FastQCFastQC Report
Sat 18 Jun 2016
SRR3549585_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549585_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences612983
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGC391926.393652026238901No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCG323875.283507046688081No Hit
GAATCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTC288724.710081682526269No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTATAGCGT121661.9847206203108405No Hit
GCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC118371.93104865877194No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTATAGCGTCGTA84851.3842145703877595No Hit
GAACTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCT83061.3550131080307284No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTC68271.1137339860974937No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCT62171.0142206227578905No Hit
CTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGCT47460.7742465941143555No Hit
CCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC42120.6871316170268995No Hit
TCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC35520.5794614206266732No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTATAGCGTCGT28920.47179122422644676No Hit
TGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGCTT27080.44177407856335327Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATG26450.4314964689069681No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTAT18550.30261850654912126No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTT15850.25857160802175594No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15030.24519440180233382No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTATAGCG14150.230838375615637No Hit
CGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTG12750.20799924304589198No Hit
GCTGTCTCTTATACACATCTGACGCCTATCGCGTCGTATGCCGTCTTCTGC9510.15514296481305354No Hit
TCCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTG9010.14698613175243033No Hit
TTCCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCT8590.14013439198150682No Hit
TGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTG8390.13687165875725754No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGC7940.12953050900269664No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCTATAGCGTCG7330.11957917266873634No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGC6680.10897528968992615No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGA253.888668E-545.030
TCTTGCG850.045.01
ACCGGTG1700.045.017
GTCGATA406.8066583E-945.015
GAGTCTA253.888668E-545.09
CCGATCA207.030708E-445.040
TCTTACG207.030708E-445.01
CACGCCA207.030708E-445.026
ACCGAAC207.030708E-445.010
TCGATAT406.8066583E-945.016
ACGGGGT253.888668E-545.05
GCCGCGT207.030708E-445.010
CAGGCGT253.888668E-545.033
CCGACCC253.888668E-545.036
CCGCGTC207.030708E-445.011
CGCTTGG1000.045.02
CTTACGG207.030708E-445.02
GTCGAAT302.1638389E-644.99999643
TCTCTCG302.1638389E-644.9999961
ACGGGAC302.1638389E-644.9999965
Produced by FastQC (version 0.11.5)