##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549585_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	612983
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.09944810867512	28.0	16.0	31.0	16.0	34.0
2	25.922630807053377	30.0	16.0	33.0	16.0	34.0
3	26.931689785850505	31.0	16.0	33.0	16.0	34.0
4	31.222296540034552	33.0	28.0	35.0	19.0	37.0
5	33.319181771762025	35.0	32.0	35.0	28.0	37.0
6	31.762720988999696	35.0	30.0	37.0	17.0	37.0
7	33.25092539271073	35.0	32.0	35.0	28.0	37.0
8	32.30199043040345	35.0	32.0	37.0	19.0	37.0
9	34.30229386459331	35.0	32.0	39.0	28.0	39.0
10	33.68461278697778	35.0	32.0	37.0	27.0	39.0
11	34.40699007965963	35.0	32.0	39.0	27.0	39.0
12	34.5027725075573	37.0	34.0	39.0	27.0	39.0
13	34.78031527791146	37.0	34.0	39.0	30.0	39.0
14	34.599065553204575	37.0	33.0	39.0	27.0	40.0
15	35.2030757786105	37.0	33.0	39.0	27.0	40.0
16	34.70883531843461	36.0	32.0	39.0	27.0	40.0
17	34.22896719811153	36.0	32.0	38.0	27.0	40.0
18	34.481187895912285	36.0	32.0	39.0	27.0	40.0
19	34.24028725103306	36.0	32.0	39.0	27.0	40.0
20	34.30582740467517	36.0	32.0	39.0	27.0	40.0
21	34.10703070068827	36.0	32.0	39.0	25.0	40.0
22	35.31844276268673	37.0	34.0	39.0	29.0	40.0
23	35.685586712845215	37.0	34.0	39.0	30.0	40.0
24	35.989655504312516	38.0	34.0	39.0	30.0	40.0
25	33.993954155335466	37.0	32.0	39.0	24.0	40.0
26	34.99311889563006	37.0	33.0	39.0	27.0	40.0
27	35.36035746505205	37.0	34.0	39.0	29.0	40.0
28	35.20824068530449	37.0	34.0	39.0	27.0	40.0
29	35.81670780429474	38.0	34.0	40.0	30.0	40.0
30	33.550530765127256	36.0	31.0	39.0	23.0	40.0
31	34.66963357874525	37.0	33.0	39.0	25.0	40.0
32	35.14253086953472	37.0	34.0	39.0	29.0	40.0
33	35.47173902049486	38.0	34.0	39.0	29.0	40.0
34	35.80193741098856	38.0	35.0	40.0	30.0	40.0
35	35.97628156082632	38.0	35.0	40.0	30.0	41.0
36	35.89088278141482	38.0	35.0	40.0	30.0	41.0
37	36.208421766998434	38.0	35.0	40.0	30.0	41.0
38	35.67852942088117	38.0	35.0	40.0	30.0	41.0
39	35.71069997047226	38.0	34.0	40.0	30.0	41.0
40	35.264524464789396	37.0	34.0	40.0	28.0	41.0
41	34.7802532859802	37.0	33.0	39.0	26.0	40.0
42	35.70172419137236	38.0	35.0	40.0	30.0	40.0
43	35.82950098126702	38.0	35.0	40.0	30.0	40.0
44	36.36383227593588	38.0	35.0	40.0	31.0	41.0
45	36.61650975638803	39.0	35.0	40.0	32.0	41.0
46	36.33029137839059	39.0	35.0	40.0	31.0	41.0
47	35.81148743113594	38.0	35.0	40.0	28.0	41.0
48	36.08176898869952	38.0	35.0	40.0	31.0	41.0
49	36.38034170605057	39.0	35.0	40.0	31.0	41.0
50	36.06505890049153	38.0	35.0	40.0	29.0	41.0
51	35.59850110035678	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	8.0
11	9.0
12	9.0
13	7.0
14	22.0
15	37.0
16	95.0
17	285.0
18	579.0
19	1141.0
20	1637.0
21	2221.0
22	2435.0
23	2725.0
24	3371.0
25	4723.0
26	6715.0
27	9002.0
28	12038.0
29	16614.0
30	22469.0
31	30956.0
32	42334.0
33	56415.0
34	71364.0
35	88485.0
36	94888.0
37	87354.0
38	49904.0
39	5138.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.65558914358147	3.2493560180298635	38.28148578345566	12.813569054933009
2	14.329924320902865	32.13661716556577	36.3815962269753	17.15186228655607
3	13.60151912858921	32.19632518356953	38.44070716479903	15.761448523042237
4	15.102050138421458	2.9707186006789748	63.25477215518212	18.67245910571745
5	35.79577247656134	3.603362572860911	38.303835506041764	22.297029444535983
6	16.073039545958046	25.952758885646094	41.1143539054101	16.85984766298576
7	58.46067509213143	1.1223802291417542	34.9614915911208	5.455453087606019
