SRR3549584_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549584_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	480013
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGC	26170	5.451935676742088	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCG	22418	4.670290179640968	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTC	15383	3.204704872576368	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCATTCGT	8183	1.704745496476137	No Hit
GCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC	6222	1.2962148941799492	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTA	4633	0.9651821929822734	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCT	3905	0.8135196338432502	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTC	3502	0.7295635743198622	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCT	3380	0.7041475960026082	No Hit
CTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGCT	1758	0.3662400809978063	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGCATTCGTCGT	1671	0.34811557186992853	No Hit
CCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC	1516	0.31582477974554857	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATG	1429	0.2977002706176708	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1386	0.2887421798992944	No Hit
TCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC	1309	0.2727009476826669	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTAT	1022	0.21291090032978274	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGCATTCG	989	0.20603608652265668	No Hit
TGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGCTT	909	0.1893698712326541	Illumina Single End Adapter 1 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTT	902	0.1879115773947789	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTG	540	0.11249695320751729	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATGAATGAT	505	0.10520548401814118	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGGC	40	6.8012014E-9	45.000004	28
ATTCGAT	20	7.029176E-4	45.000004	24
TCTAGCG	20	7.029176E-4	45.000004	1
TCGTGCG	35	1.2099554E-7	45.000004	1
TGATTCG	35	1.2099554E-7	45.000004	26
GTGTATC	20	7.029176E-4	45.000004	23
GATTATC	20	7.029176E-4	45.000004	9
TATGTCG	20	7.029176E-4	45.000004	1
TCGCGCG	20	7.029176E-4	45.000004	1
ATTGGGT	25	3.8873994E-5	45.0	4
CCTTGCG	25	3.8873994E-5	45.0	1
CGGGTAT	60	0.0	44.999996	6
TGGTTCG	30	2.1628512E-6	44.999996	1
CAATCAT	30	2.1628512E-6	44.999996	45
TACGGCT	2920	0.0	43.998287	7
TGATACC	2430	0.0	43.51852	4
GATACCT	2425	0.0	43.515465	5
ACGGCTG	2980	0.0	43.11242	8
GACGAAT	85	0.0	42.35294	20
ATACCTG	2500	0.0	42.3	6

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content