	1.381738392706632E-4	0.0	0.0	3.696564722008053	0.0
4	1.381738392706632E-4	0.0	0.0	5.816013242580755	0.0
5	1.381738392706632E-4	0.0	0.0	10.872761238369216	0.0
6	1.381738392706632E-4	0.0	0.0	11.738282167560651	0.0
7	1.381738392706632E-4	0.0	0.0	13.13604872562268	0.0
8	1.381738392706632E-4	0.0	0.0	14.97541887399375	0.0
9	1.381738392706632E-4	0.0	0.0	15.393809259305318	0.0
10	1.381738392706632E-4	0.0	0.0	20.705487988548153	0.0
11	1.381738392706632E-4	0.0	0.0	22.480192780140552	0.0
12	1.381738392706632E-4	0.0	0.0	29.842094936481487	0.0
13	1.381738392706632E-4	0.0	0.0	30.697391001566892	0.0
14	1.381738392706632E-4	0.0	0.0	31.12061747125293	0.0
15	1.381738392706632E-4	0.0	0.0	32.575311651094474	0.0
16	1.381738392706632E-4	0.0	0.0	33.51572280117061	0.0
17	1.381738392706632E-4	0.0	0.0	34.347943835097816	0.0
18	1.381738392706632E-4	0.0	0.0	35.07432370814369	0.0
19	1.381738392706632E-4	0.0	0.0	37.512124754396	0.0
20	1.381738392706632E-4	0.0	0.0	38.373776816087855	0.0
21	1.381738392706632E-4	0.0	0.0	39.246344611082094	0.0
22	1.381738392706632E-4	0.0	0.0	40.27601606132708	0.0
23	1.381738392706632E-4	0.0	0.0	41.14540585801809	0.0
24	1.381738392706632E-4	0.0	0.0	41.82079958437309	0.0
25	1.381738392706632E-4	0.0	0.0	42.427935434128386	0.0
26	1.381738392706632E-4	0.0	0.0	42.979525400496875	0.0
27	1.381738392706632E-4	0.0	0.0	43.52406850106256	0.0
28	1.381738392706632E-4	0.0	0.0	44.05064900252305	0.0
29	1.381738392706632E-4	0.0	0.0	44.60403522880206	0.0
30	1.381738392706632E-4	0.0	0.0	45.20882212328976	0.0
31	1.381738392706632E-4	0.0	0.0	45.81070736715276	0.0
32	1.381738392706632E-4	0.0	0.0	46.38067445414425	0.0
33	1.381738392706632E-4	0.0	0.0	46.90739312944402	0.0
34	1.381738392706632E-4	0.0	0.0	47.4462711025996	0.0
35	1.381738392706632E-4	0.0	0.0	47.964146652186045	0.0
36	1.381738392706632E-4	0.0	0.0	48.49749767177081	0.0
37	1.381738392706632E-4	0.0	0.0	49.03485573269442	0.0
38	1.381738392706632E-4	0.0	0.0	49.60896803486402	0.0
39	1.381738392706632E-4	0.0	0.0	50.22950674702857	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	25	3.8893682E-5	45.0	1
TCGTTCA	25	3.8893682E-5	45.0	16
CGTATTA	20	7.0315535E-4	45.0	35
TTTACGT	25	3.8893682E-5	45.0	26
CGTGAGC	20	7.0315535E-4	45.0	16
CGATTTA	20	7.0315535E-4	45.0	12
GCCGATC	20	7.0315535E-4	45.0	9
CCCGTAT	20	7.0315535E-4	45.0	33
CGAATAC	35	1.2111013E-7	45.0	45
TCCGTTG	50	2.1827873E-11	45.0	1
ACATGCG	20	7.0315535E-4	45.0	28
TTAGTCG	20	7.0315535E-4	45.0	1
TTACGTT	20	7.0315535E-4	45.0	27
ACCCGTA	20	7.0315535E-4	45.0	45
GCAATAG	25	3.8893682E-5	45.0	9
ACGGCTG	4345	0.0	44.17146	8
TACGGCT	4360	0.0	44.019497	7
TGATACC	3430	0.0	43.55685	4
CGGCTGT	4530	0.0	43.112583	9
GATACCT	3475	0.0	42.863308	5
>>END_MODULE