FastQCFastQC Report
Sat 18 Jun 2016
SRR3549580_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549580_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1160794
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGC506944.367183152221669No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCG443093.8171286205821184No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTC346622.986059542003146No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAGGGAAT162141.396802533438319No Hit
GCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC129851.1186308681816068No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTA97550.8403730549951154No Hit
GAACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCT93240.8032432972603235No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTC81550.7025363673485562No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCT70360.6061368339257439No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46430.39998483796435885No Hit
CCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC41620.358547683740612No Hit
CTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGCT40620.34993289076270206No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGAGGGAATCGT34900.30065627492905717No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATG29490.25405024491856437No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTT28440.2450047122917589No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTAT21950.18909470586512336No Hit
TCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC21300.1834950904294819No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGAGGGAA18540.15971826181045043No Hit
CGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTG17860.15386020258547167No Hit
TGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGCTT16470.14188564034617684Illumina Single End Adapter 2 (95% over 21bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGC12980.11182001285327112No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC207.033317E-445.036
ATTGCGT207.033317E-445.010
TATTGCG302.1655214E-644.9999961
TACGGCT58350.043.9203077
GATACCT50600.043.7104725
TGATACC50750.043.581284
ACGGCTG59150.043.402378
CGTTTTT25400.042.1653561
CGGCTGT63350.041.9100239
ATACCTG53550.041.4285746
GAATCTG42200.041.4277231
GGCGACT12450.041.2048210
ACCACCT21550.040.92807414
CCACCGG5350.040.7943915
ACCGGTG4150.040.6626517
ACCGGTC501.0822987E-940.517
CACCGGT4750.040.26315716
ATGATAC201700.040.247893
TACGGGA1400.040.178574
GCGACTG12550.040.15936711
Produced by FastQC (version 0.11.5)