##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549583_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	482517
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.63017054321402	28.0	16.0	31.0	16.0	34.0
2	25.42636632491705	28.0	16.0	31.0	16.0	34.0
3	26.518723692636737	30.0	16.0	33.0	16.0	34.0
4	31.01011363330204	33.0	28.0	35.0	19.0	37.0
5	33.228607489477056	35.0	32.0	35.0	28.0	37.0
6	31.434432361968593	35.0	28.0	36.0	17.0	37.0
7	33.12962444846503	35.0	32.0	35.0	28.0	37.0
8	31.95451351973091	35.0	31.0	37.0	17.0	37.0
9	34.171305881450806	35.0	32.0	38.0	28.0	39.0
10	33.509766495273745	35.0	32.0	37.0	27.0	39.0
11	34.22891006948978	35.0	32.0	39.0	27.0	39.0
12	34.42865639967089	37.0	33.0	39.0	27.0	39.0
13	34.63074461625186	35.0	33.0	38.0	29.0	39.0
14	34.577590012372625	37.0	33.0	39.0	27.0	40.0
15	35.11983411983412	37.0	33.0	39.0	27.0	40.0
16	34.84136102976683	36.0	32.0	39.0	27.0	40.0
17	34.12360808012982	36.0	32.0	38.0	27.0	40.0
18	34.47277090755352	36.0	32.0	39.0	27.0	40.0
19	34.27697262479871	36.0	32.0	39.0	27.0	40.0
20	34.02281163150728	36.0	32.0	39.0	25.0	40.0
21	33.97187871100915	36.0	32.0	39.0	25.0	40.0
22	35.25258384678675	37.0	34.0	39.0	29.0	40.0
23	35.56963174354479	37.0	34.0	39.0	29.0	40.0
24	35.913963653094086	38.0	34.0	39.0	30.0	40.0
25	33.90325107716412	36.0	32.0	39.0	24.0	40.0
26	34.78993278993279	37.0	33.0	39.0	27.0	40.0
27	34.9957763146169	37.0	33.0	39.0	27.0	40.0
28	35.085333780985955	37.0	33.0	39.0	27.0	40.0
29	35.65600382991688	38.0	34.0	40.0	29.0	40.0
30	33.3426055455041	36.0	31.0	39.0	23.0	40.0
31	34.50560083893417	37.0	33.0	39.0	25.0	40.0
32	34.81000462159882	37.0	33.0	39.0	27.0	40.0
33	35.08772333410015	37.0	34.0	39.0	27.0	40.0
34	35.29401036647413	38.0	34.0	40.0	27.0	40.0
35	35.58649332562376	38.0	34.0	40.0	29.0	41.0
36	35.620771910626985	38.0	35.0	40.0	29.0	41.0
37	36.10999197955719	38.0	35.0	40.0	30.0	41.0
38	35.32092755281161	38.0	34.0	40.0	29.0	41.0
39	35.418391476362494	38.0	34.0	40.0	29.0	40.0
40	34.87094755210697	37.0	33.0	40.0	26.0	40.0
41	34.35986504102446	37.0	33.0	39.0	25.0	40.0
42	35.398597355119094	38.0	34.0	40.0	30.0	40.0
43	35.64128932244874	38.0	35.0	40.0	30.0	40.0
44	36.185790345210634	38.0	35.0	40.0	31.0	41.0
45	36.410462222056424	39.0	35.0	40.0	31.0	41.0
46	36.092968745142656	38.0	35.0	40.0	31.0	41.0
47	35.618108792021836	38.0	35.0	40.0	29.0	41.0
48	35.898741391495015	38.0	35.0	40.0	30.0	41.0
49	36.29327256863489	38.0	35.0	40.0	31.0	41.0
50	35.92136028367912	38.0	35.0	40.0	30.0	41.0
51	35.520412752296814	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	4.0
12	9.0
13	4.0
14	10.0
15	29.0
16	63.0
17	190.0
18	522.0
19	1056.0
20	1699.0
21	2146.0
22	2389.0
23	2577.0
24	3093.0
25	4074.0
26	5591.0
27	7367.0
28	10198.0
29	13567.0
30	18907.0
31	26382.0
32	35805.0
33	47091.0
34	57711.0
35	67970.0
36	70482.0
37	62836.0
38	36648.0
39	4092.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.02790989747511	2.9934696601363266	35.50216883550217	12.476451606886389
2	14.0998140998141	35.63853708781245	34.19692984910376	16.06471896326969
3	13.508125102328	35.52351523366016	36.496952438981424	14.471407225030413
4	14.412134701989773	2.7808346648926356	64.57119645525444	18.235834177863165
5	38.383932586831136	3.4709657898063697	36.53943798871335	21.60566363464914
6	15.33293127496026	29.206017611814712	39.8931022119428	15.567948901282236
