SRR3549583_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549583_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	482517
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC	35420	7.340674007340674	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG	28545	5.9158537419406985	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC	22963	4.759003309727947	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCAGAACCT	11256	2.3327675501588545	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	9117	1.8894671068584112	RNA PCR Primer, Index 40 (96% over 27bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTA	7179	1.4878232269536618	No Hit
GAACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCT	6829	1.4152869225332994	RNA PCR Primer, Index 40 (96% over 25bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTC	5605	1.1616171036460892	RNA PCR Primer, Index 40 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCT	5167	1.0708430998286071	No Hit
CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT	3316	0.687229672736919	Illumina PCR Primer Index 8 (95% over 24bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGT	2529	0.5241266110831329	No Hit
CCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	2480	0.5139715284642821	Illumina PCR Primer Index 8 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATG	2316	0.4799830886787409	No Hit
TGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCTT	1730	0.3585365904206484	RNA PCR Primer, Index 40 (96% over 29bp)
TCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	1677	0.3475525214655649	RNA PCR Primer, Index 40 (96% over 27bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTAT	1658	0.3436148363684595	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTT	1434	0.29719160153942764	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGCACCTCGTATGCCGTCTTCTGC	1347	0.2791611487263661	RNA PCR Primer, Index 43 (96% over 25bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCAGAACC	1313	0.27211476486838804	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1184	0.24537995552488304	No Hit
CGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTG	880	0.18237699397119686	Illumina PCR Primer Index 8 (95% over 22bp)
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCTCAGAACCTCG	673	0.13947695107115396	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC	665	0.13781897839868856	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC	599	0.12414070385084877	No Hit
TGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTG	554	0.11481460756823075	RNA PCR Primer, Index 40 (96% over 26bp)
TCCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTG	546	0.11315663489576533	Illumina PCR Primer Index 8 (95% over 22bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGG	35	1.2099736E-7	45.000004	2
GCTAGCG	35	1.2099736E-7	45.000004	1
GGGCAAC	35	1.2099736E-7	45.000004	7
AGGGCGA	35	1.2099736E-7	45.000004	6
CGGCGAT	45	3.8380676E-10	45.0	31
ACCGGTG	105	0.0	45.0	17
AAATCGA	25	3.8874296E-5	45.0	26
CATATAG	20	7.029214E-4	45.0	13
CGTATGG	20	7.029214E-4	45.0	2
ACGCACC	20	7.029214E-4	45.0	33
GTGGGCG	25	3.8874296E-5	45.0	5
GACAACC	20	7.029214E-4	45.0	9
AGGCGAC	50	2.1827873E-11	45.0	31
CCGTCAG	20	7.029214E-4	45.0	30
TAGCACG	20	7.029214E-4	45.0	29
ACGATAC	20	7.029214E-4	45.0	3
TACGCGG	25	3.8874296E-5	45.0	2
CTACGGC	165	0.0	45.0	6
TGCGACC	20	7.029214E-4	45.0	32
GTGTAGG	25	3.8874296E-5	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content