##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549584_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	480013
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.58525081612373	30.0	16.0	33.0	16.0	34.0
2	26.54052911066992	30.0	16.0	33.0	16.0	34.0
3	27.59426932187253	31.0	25.0	33.0	16.0	34.0
4	31.571611602185776	35.0	28.0	35.0	19.0	37.0
5	33.35497371946177	35.0	33.0	37.0	28.0	37.0
6	31.847516629757944	35.0	30.0	37.0	17.0	37.0
7	33.09975563161831	35.0	32.0	35.0	28.0	37.0
8	32.302387643667984	35.0	32.0	37.0	17.0	37.0
9	34.27160722730426	35.0	32.0	38.0	28.0	39.0
10	33.16256226393868	35.0	31.0	37.0	27.0	39.0
11	33.539189563615984	35.0	32.0	37.0	27.0	39.0
12	33.968863343284454	35.0	32.0	39.0	26.0	39.0
13	34.10414926262414	35.0	32.0	37.0	27.0	39.0
14	34.174182782549636	36.0	32.0	39.0	25.0	40.0
15	34.74928804011558	36.0	32.0	39.0	27.0	40.0
16	34.72668031907469	36.0	32.0	39.0	27.0	40.0
17	34.054977677687894	36.0	32.0	38.0	26.0	40.0
18	34.32013716295184	36.0	32.0	39.0	27.0	40.0
19	34.2371164947616	36.0	32.0	39.0	27.0	40.0
20	34.041048888259276	36.0	32.0	39.0	25.0	40.0
21	33.69256457637606	36.0	32.0	39.0	25.0	40.0
22	34.81335297169035	37.0	33.0	39.0	27.0	40.0
23	35.321541291590016	37.0	33.0	39.0	29.0	40.0
24	35.667950659669636	37.0	34.0	39.0	30.0	40.0
25	33.86255580578026	36.0	32.0	39.0	24.0	40.0
26	34.52794820140288	36.0	33.0	39.0	27.0	40.0
27	34.69663946601446	37.0	33.0	39.0	27.0	40.0
28	34.8835802363686	37.0	33.0	39.0	27.0	40.0
29	35.46326037003165	38.0	34.0	40.0	28.0	40.0
30	33.63078708284984	36.0	32.0	39.0	23.0	40.0
31	34.29742111151156	36.0	32.0	39.0	25.0	40.0
32	34.45067529421078	37.0	33.0	39.0	26.0	40.0
33	35.05488393022689	37.0	34.0	39.0	27.0	40.0
34	35.29821692329166	38.0	34.0	40.0	27.0	40.0
35	35.50183224204344	38.0	35.0	40.0	27.0	41.0
36	35.281159051942346	38.0	34.0	40.0	26.0	41.0
37	35.475143381533414	38.0	35.0	40.0	29.0	41.0
38	35.28543601944114	38.0	34.0	40.0	29.0	41.0
39	35.12527577378113	37.0	34.0	40.0	28.0	41.0
40	34.8187694916596	37.0	33.0	40.0	26.0	41.0
41	34.43721315881028	37.0	33.0	39.0	25.0	40.0
42	35.123571653267724	38.0	34.0	40.0	28.0	41.0
43	35.104457587607	38.0	34.0	40.0	28.0	41.0
44	35.67681708620392	38.0	35.0	40.0	30.0	41.0
45	35.861153760418986	38.0	35.0	40.0	30.0	41.0
46	35.667898577746854	38.0	35.0	40.0	30.0	41.0
47	35.12307999991667	38.0	34.0	40.0	26.0	41.0
48	35.50044061306673	38.0	35.0	40.0	29.0	41.0
49	35.65591973550716	38.0	35.0	40.0	29.0	41.0
50	35.34815307085433	38.0	35.0	40.0	28.0	41.0
51	34.9281331963926	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	11.0
11	11.0
12	15.0
13	18.0
14	21.0
15	50.0
16	112.0
17	388.0
18	987.0
19	2111.0
20	3002.0
21	3528.0
22	3771.0
23	3453.0
24	3668.0
25	4399.0
26	5784.0
27	7689.0
28	10227.0
29	13504.0
30	18623.0
31	25437.0
32	34217.0
33	44803.0
34	51385.0
35	60916.0
36	67375.0
37	67706.0
38	42109.0
39	4687.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.61155427040518	3.4894888263442865	39.49726361577707	14.401693287473464
2	15.738115426040544	28.186528281525707	38.86686402243272	17.20849227000102
3	15.349167626710111	28.419855295585744	39.60434404901534	16.626633028688804
4	15.103965934255948	3.6638591038159385	62.27248012033007	18.95969484159804
5	34.755933693462474	4.2765508434146575	39.21893782043403	21.748577642688844