7	55.737103563190516	1.5143507897131085	37.448007013224405	5.300538633871967
8	51.08379601133224	24.7000623812218	14.13732573152863	10.078815875917325
9	48.363270102400534	3.6550007564500318	13.864381980324008	34.117347160825425
10	39.92170224054282	21.638408594930333	21.206921206921205	17.232967957605638
11	32.78972554334873	18.951456632616054	29.248088668378525	19.01072915565669
12	21.86762331689868	14.947038135443933	31.880327532501447	31.30501101515594
13	22.458483328048544	20.27824926375651	43.04221405670681	14.221053351488134
14	19.941473564661973	29.85780811867768	24.63084202214637	25.56987629451398
15	10.882932622063057	22.864479386218516	45.013543564268204	21.239044427450224
16	12.370755849016717	24.89300895097997	24.82751903041758	37.90871616958573
17	12.989179655846323	28.40438782467768	36.188155028734734	22.41827749074126
18	11.604357981169576	24.28867791186632	33.87486865747735	30.23209544948675
19	13.004723149650685	28.040877316239637	29.32642787715252	29.627971656957165
20	16.487294748164313	25.72717645181413	43.62499145107841	14.160537348943148
21	19.219633712387335	35.996659185064985	27.294582367046132	17.489124735501544
22	13.64014117637306	21.51820557617659	36.05282300934475	28.7888302381056
23	16.159637898768334	34.047090568829695	28.71422146784466	21.07905006455731
24	21.122986340377643	22.33268465152523	25.318278941467348	31.226050066629778
25	12.55603429516473	39.158205824872496	21.94637701884079	26.33938286112199
26	14.098985113477866	21.82327254791023	33.96584990787889	30.11189243073301
27	20.004683772799716	28.817015773537513	23.10550716347818	28.072793290184595
28	11.377837464793988	29.122704485023327	36.72202222926861	22.777435820914082
29	22.26429327878603	18.30629801644294	31.035383209296253	28.39402549547477
30	14.879268502456908	33.95735279793251	27.75404804390312	23.409330655707468
31	22.92665336143597	29.336582959771366	20.135663613924486	27.601100064868177
32	26.67512232729624	32.82329948996615	19.421284638675942	21.08029354406166
33	15.023304878377342	28.246880420793463	18.314587879805273	38.41522682102392
34	22.99463024100705	28.868620172968	27.209611267582286	20.92713831844267
35	15.444222690599501	20.693156925040984	21.551572276209956	42.311048108149556
36	14.383120180221628	29.84993274848347	28.309468889179033	27.457478182115864
37	19.672881991722573	21.99963939094374	29.12021752601463	29.207261091319065
38	18.017810771433957	36.51664086446695	18.355415456864733	27.11013290723436
39	27.679232027058116	29.38114097534387	24.626697090465207	18.312929907132805
40	15.753227347430245	29.14943929436683	25.55474729387773	29.542586064325192
41	15.247960175496408	33.73414822690185	20.208407164928904	30.80948443267284
42	17.61989733004226	18.098844185800708	31.09838617084994	33.182872313307094
43	21.126716778890692	19.333826580203393	23.415133560061097	36.124323080844825
44	19.65920371717473	19.707906664428403	31.010720865793328	29.622168752603535
45	16.803760282021152	19.512473135661544	26.702893369560037	36.980873212757274
46	31.6332896043041	23.540517743416295	23.66818163919613	21.158011013083478
47	11.789429180733528	21.103712408060236	44.65542146701567	22.45143694419057
48	19.947898208767775	27.46784051131877	24.00143414636168	28.58282713355177
49	17.759581527697467	15.185786200278953	39.96916170829215	27.085470563731434
