SRR3549581_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549581_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	724218
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGC	46865	6.4711178125923405	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCG	38726	5.34728493354211	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTC	27195	3.7550847949098203	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATTACCGT	15021	2.074099235313124	No Hit
GCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC	13351	1.8435056847523814	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATTACCGTCGTA	8976	1.2394058142713933	No Hit
GAACTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCT	8189	1.1307368775700135	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTC	7148	0.9869956283881373	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCT	6732	0.9295543607035451	No Hit
CTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGCT	4488	0.6197029071356966	No Hit
CCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC	3608	0.4981925331875209	No Hit
TCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC	3390	0.4680910996412682	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGATTACCGTCGT	3173	0.4381277460654112	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATG	2905	0.4011223139993759	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2237	0.3088848937750788	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTAT	2122	0.29300569717957853	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGATTACCG	1782	0.246058507245056	No Hit
CGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTG	1687	0.2329409100574689	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTT	1603	0.22134219254423393	No Hit
TGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGCTT	1513	0.20891499520862503	Illumina Single End Adapter 2 (95% over 21bp)
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGC	850	0.1173679748363062	No Hit
TCCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTG	846	0.11681565495472357	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCGATTACCGTCG	804	0.1110162961981061	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGC	800	0.11046397631652347	No Hit
TGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTG	790	0.10908317661256695	No Hit
TTCCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCT	766	0.10576925732307123	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCC	754	0.10411229767832338	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	30	2.1643846E-6	45.000004	24
CTCGTAC	30	2.1643846E-6	45.000004	29
GGTCAAC	30	2.1643846E-6	45.000004	22
CCGAGTA	35	1.2111013E-7	45.000004	41
ATACGGT	30	2.1643846E-6	45.000004	6
ACCGGTC	45	3.8380676E-10	45.0	17
GGATGCG	20	7.0315565E-4	45.0	9
TACGGTG	25	3.8893722E-5	45.0	7
TACGGAG	20	7.0315565E-4	45.0	29
TACGCGG	20	7.0315565E-4	45.0	2
TATACGG	55	1.8189894E-12	45.0	27
AAGGCGA	45	3.8380676E-10	45.0	30
CGATCAC	25	3.8893722E-5	45.0	34
TCGAATA	25	3.8893722E-5	45.0	44
TGCACGG	20	7.0315565E-4	45.0	2
TGCGTAG	20	7.0315565E-4	45.0	1
ATTTCGG	25	3.8893722E-5	45.0	2
TACCGAT	20	7.0315565E-4	45.0	18
TACGGCT	5425	0.0	43.92166	7
ACGGCTG	5445	0.0	43.760334	8

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content