6	17.745977713103603	25.060362948503478	41.661579998875034	15.532079339517887
7	63.08454146033545	1.5664159095691157	30.775833154518732	4.573209475576704
8	60.99918127217387	20.967348800969972	11.008035199046692	7.025434727809456
9	57.2201169551658	4.040932224752247	11.114490649211584	27.624460170870375
10	43.299035651117784	22.2766883396908	18.351794638895196	16.07248137029622
11	37.51398399626677	20.074247989116962	25.358271546812272	17.053496467803996
12	29.216083731065616	15.647701208092279	26.57782185065821	28.55839321018389
13	27.66612570909538	18.18888238443542	36.80650315720616	17.33848874926304
14	20.926933228891716	28.830260847102057	24.532668906883774	25.710137017122452
15	14.274196740504944	21.99461264590751	40.94764100138954	22.783549612198005
16	17.163493488718014	22.86042253022314	25.84992489786735	34.126159083191496
17	17.545358146550196	27.680500319783008	33.48346815607077	21.290673377596022
18	16.38205631930802	24.23871853470635	30.390635253628545	28.98858989235708
19	18.008887259303393	27.904035932360166	26.91510438258964	27.171972425746805
20	24.152054215198337	25.372854485191027	36.12339665800718	14.351694641603457
21	23.32957649063671	34.35365292190003	25.180151370900372	17.136619216562885
22	18.0480528652349	21.124427880078247	34.38344378173091	26.44407547295594
23	22.019403641151385	31.207071475147547	27.45592306875022	19.317601814950844
24	23.251036951082575	22.671052658990487	25.39660384197928	28.68130654794766
25	18.596788003658236	35.22154608312692	21.833991996050106	24.347673917164744
26	18.425750969244582	21.649830317095578	32.89473410095143	27.029684612708405
27	24.281842366769233	27.321135052592325	24.140387864495334	24.256634716143104
28	14.405859841295964	27.767997950055516	34.07428548810137	23.751856720547153
29	22.816673715086882	21.624830994160575	31.25144527335718	24.307050017395362
30	21.840033499092733	28.136529635655698	30.2554305820884	19.768006283163164
31	23.03708441229717	21.329630655836404	24.15455414749184	31.47873078437459
32	26.996977165201773	31.72143254453525	22.917712645282524	18.363877644980448
33	23.776856043482155	20.119455098091095	26.204498628162153	29.8991902302646
34	24.03414074202157	21.575457331363943	33.13035271961384	21.260049207000645
35	24.52850235306127	21.494209531825177	25.3876457512609	28.589642363852647
36	20.86651819846546	24.660373781543417	29.418786574530277	25.054321445460854
37	24.223093957871974	26.8432313291515	28.96317391403983	19.970500798936698
38	21.18671785972463	22.55980567192972	24.95578244755871	31.29769402078694
39	21.34442192190628	29.346913521092137	30.078768700014376	19.229895856987206
40	19.30385218733659	19.58655286419326	33.48680139912877	27.622793549341374
41	23.06875022134817	22.36814419609469	29.661904990073186	24.901200592483956
42	19.507388341565747	20.190286513073605	29.73565299273145	30.5666721526292
43	27.539254145200232	20.517777643522155	25.82805049029922	26.114917720978394
44	24.949324289133838	18.824906825440145	31.750598421292757	24.475170464133264
45	19.596969248749513	17.2399497513609	28.06153166685069	35.101549333038896
46	28.181945072320957	26.733234308237485	24.25600973306973	20.828810886371826
47	15.819988208652683	20.89839233520759	42.4019766131334	20.879642843006334