8	53.428072230388125	21.566340338965354	14.318178481295565	10.687408949350962
9	50.069577786007116	4.042037054861228	14.709217058221844	31.179168100909816
10	38.247553357923465	23.197054404445147	21.96651456891953	16.588877668711856
11	29.932640872585374	19.417177964152348	30.91227652316622	19.737904640096055
12	20.86289505581721	15.494230671976222	33.857382668034845	29.78549160417173
13	21.08932874158011	21.041529699844858	42.65860554044728	15.210536018127746
14	18.786981041888602	29.496902850486883	26.2643499085619	25.451766199062615
15	10.554778843785227	23.95857633898493	45.03876942753714	20.447875389692697
16	11.996580655580987	25.783259894646342	25.664822678606097	36.55533677116657
17	13.050117213691081	28.6595223684833	35.00765926624393	23.28270115158169
18	12.183208996008046	24.91635167696331	34.05330979815101	28.847129528877634
19	13.57182825624854	27.809743500227572	29.484178190912306	29.13425005261157
20	15.638117207165614	26.69992479399918	42.97313302326492	14.688824975570286
21	19.594833788212725	34.23912245527201	27.985115411030975	18.180928345484297
22	14.28930329226096	22.1048544576277	35.00896435953362	28.596877890577716
23	16.26864040275179	32.547721551821176	29.450898312024965	21.732739733402067
24	21.776297221945796	22.29474553127901	25.131039523118915	30.797917723656283
25	12.64619084052902	37.623229355463366	22.50894396745097	27.22163583655664
26	14.68654106231331	22.23454810329161	30.390239207286335	32.68867162710875
27	19.63105012700189	28.450870578792557	26.607915717075354	25.310163577130197
28	10.64695105737027	28.65397572200208	35.375369300616825	25.323703920010832
29	18.567725369219048	18.75843212617642	30.17995605098347	32.493886453621066
30	14.300722858545834	32.81379744625871	34.22182996918349	18.663649726011975
31	23.157085922448097	26.764363775178108	23.184982291515425	26.893568010858377
32	20.633524910152484	31.140178438880035	26.63564894948147	21.590647701486013
33	19.969069289034117	26.224218289903632	22.307633327514793	31.499079093547454
34	28.4042134936858	21.744322436348153	23.50701406074883	26.344450009217223
35	16.829014834016604	19.717349420783282	29.502449496968104	33.951186248232005
36	22.981061464999843	27.46307809515109	21.860965149115067	27.694895290734
37	16.213826484584402	19.98913509836325	44.776119402985074	19.020919014067275
38	18.329872117171277	35.237845095214716	21.702559451077764	24.72972333653625
39	27.64448606241935	22.056729142570024	32.79732064347625	17.501464151534382
40	17.790542315202867	26.984761404476142	28.157713998593763	27.066982281727224
41	30.68486401743605	24.26984108857831	21.32652944698303	23.71876544700261
42	18.999711248109655	17.22119536757137	33.32049991598462	30.45859346833436
43	29.96592075147272	19.28014316873388	22.453477502638737	28.300458577154668
44	21.13990110655597	17.966729909312328	34.20127474987071	26.69209423426098
45	18.139328496875116	17.82822688394295	28.274193574699463	35.75825104448248
46	30.40312700352212	22.158852692489024	28.059995138527494	19.37802516546136
47	13.097753118765121	19.782767221929483	45.24742121722788	21.872058442077513
48	22.460655515732082	23.190855211319075	26.35489075553482	27.993598517414025
49	18.336397583619775	15.293572578684891	41.05219883748815	25.317831000207185
50	26.506934123784838	15.090956845459008	30.616999166371663	27.785109864384495
