##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549581_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	724218
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.061406924434355	28.0	16.0	31.0	16.0	34.0
2	25.97521464531398	30.0	16.0	33.0	16.0	34.0
3	27.030678607822505	31.0	23.0	33.0	16.0	34.0
4	31.357170078622737	33.0	28.0	35.0	19.0	37.0
5	33.33958559439285	35.0	32.0	35.0	28.0	37.0
6	31.6193894656029	35.0	28.0	37.0	17.0	37.0
7	33.18708455188907	35.0	32.0	35.0	28.0	37.0
8	32.20225677903615	35.0	32.0	37.0	17.0	37.0
9	34.23075924652522	35.0	32.0	38.0	28.0	39.0
10	33.27839683631172	35.0	31.0	37.0	27.0	39.0
11	33.94055933434408	35.0	32.0	38.0	27.0	39.0
12	34.21223305689723	35.0	32.0	39.0	27.0	39.0
13	34.40197426741672	35.0	33.0	37.0	27.0	39.0
14	34.40114716839405	36.0	32.0	39.0	27.0	40.0
15	34.928894338445055	37.0	33.0	39.0	27.0	40.0
16	34.69331057775421	36.0	32.0	39.0	27.0	40.0
17	34.03126682849639	36.0	32.0	38.0	27.0	40.0
18	34.39833033699797	36.0	32.0	39.0	27.0	40.0
19	34.22818819747645	36.0	32.0	39.0	27.0	40.0
20	34.05382771485934	36.0	32.0	39.0	25.0	40.0
21	33.87643361529263	36.0	32.0	39.0	25.0	40.0
22	35.12345177833194	37.0	33.0	39.0	27.0	40.0
23	35.50181713241041	37.0	34.0	39.0	29.0	40.0
24	35.803229138187675	38.0	34.0	39.0	30.0	40.0
25	33.821425316686415	36.0	32.0	39.0	24.0	40.0
26	34.779471098481395	37.0	33.0	39.0	27.0	40.0
27	34.94132291658037	37.0	33.0	39.0	27.0	40.0
28	35.122423082552494	37.0	34.0	39.0	27.0	40.0
29	35.614875631370666	38.0	34.0	40.0	29.0	40.0
30	33.35764921612001	36.0	31.0	39.0	23.0	40.0
31	34.35305391470524	36.0	32.0	39.0	25.0	40.0
32	34.8259584821145	37.0	33.0	39.0	28.0	40.0
33	35.171357243261006	37.0	34.0	39.0	29.0	40.0
34	35.230499656180875	37.0	34.0	40.0	29.0	40.0
35	35.37838054287521	38.0	34.0	40.0	29.0	41.0
36	35.275150576207714	38.0	34.0	40.0	27.0	41.0
37	35.480925632889544	38.0	35.0	40.0	30.0	41.0
38	35.285958095490585	37.0	34.0	40.0	30.0	41.0
39	35.19261465470342	37.0	34.0	40.0	29.0	40.0
40	34.618659022559505	37.0	33.0	39.0	26.0	40.0
41	33.96716872543902	36.0	32.0	39.0	24.0	40.0
42	35.086460154262944	37.0	34.0	39.0	29.0	40.0
43	35.11527606328481	37.0	34.0	40.0	28.0	40.0
44	35.746300147193246	38.0	35.0	40.0	30.0	41.0
45	35.98460546410059	38.0	35.0	40.0	31.0	41.0
46	35.73498587441903	38.0	35.0	40.0	29.0	41.0
47	35.18041805091837	38.0	34.0	40.0	26.0	41.0
48	35.38795224642304	38.0	34.0	40.0	28.0	41.0
49	35.66732392732575	38.0	35.0	40.0	30.0	41.0
50	35.33059796911979	38.0	34.0	40.0	28.0	41.0
51	34.845734295474564	37.0	33.0	40.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	4.0
11	9.0
12	11.0
13	15.0
14	28.0
15	54.0
16	124.0
17	416.0
18	1034.0
19	2150.0
20	3082.0
21	3514.0
22	3954.0
23	4102.0
24	5031.0
25	6568.0
26	8841.0
27	12005.0
28	15713.0
29	21712.0
30	29483.0
31	40333.0
32	54843.0
33	70258.0
34	85367.0
35	99961.0
36	105674.0
37	95017.0
38	50182.0
39	4729.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.007831895920845	3.227481228028025	39.33705044613639	11.427636429914749
2	15.449905967540161	31.47726237127495	36.63703470501976	16.435796956165134
3	13.725425217268834	31.45088909692938	39.22479143020472	15.59889425559707
4	14.753568676834874	3.066065742635505	64.03431011104391	18.14605546948571
5	35.897202223639844	3.8748001292428524	39.28761781673474	20.940379830382565
6	15.720542709515643	27.07610139488386	41.74447472998462	15.458881165615878
7	58.548807127135746	1.316316357781773	34.824873173547196	5.310003341535284