48	22.04981948405564	22.66667777747686	28.547976825627643	26.735525912839858
49	19.741965321772536	17.02870547256012	37.03545528975257	26.19387391591478
50	25.152443787980744	18.276588342399062	29.39399557928639	27.1769722903338
51	19.373641963863477	17.973679879503262	28.649432411205527	34.003245745427726
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	56.0
1	213.0
2	370.0
3	1695.5
4	3021.0
5	2093.0
6	1165.0
7	1180.5
8	1196.0
9	1235.5
10	1275.0
11	1272.5
12	1270.0
13	1236.5
14	1203.0
15	1092.0
16	981.0
17	1040.0
18	1099.0
19	1119.0
20	1139.0
21	1299.5
22	1460.0
23	1582.5
24	1705.0
25	1945.0
26	2725.5
27	3266.0
28	3699.0
29	4132.0
30	4951.5
31	5771.0
32	6332.5
33	6894.0
34	7717.0
35	8540.0
36	9335.0
37	10130.0
38	11606.0
39	13082.0
40	13865.5
41	14649.0
42	16607.0
43	18565.0
44	20872.5
45	23180.0
46	68400.0
47	113620.0
48	82938.5
49	52257.0
50	48403.0
51	44549.0
52	36726.5
53	28904.0
54	26425.5
55	23947.0
56	22758.0
57	21569.0
58	20462.0
59	19355.0
60	17867.0
61	16379.0
62	14734.0
63	13089.0
64	10686.5
65	8284.0
66	6513.0
67	4742.0
68	3652.0
69	2562.0
70	2236.5
71	1911.0
72	1507.0
73	1103.0
74	833.5
75	464.5
76	365.0
77	272.0
78	179.0
79	172.5
80	166.0
81	143.5
82	121.0
83	65.5
84	10.0
85	6.5
86	3.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	480013.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.03753403054533
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.82348110371126	20.978658977291698
2	9.736829057620259	5.459933521052612
3	3.341691405975716	2.81078359543875
4	1.737983955867569	1.94915137228715
5	1.0291509042756484	1.4427426750597492
6	0.7999798816915642	1.3457677894007314
7	0.6258894046633273	1.228387683782406
8	0.6010660411081644	1.3481927665740256
9	0.545701143197688	1.3770102935621404
>10	6.588542810854473	34.88004779064003
>50	0.0994185493725508	1.8946283225680534
>100	0.05456807597140006	2.705923260251785
>500	0.0037375394500958944	0.8058457854285581
>1k	0.008222586790210968	5.346666276220532
>5k	0.0014950157800383578	3.019039932145222
>10k+	0.002242523670057537	13.407219958296565
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGC	26170	5.451935676742088	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCG	22418	4.670290179640968	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTC	15383	3.204704872576368	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCATTCGT	8183	1.704745496476137	No Hit
GCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC	6222	1.2962148941799492	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTA	4633	0.9651821929822734	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCT	3905	0.8135196338432502	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTC	3502	0.7295635743198622	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCT	3380	0.7041475960026082	No Hit
CTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGCT	1758	0.3662400809978063	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGCATTCGTCGT	1671	0.34811557186992853	No Hit
CCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC	1516	0.31582477974554857	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATG	1429	0.2977002706176708	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1386	0.2887421798992944	No Hit
TCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC	1309	0.2727009476826669	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTAT	1022	0.21291090032978274	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGCATTCG	989	0.20603608652265668	No Hit
TGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGCTT	909	0.1893698712326541	Illumina Single End Adapter 1 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTT	902	0.1879115773947789	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTG	540	0.11249695320751729	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATGAATGAT	505	0.10520548401814118	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4108222068985632	0.0
2	0.0	0.0	0.0	2.438683952309625	0.0
3	0.0	0.0	0.0	2.9582532139754547	0.0
4	0.0	0.0	0.0	4.424671831804555	0.0
5	0.0	0.0	0.0	8.797261740827853	0.0
6	0.0	0.0	0.0	9.456410555547453	0.0
7	0.0	0.0	0.0	10.681585707053767	0.0
8	0.0	0.0	0.0	12.108213735877987	0.0
9	0.0	0.0	0.0	12.42549680946141	0.0
10	2.0832769112503203E-4	0.0	0.0	18.035761531458522	0.0
11	2.0832769112503203E-4	0.0	0.0	19.23093749544283	0.0
12	2.0832769112503203E-4	0.0	0.0	26.083668567309637	0.0
13	2.0832769112503203E-4	0.0	0.0	26.72635949443036	0.0
14	2.0832769112503203E-4	0.0	0.0	27.05885048946591	0.0
15	2.0832769112503203E-4	0.0	0.0	28.336315891444606	0.0
16	2.0832769112503203E-4	0.0	0.0	29.08587892411247	0.0
17	2.0832769112503203E-4	0.0	0.0	29.677946222289812	0.0
18	2.0832769112503203E-4	0.0	0.0	30.194390568588766	0.0
19	2.0832769112503203E-4	0.0	0.0	32.57432611200113	0.0
20	2.0832769112503203E-4	0.0	0.0	33.29347330176474	0.0
21	2.0832769112503203E-4	0.0	0.0	33.858041344713584	0.0
22	2.0832769112503203E-4	0.0	0.0	34.55739740382031	0.0
23	2.0832769112503203E-4	0.0	0.0	35.14175657742603	0.0
24	2.0832769112503203E-4	0.0	0.0	35.60028582559222	0.0
25	2.0832769112503203E-4	0.0	0.0	36.02277438319379	0.0
26	2.0832769112503203E-4	0.0	0.0	36.41776368556685	0.0
27	2.0832769112503203E-4	0.0	0.0	36.828377564774286	0.0
28	2.0832769112503203E-4	0.0	0.0	37.20690897954847	0.0
29	2.0832769112503203E-4	0.0	0.0	37.60356490345053	0.0
30	2.0832769112503203E-4	0.0	0.0	38.060010874705476	0.0
31	2.0832769112503203E-4	0.0	0.0	38.45166693402054	0.0
32	2.0832769112503203E-4	0.0	0.0	38.83540654107284	0.0
33	2.0832769112503203E-4	0.0	0.0	39.21956280350741	0.0
34	2.0832769112503203E-4	0.0	0.0	39.612260501278094	0.0
35	2.0832769112503203E-4	0.0	0.0	40.008291442106774	0.0
36	2.0832769112503203E-4	0.0	0.0	40.436196519677594	0.0
37	2.0832769112503203E-4	0.0	0.0	40.87785122486266	0.0
38	2.0832769112503203E-4	0.0	0.0	41.29867316093522	0.0
39	2.0832769112503203E-4	0.0	0.0	41.743244453796045	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGGC	40	6.8012014E-9	45.000004	28
ATTCGAT	20	7.029176E-4	45.000004	24
TCTAGCG	20	7.029176E-4	45.000004	1
TCGTGCG	35	1.2099554E-7	45.000004	1
TGATTCG	35	1.2099554E-7	45.000004	26
GTGTATC	20	7.029176E-4	45.000004	23
GATTATC	20	7.029176E-4	45.000004	9
TATGTCG	20	7.029176E-4	45.000004	1
TCGCGCG	20	7.029176E-4	45.000004	1
ATTGGGT	25	3.8873994E-5	45.0	4
CCTTGCG	25	3.8873994E-5	45.0	1
CGGGTAT	60	0.0	44.999996	6
TGGTTCG	30	2.1628512E-6	44.999996	1
CAATCAT	30	2.1628512E-6	44.999996	45
TACGGCT	2920	0.0	43.998287	7
TGATACC	2430	0.0	43.51852	4
GATACCT	2425	0.0	43.515465	5
ACGGCTG	2980	0.0	43.11242	8
GACGAAT	85	0.0	42.35294	20
ATACCTG	2500	0.0	42.3	6
>>END_MODULE