50	23.661964241674386	16.118188581956698	28.70137217963305	31.51847499673587
51	18.639343277024437	16.349268523181568	26.391194507136532	38.62019369265746
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	46.0
1	144.5
2	243.0
3	1181.5
4	2120.0
5	1504.5
6	889.0
7	1008.0
8	1127.0
9	1215.0
10	1303.0
11	1365.0
12	1427.0
13	1397.0
14	1367.0
15	1284.5
16	1202.0
17	1246.0
18	1290.0
19	1315.5
20	1341.0
21	1388.0
22	1435.0
23	1782.5
24	2130.0
25	2329.0
26	2978.5
27	3429.0
28	3726.0
29	4023.0
30	4771.5
31	5520.0
32	6213.0
33	6906.0
34	7643.0
35	8380.0
36	9212.5
37	10045.0
38	11112.5
39	12180.0
40	13642.5
41	15105.0
42	16991.0
43	18877.0
44	21436.5
45	23996.0
46	87468.0
47	150940.0
48	110085.5
49	69231.0
50	60051.5
51	50872.0
52	38258.5
53	25645.0
54	20851.0
55	16057.0
56	13835.5
57	11614.0
58	10722.5
59	9831.0
60	8602.0
61	7373.0
62	6421.5
63	5470.0
64	4346.0
65	3222.0
66	2696.5
67	2171.0
68	1780.5
69	1390.0
70	1124.5
71	859.0
72	599.5
73	340.0
74	338.5
75	235.5
76	134.0
77	98.5
78	63.0
79	57.5
80	52.0
81	28.5
82	5.0
83	3.0
84	1.0
85	1.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	482517.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.721327569599783
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.81963099983936	18.730175824442508
2	11.318732308226382	5.82265642745006
3	4.336498630196198	3.3462150531719144
4	2.208075989787449	2.2717858332756493
5	1.347138428618816	1.7325094402050236
6	0.8604113088676708	1.3278552671984074
7	0.6356656310897365	1.1445114745396838
8	0.5586148061326515	1.1494651531013123
9	0.5286107255920436	1.22369126667811
>10	5.172583265434531	24.092950175903177
>50	0.1086404523970832	1.9443856723642108
>100	0.08431796305445265	4.477467264192618
>500	0.004864497868526114	0.8168338351191134
>1k	0.008918246092297875	4.3759104406789575
>5k	0.004053748223771762	7.068730280580186
>10k+	0.003242998579017409	20.474856591099062
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC	35420	7.340674007340674	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG	28545	5.9158537419406985	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC	22963	4.759003309727947	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCAGAACCT	11256	2.3327675501588545	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	9117	1.8894671068584112	RNA PCR Primer, Index 40 (96% over 27bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTA	7179	1.4878232269536618	No Hit
GAACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCT	6829	1.4152869225332994	RNA PCR Primer, Index 40 (96% over 25bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTC	5605	1.1616171036460892	RNA PCR Primer, Index 40 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCT	5167	1.0708430998286071	No Hit
CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT	3316	0.687229672736919	Illumina PCR Primer Index 8 (95% over 24bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGT	2529	0.5241266110831329	No Hit
CCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	2480	0.5139715284642821	Illumina PCR Primer Index 8 (95% over 23bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATG	2316	0.4799830886787409	No Hit
TGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCTT	1730	0.3585365904206484	RNA PCR Primer, Index 40 (96% over 29bp)
TCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	1677	0.3475525214655649	RNA PCR Primer, Index 40 (96% over 27bp)
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTAT	1658	0.3436148363684595	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTT	1434	0.29719160153942764	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGCACCTCGTATGCCGTCTTCTGC	1347	0.2791611487263661	RNA PCR Primer, Index 43 (96% over 25bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCAGAACC	1313	0.27211476486838804	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1184	0.24537995552488304	No Hit
CGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTG	880	0.18237699397119686	Illumina PCR Primer Index 8 (95% over 22bp)
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCTCAGAACCTCG	673	0.13947695107115396	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC	665	0.13781897839868856	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC	599	0.12414070385084877	No Hit
TGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTG	554	0.11481460756823075	RNA PCR Primer, Index 40 (96% over 26bp)
TCCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTG	546	0.11315663489576533	Illumina PCR Primer Index 8 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8030805132254407	0.0
2	0.0	0.0	0.0	4.203375217867971	0.0
3	0.0	0.0	0.0	5.033605033605034	0.0
4	0.0	0.0	0.0	7.713925105229453	0.0
5	0.0	0.0	0.0	14.38767960507091	0.0
6	0.0	0.0	0.0	15.487122733499545	0.0
7	0.0	0.0	0.0	17.39109710124203	0.0
8	0.0	0.0	0.0	19.67060227929793	0.0
9	0.0	0.0	0.0	20.12592302447375	0.0
10	0.0	0.0	0.0	27.38162593235057	0.0
11	0.0	0.0	0.0	29.232959667742275	0.0
12	0.0	0.0	0.0	38.949508514725906	0.0
13	0.0	0.0	0.0	39.96957620146026	0.0
14	0.0	0.0	0.0	40.5040651417463	0.0
15	0.0	0.0	0.0	42.43684678467287	0.0
16	0.0	0.0	0.0	43.49587682921016	0.0
17	0.0	0.0	0.0	44.427035731383555	0.0
18	0.0	0.0	0.0	45.16649154330314	0.0
19	0.0	0.0	0.0	48.49217747768473	0.0
20	0.0	0.0	0.0	49.4115233245668	0.0
21	0.0	0.0	0.0	50.19719512473136	0.0
22	0.0	0.0	0.0	51.142654041204764	0.0
23	0.0	0.0	0.0	51.92459540285627	0.0
24	0.0	0.0	0.0	52.53203514073079	0.0
25	0.0	0.0	0.0	53.04890812137189	0.0
26	0.0	0.0	0.0	53.61137535050579	0.0
27	0.0	0.0	0.0	54.126383111890355	0.0
28	0.0	0.0	0.0	54.595174885029955	0.0
29	0.0	0.0	0.0	55.08199711098262	0.0
30	0.0	0.0	0.0	55.61752228418895	0.0
31	0.0	0.0	0.0	56.095018413858995	0.0
32	0.0	0.0	0.0	56.5627739540783	0.0
33	0.0	0.0	0.0	57.01913093217441	0.0
34	0.0	0.0	0.0	57.48605748605748	0.0
35	0.0	0.0	0.0	57.92542024426083	0.0
36	0.0	0.0	0.0	58.35483516642937	0.0
37	0.0	0.0	0.0	58.806010979924025	0.0
38	0.0	0.0	0.0	59.259880999011436	0.0
39	0.0	0.0	0.0	59.74981192372497	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCGG	35	1.2099736E-7	45.000004	2
GCTAGCG	35	1.2099736E-7	45.000004	1
GGGCAAC	35	1.2099736E-7	45.000004	7
AGGGCGA	35	1.2099736E-7	45.000004	6
CGGCGAT	45	3.8380676E-10	45.0	31
ACCGGTG	105	0.0	45.0	17
AAATCGA	25	3.8874296E-5	45.0	26
CATATAG	20	7.029214E-4	45.0	13
CGTATGG	20	7.029214E-4	45.0	2
ACGCACC	20	7.029214E-4	45.0	33
GTGGGCG	25	3.8874296E-5	45.0	5
GACAACC	20	7.029214E-4	45.0	9
AGGCGAC	50	2.1827873E-11	45.0	31
CCGTCAG	20	7.029214E-4	45.0	30
TAGCACG	20	7.029214E-4	45.0	29
ACGATAC	20	7.029214E-4	45.0	3
TACGCGG	25	3.8874296E-5	45.0	2
CTACGGC	165	0.0	45.0	6
TGCGACC	20	7.029214E-4	45.0	32
GTGTAGG	25	3.8874296E-5	45.0	1
>>END_MODULE