51	19.343929603267952	15.475306819275575	27.546930339014295	37.63383323844218
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	110.0
1	268.5
2	427.0
3	1484.0
4	2541.0
5	1771.0
6	1001.0
7	1175.0
8	1349.0
9	1530.5
10	1712.0
11	1798.5
12	1885.0
13	1767.0
14	1649.0
15	1540.0
16	1431.0
17	1485.0
18	1539.0
19	1539.5
20	1540.0
21	1802.0
22	2064.0
23	2289.0
24	2514.0
25	3061.0
26	4327.0
27	5046.0
28	5419.0
29	5792.0
30	6731.5
31	7671.0
32	8638.5
33	9606.0
34	10645.0
35	11684.0
36	12367.5
37	13051.0
38	14548.0
39	16045.0
40	18494.5
41	20944.0
42	22977.0
43	25010.0
44	29330.0
45	33650.0
46	108158.5
47	182667.0
48	136072.0
49	89477.0
50	78736.5
51	67996.0
52	50652.5
53	33309.0
54	26372.0
55	19435.0
56	16863.0
57	14291.0
58	12578.0
59	10865.0
60	9773.5
61	8682.0
62	7296.5
63	5911.0
64	4672.0
65	3433.0
66	2731.0
67	2029.0
68	1609.5
69	1190.0
70	1007.5
71	825.0
72	686.0
73	547.0
74	393.5
75	176.5
76	113.0
77	97.5
78	82.0
79	50.0
80	18.0
81	10.5
82	3.0
83	1.5
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	612983.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.719051866078495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.79951740106199	18.466155119973187
2	11.784879725460213	6.061918657892163
3	4.594729533905628	3.5451626157936458
4	2.307897299653857	2.374277214055202
5	1.3513401252543114	1.7377593385064325
6	0.9364086349512931	1.4450125350093643
7	0.7088167983060544	1.2761067199426779
8	0.5471291144087151	1.125731365673547
9	0.5138104383801277	1.1893245582627932
>10	5.236648609546471	24.687553919138598
>50	0.11679079786213553	2.1156622607520466
>100	0.08085516775070921	4.338436215647792
>500	0.008342199847295395	1.4876807565659524
>1k	0.0070587844861730266	4.685187632282479
>5k	0.002566830722244737	4.924002149117506
>10k+	0.0032085384028059214	20.54002894138663
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGC	39192	6.393652026238901	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCG	32387	5.283507046688081	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTC	28872	4.710081682526269	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTATAGCGT	12166	1.9847206203108405	No Hit
GCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	11837	1.93104865877194	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTATAGCGTCGTA	8485	1.3842145703877595	No Hit
GAACTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCT	8306	1.3550131080307284	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTC	6827	1.1137339860974937	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCT	6217	1.0142206227578905	No Hit
CTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGCT	4746	0.7742465941143555	No Hit
CCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	4212	0.6871316170268995	No Hit
TCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGC	3552	0.5794614206266732	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTATAGCGTCGT	2892	0.47179122422644676	No Hit
TGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTGCTT	2708	0.44177407856335327	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATG	2645	0.4314964689069681	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTAT	1855	0.30261850654912126	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTT	1585	0.25857160802175594	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1503	0.24519440180233382	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTATAGCG	1415	0.230838375615637	No Hit
CGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTG	1275	0.20799924304589198	No Hit
GCTGTCTCTTATACACATCTGACGCCTATCGCGTCGTATGCCGTCTTCTGC	951	0.15514296481305354	No Hit
TCCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTG	901	0.14698613175243033	No Hit
TTCCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCT	859	0.14013439198150682	No Hit
TGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGCCGTCTTCTG	839	0.13687165875725754	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGC	794	0.12953050900269664	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCTATAGCGTCG	733	0.11957917266873634	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCTATAGCGTCGTATGC	668	0.10897528968992615	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8708234975521344	0.0
2	0.0	0.0	0.0	4.594580926387844	0.0
3	0.0	0.0	0.0	5.580905180078403	0.0
4	0.0	0.0	0.0	8.453904920691112	0.0
5	0.0	0.0	0.0	15.335335563955281	0.0
6	0.0	0.0	0.0	16.478271012409806	0.0
7	0.0	0.0	0.0	18.431016847123004	0.0
8	0.0	0.0	0.0	20.864689559090547	0.0
9	0.0	0.0	0.0	21.388358241582555	0.0
10	1.631366612124643E-4	0.0	0.0	28.08022408451784	0.0
11	1.631366612124643E-4	0.0	0.0	30.11111237995181	0.0
12	1.631366612124643E-4	0.0	0.0	38.894390219630885	0.0
13	1.631366612124643E-4	0.0	0.0	39.894744226185715	0.0
14	1.631366612124643E-4	0.0	0.0	40.390516539610395	0.0
15	1.631366612124643E-4	0.0	0.0	42.23363453798882	0.0
16	1.631366612124643E-4	0.0	0.0	43.29206519593529	0.0
17	1.631366612124643E-4	0.0	0.0	44.3261884913611	0.0
18	1.631366612124643E-4	0.0	0.0	45.206637051924766	0.0
19	1.631366612124643E-4	0.0	0.0	48.21324571807048	0.0
20	1.631366612124643E-4	0.0	0.0	49.19418646194103	0.0
21	1.631366612124643E-4	0.0	0.0	50.06664132610529	0.0
22	1.631366612124643E-4	0.0	0.0	51.08738741531168	0.0
23	1.631366612124643E-4	0.0	0.0	51.98610728193115	0.0
24	1.631366612124643E-4	0.0	0.0	52.64159038668283	0.0
25	1.631366612124643E-4	0.0	0.0	53.19707071811127	0.0
26	1.631366612124643E-4	0.0	0.0	53.74439421647909	0.0
27	1.631366612124643E-4	0.0	0.0	54.27801423530506	0.0
28	1.631366612124643E-4	0.0	0.0	54.785042978353395	0.0
29	1.631366612124643E-4	0.0	0.0	55.2941924979975	0.0
30	1.631366612124643E-4	0.0	0.0	55.89975578441816	0.0
31	1.631366612124643E-4	0.0	0.0	56.45083142599387	0.0
32	1.631366612124643E-4	0.0	0.0	56.938773179680354	0.0
33	1.631366612124643E-4	0.0	0.0	57.43829763631292	0.0
34	1.631366612124643E-4	0.0	0.0	57.91906137690605	0.0
35	1.631366612124643E-4	0.0	0.0	58.4210328834568	0.0
36	1.631366612124643E-4	0.0	0.0	58.94796429917306	0.0
37	1.631366612124643E-4	0.0	0.0	59.43965819606743	0.0
38	1.631366612124643E-4	0.0	0.0	59.931352092961795	0.0
39	1.631366612124643E-4	0.0	0.0	60.428592636337385	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTGCGA	25	3.888668E-5	45.0	30
TCTTGCG	85	0.0	45.0	1
ACCGGTG	170	0.0	45.0	17
GTCGATA	40	6.8066583E-9	45.0	15
GAGTCTA	25	3.888668E-5	45.0	9
CCGATCA	20	7.030708E-4	45.0	40
TCTTACG	20	7.030708E-4	45.0	1
CACGCCA	20	7.030708E-4	45.0	26
ACCGAAC	20	7.030708E-4	45.0	10
TCGATAT	40	6.8066583E-9	45.0	16
ACGGGGT	25	3.888668E-5	45.0	5
GCCGCGT	20	7.030708E-4	45.0	10
CAGGCGT	25	3.888668E-5	45.0	33
CCGACCC	25	3.888668E-5	45.0	36
CCGCGTC	20	7.030708E-4	45.0	11
CGCTTGG	100	0.0	45.0	2
CTTACGG	20	7.030708E-4	45.0	2
GTCGAAT	30	2.1638389E-6	44.999996	43
TCTCTCG	30	2.1638389E-6	44.999996	1
ACGGGAC	30	2.1638389E-6	44.999996	5
>>END_MODULE