8	55.54984825011253	22.200221480272514	13.078520555965193	9.171409713649757
9	52.710647898837095	3.307981850768691	13.428000961036593	30.553369289357622
10	42.12723240792137	19.01223112377765	21.55083137950175	17.30970508879923
11	33.79700587392194	20.02642850633373	27.124981704403922	19.05158391534041
12	24.044141404936084	15.32880983350317	30.231504878365357	30.39554388319539
13	24.451753477544056	19.39084640260253	40.736905186007526	15.420494933845886
14	20.901579358701387	28.778075109980694	24.937242653455176	25.383102877862747
15	12.707361595541675	22.547492605817585	43.06079108776639	21.684354710874352
16	15.096835483238472	23.45205449187952	25.354796483931636	36.096313540950376
17	14.979605588372563	28.071105661554945	35.17739133796729	21.771897412105197
18	14.299147494262776	23.921112151313555	32.88194991010994	28.89779044431373
19	15.2071613795846	28.581725392078077	28.244257944431094	27.966855283906227
20	19.087898947554464	25.452695182942154	40.793932213780934	14.665473655722447
21	21.221510650108115	34.88259060117257	26.303129720608986	17.592769028110318
22	15.242924091917073	22.298534419194223	34.54526123349599	27.913280255392714
23	18.39501365610907	32.41220185082393	28.09872165563408	21.09406283743293
24	22.75005592238801	22.580217558801355	24.251951760381544	30.41777475842909
25	15.267778486588293	37.216694420740716	21.199694014785603	26.315833077885387
26	18.305399755322295	22.34009649028331	31.06675061928867	28.287753135105724
27	20.841514571579275	30.548536490393772	23.985043177606745	24.624905760420205
28	13.8154533579668	26.514115915373548	37.30409904200116	22.366331684658487
29	25.682046013769334	20.38792186882955	28.169694760417443	25.76033735698367
30	16.25725955444355	36.00794235989716	29.688160194858455	18.046637890800838
31	22.586293077498766	21.164621702305105	26.925870387093386	29.32321483310274
32	23.831774410467567	31.87824660530393	24.71175806179907	19.578220922429434
33	19.35977840926351	25.484453576133152	21.57320033470585	33.582567679897494
34	28.472504135495114	21.074041241725556	24.71824782040767	25.735206802371664
35	18.396532535783425	26.70825635374984	22.81357270877001	32.08163840169673
36	28.24536258419426	24.725013738957056	25.4225937493959	21.607029927452786
37	15.41939029408272	28.25806594147066	35.9587858904363	20.363757874010314
38	19.112753342225684	27.902786177642643	28.77724663015832	24.20721384997335
39	21.770516612401238	22.317175215197636	32.496706792706064	23.415601379695065
40	17.199103032512312	27.988671919228743	25.748324399559248	29.063900648699704
41	23.235683178269525	23.507424560008175	21.112841713406734	32.14405054831556
42	18.804006528421	18.44555092527388	31.467872933288042	31.282569613017074
43	32.02447329395292	19.846648384878588	22.16445876794004	25.964419553228446
44	21.281575437230227	21.30905335133896	30.530862254183134	26.878508957247682
45	18.121615314725677	16.70022009947281	29.745325302602254	35.43283928319926
46	29.385626979721575	23.590272542245565	26.754651223802778	20.26944925423008
47	14.193240156969312	21.387068534612506	43.13935859092152	21.280332717496666
48	20.63328997622263	23.775575862516536	25.79361462984902	29.797519531411815
49	18.219790173676987	16.06063367660014	39.098724417233484	26.62085173248939
50	26.3312980345697	16.207826925041907	28.77931782971426	28.681557210674136
51	19.7095349742757	16.97030452156671	26.213239659881417	37.10692084427617
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	32.0
1	177.5
2	323.0
3	2104.5
4	3886.0
5	2733.0
6	1580.0
7	1621.0
8	1662.0
9	1756.5
10	1851.0
11	1988.5
12	2126.0
13	2086.5
14	2047.0
15	1957.0
16	1867.0
17	1842.5
18	1818.0
19	1774.0
20	1730.0
21	1862.5
22	1995.0
23	2176.5
24	2358.0
25	2465.5
26	3356.5
27	4140.0
28	4797.5
29	5455.0
30	6148.5
31	6842.0
32	7878.0
33	8914.0
34	10710.5
35	12507.0
36	13302.0
37	14097.0
38	15886.0
39	17675.0
40	20165.5
41	22656.0
42	26097.5
43	29539.0
44	34381.5
45	39224.0
46	122165.5
47	205107.0
48	153158.0
49	101209.0
50	90363.0
51	79517.0
52	60339.5
53	41162.0
54	34423.0
55	27684.0
56	24818.0
57	21952.0
58	19987.0
59	18022.0
60	16500.0
61	14978.0
62	12921.0
63	10864.0
64	8901.0
65	6938.0
66	5642.0
67	4346.0
68	3339.5
69	2333.0
70	1820.0
71	1307.0
72	1054.0
73	801.0
74	697.5
75	426.5
76	259.0
77	201.0
78	143.0
79	98.5
80	54.0
81	47.0
82	40.0
83	22.5
84	5.0
85	3.5
86	2.0
87	2.5
88	3.0
89	1.5
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	724218.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.610526397744742
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.70888146519879	22.8687818823645
2	11.234110431307709	6.877640678254483
3	4.05306223308371	3.7219910543253345
4	1.9922212571462496	2.4393176552809397
5	1.1822099482122486	1.8094034413713738
6	0.881271091806716	1.6185703211591285
7	0.6895752993570679	1.477578403294158
8	0.5604129452864564	1.372362820426316
9	0.49859550086682947	1.373604366697276
>10	4.024040575277725	20.862907810295635
>50	0.09146836065508651	1.9277274791804548
>100	0.07043063770335733	4.526869699786423
>500	0.004573418032685541	1.0247614486156797
>1k	0.005030759835954095	3.990969860264453
>5k	0.0018293672130742166	4.346136498944505
>10k+	0.0022867090163427705	19.76137657973936
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGC	46865	6.4711178125923405	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCG	38726	5.34728493354211	No Hit
GAATCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTC	27195	3.7550847949098203	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGATTACCGT	15021	2.074099235313124	No Hit
GCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC	13351	1.8435056847523814	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGATTACCGTCGTA	8976	1.2394058142713933	No Hit
GAACTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCT	8189	1.1307368775700135	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTC	7148	0.9869956283881373	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCT	6732	0.9295543607035451	No Hit
CTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGCT	4488	0.6197029071356966	No Hit
CCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC	3608	0.4981925331875209	No Hit
TCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGC	3390	0.4680910996412682	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGATTACCGTCGT	3173	0.4381277460654112	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATG	2905	0.4011223139993759	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2237	0.3088848937750788	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTAT	2122	0.29300569717957853	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCGATTACCG	1782	0.246058507245056	No Hit
CGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTG	1687	0.2329409100574689	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTT	1603	0.22134219254423393	No Hit
TGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTGCTT	1513	0.20891499520862503	Illumina Single End Adapter 2 (95% over 21bp)
GAATGCTACGGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGC	850	0.1173679748363062	No Hit
TCCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTG	846	0.11681565495472357	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCGATTACCGTCG	804	0.1110162961981061	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGC	800	0.11046397631652347	No Hit
TGCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCTG	790	0.10908317661256695	No Hit
TTCCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCCGTCTTCT	766	0.10576925732307123	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGATTACCGTCGTATGCC	754	0.10411229767832338	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6970276905572631	0.0
2	0.0	0.0	0.0	3.8072790237193774	0.0
3	0.0	0.0	0.0	4.66351292014283	0.0
4	0.0	0.0	0.0	6.871687806710134	0.0
5	0.0	0.0	0.0	12.475387245276975	0.0
6	0.0	0.0	0.0	13.494417426796904	0.0
7	0.0	0.0	0.0	15.259493688364554	0.0
8	0.0	0.0	0.0	17.395590830385327	0.0
9	0.0	0.0	0.0	17.88605088523069	0.0
10	0.0	0.0	0.0	24.63111935908801	0.0
11	0.0	0.0	0.0	26.682159239345058	0.0
12	1.3807997039565434E-4	0.0	0.0	35.272252277629114	0.0
13	1.3807997039565434E-4	0.0	0.0	36.27526518258315	0.0
14	1.3807997039565434E-4	0.0	0.0	36.74653212154351	0.0
15	1.3807997039565434E-4	0.0	0.0	38.4341455197192	0.0
16	1.3807997039565434E-4	0.0	0.0	39.521387206614584	0.0
17	1.3807997039565434E-4	0.0	0.0	40.542212427749654	0.0
18	2.761599407913087E-4	0.0	0.0	41.477013827328236	0.0
19	2.761599407913087E-4	0.0	0.0	44.57552836300672	0.0
20	2.761599407913087E-4	0.0	0.0	45.5738465489673	0.0
21	2.761599407913087E-4	0.0	0.0	46.56429417661533	0.0
22	4.14239911186963E-4	0.0	0.0	47.725960967553966	0.0
23	4.14239911186963E-4	0.0	0.0	48.86470648340693	0.0
24	4.14239911186963E-4	0.0	0.0	49.69139126616571	0.0
25	4.14239911186963E-4	0.0	0.0	50.38855703669337	0.0
26	4.14239911186963E-4	0.0	0.0	51.0391898571977	0.0
27	4.14239911186963E-4	0.0	0.0	51.69962635560011	0.0
28	4.14239911186963E-4	0.0	0.0	52.329547180545084	0.0
29	5.523198815826174E-4	0.0	0.0	52.98901711915473	0.0
30	5.523198815826174E-4	0.0	0.0	53.64945361755714	0.0
31	5.523198815826174E-4	0.0	0.0	54.30188147767661	0.0
32	5.523198815826174E-4	0.0	0.0	54.91827046552281	0.0
33	5.523198815826174E-4	0.0	0.0	55.500829860622076	0.0
34	5.523198815826174E-4	0.0	0.0	56.07910877663908	0.0
35	5.523198815826174E-4	0.0	0.0	56.64330353567572	0.0
36	6.903998519782717E-4	0.0	0.0	57.25209812515016	0.0
37	6.903998519782717E-4	0.0	0.0	57.83935223924288	0.0
38	6.903998519782717E-4	0.0	0.0	58.46250714563847	0.0
39	6.903998519782717E-4	0.0	0.0	59.102507808422324	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGA	30	2.1643846E-6	45.000004	24
CTCGTAC	30	2.1643846E-6	45.000004	29
GGTCAAC	30	2.1643846E-6	45.000004	22
CCGAGTA	35	1.2111013E-7	45.000004	41
ATACGGT	30	2.1643846E-6	45.000004	6
ACCGGTC	45	3.8380676E-10	45.0	17
GGATGCG	20	7.0315565E-4	45.0	9
TACGGTG	25	3.8893722E-5	45.0	7
TACGGAG	20	7.0315565E-4	45.0	29
TACGCGG	20	7.0315565E-4	45.0	2
TATACGG	55	1.8189894E-12	45.0	27
AAGGCGA	45	3.8380676E-10	45.0	30
CGATCAC	25	3.8893722E-5	45.0	34
TCGAATA	25	3.8893722E-5	45.0	44
TGCACGG	20	7.0315565E-4	45.0	2
TGCGTAG	20	7.0315565E-4	45.0	1
ATTTCGG	25	3.8893722E-5	45.0	2
TACCGAT	20	7.0315565E-4	45.0	18
TACGGCT	5425	0.0	43.92166	7
ACGGCTG	5445	0.0	43.760334	8
>>END_MODULE