##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549580_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1160794
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.925435520858997	30.0	16.0	33.0	16.0	34.0
2	26.993357133134733	31.0	23.0	33.0	16.0	34.0
3	28.068954525953785	31.0	25.0	34.0	16.0	34.0
4	31.831435207280535	35.0	28.0	35.0	19.0	37.0
5	33.434780848281434	35.0	33.0	37.0	28.0	37.0
6	32.24185169806184	35.0	32.0	37.0	17.0	37.0
7	33.15196236369244	35.0	32.0	35.0	28.0	37.0
8	32.62991969289986	35.0	32.0	37.0	25.0	37.0
9	34.36669555493912	35.0	32.0	39.0	28.0	39.0
10	33.23732031695546	35.0	31.0	37.0	27.0	39.0
11	33.735593050963395	35.0	32.0	38.0	27.0	39.0
12	33.965496031164875	35.0	32.0	39.0	26.0	39.0
13	34.19973483667214	35.0	33.0	38.0	27.0	39.0
14	34.33326326635045	37.0	32.0	39.0	25.0	40.0
15	34.74868322889333	37.0	32.0	39.0	27.0	40.0
16	34.64130328034087	36.0	32.0	39.0	27.0	40.0
17	34.09431992239794	36.0	32.0	38.0	26.0	40.0
18	34.3625707920613	36.0	32.0	39.0	27.0	40.0
19	34.12844139442485	36.0	32.0	39.0	26.0	40.0
20	34.166578221458764	36.0	32.0	39.0	25.0	40.0
21	33.86572122185332	36.0	32.0	39.0	25.0	40.0
22	34.83995782197358	37.0	33.0	39.0	27.0	40.0
23	35.49747500417818	37.0	34.0	39.0	29.0	40.0
24	35.80741716445812	38.0	34.0	40.0	30.0	40.0
25	33.85573409235403	36.0	32.0	39.0	24.0	40.0
26	34.55407936291883	36.0	33.0	39.0	27.0	40.0
27	34.8869041363067	37.0	33.0	39.0	27.0	40.0
28	35.0963280306411	37.0	34.0	39.0	27.0	40.0
29	35.66860011337067	38.0	34.0	40.0	29.0	40.0
30	33.625705336175066	36.0	32.0	39.0	23.0	40.0
31	34.456267864926936	36.0	33.0	39.0	25.0	40.0
32	34.52829442605665	37.0	33.0	39.0	26.0	40.0
33	35.2757586617436	37.0	34.0	39.0	28.0	40.0
34	35.372807750556944	38.0	34.0	40.0	29.0	41.0
35	35.541769685232694	38.0	34.0	40.0	29.0	41.0
36	35.527718096406424	38.0	34.0	40.0	28.0	41.0
37	35.68160672780873	38.0	35.0	40.0	30.0	41.0
38	35.18611054157758	38.0	34.0	40.0	28.0	41.0
39	35.10556222723412	38.0	34.0	40.0	27.0	41.0
40	34.75075422512521	37.0	33.0	40.0	26.0	41.0
41	34.28738777078448	37.0	33.0	40.0	24.0	41.0
42	34.89944727488254	37.0	34.0	40.0	27.0	41.0
43	35.20349950120349	38.0	34.0	40.0	29.0	41.0
44	35.729057007531054	38.0	35.0	40.0	30.0	41.0
45	35.86308767963997	38.0	35.0	40.0	30.0	41.0
46	35.665659023048015	38.0	35.0	40.0	30.0	41.0
47	35.26395984128106	37.0	34.0	40.0	28.0	41.0
48	35.4665780491629	37.0	34.0	40.0	29.0	41.0
49	35.61849906184904	38.0	35.0	40.0	29.0	41.0
50	35.47413839148032	37.0	34.0	40.0	29.0	41.0
51	34.86781978542274	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	11.0
10	23.0
11	27.0
12	41.0
13	29.0
14	62.0
15	128.0
16	329.0
17	1066.0
18	2172.0
19	4040.0
20	5502.0
21	6659.0
22	7245.0
23	7776.0
24	8927.0
25	11381.0
26	14864.0
27	19088.0
28	24944.0
29	32952.0
30	45224.0
31	61609.0
32	83279.0
33	104128.0
34	121672.0
35	145952.0
36	166692.0
37	167841.0
38	105159.0
39	11968.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.076533820815754	3.2324426211713706	41.0499192793898	15.64110427862308
2	17.026535285330556	24.45860333530325	41.03156977034685	17.483291609019343
3	16.581925819740626	24.44904091509777	42.55044392028215	16.418589344879454
4	15.761194492735145	3.36450739752273	60.621350558324735	20.252947551417392
5	32.27385737693337	4.236927482395671	41.27493767197281	22.21427746869815
6	18.116306597036168	21.577558119700825	43.95534435911971	16.3507909241433
7	66.7126983771453	1.6128615413243004	27.46843970592543	4.206000375604973
8	65.06012264019283	17.30858360742733	11.070008976614282	6.561284775765554
9	61.303900605964536	3.744850507497454	11.166236214177538	23.78501267236047
10	43.5296012901514	21.64492580078808	18.782402390088162	16.043070518972357
11	34.83408770203843	19.81006104442304	26.425102128370753	18.93074912516777
12	26.217141025884004	16.53850726313196	29.513677706811027	27.730674004173007
13	26.543641679746795	18.380263853879327	37.43110319315917	17.644991273214714
14	20.6387179809682	26.459819744071726	28.508589810078274	24.392872464881794
15	15.144633759306132	21.380710100155582	40.916131544442855	22.55852459609543
16	19.345723702913695	23.170605637175935	25.77597747748524	31.707693182425135
17	19.956081785398617	26.62918657401744	32.617156877103085	20.79757476348086
18	19.255440672505202	22.896827516337957	31.025143134785328	26.822588676371517
19	19.14146696140745	26.36333406271914	27.494197936929375	27.001001038944032
20	22.187571610466627	25.73178358950856	36.32125941381503	15.759385386209784
21	23.031821322301806	31.38188171200058	28.065617155154143	17.520679810543474
22	19.154130707084978	19.958321631572872	35.36975552940487	25.517792131937277
23	21.305847549177546	28.5690656567832	30.066575120133287	20.058511673905965
24	22.742795017892924	23.639594966893352	26.50573659064399	27.111873424569733
25	18.812726461370406	33.21855557489098	23.66647312098443	24.302244842754185
26	18.476232647653244	21.89001666100962	32.34346490419489	27.29028578714225
27	23.906998140927676	25.882973206270883	26.073015539363574	24.13701311343787
28	15.733541007276052	27.431223800260856	33.13783496468796	23.697400227775127
29	24.74099624911914	20.66998967947801	30.29142121685674	24.297592854546114
30	25.180953726501	27.444404433517057	28.6993213266092	18.675320513372743
31	26.687594870407672	24.537687134840464	24.055689467726403	24.719028527025465
32	32.076750913598794	27.964910225242377	22.688694117991652	17.269644743167177
33	27.869975206625806	22.303612871879075	22.04887344352228	27.777538477972836
34	28.024955332298408	23.352033177290714	29.174082567621817	19.448928922789058
35	25.994707071194373	26.93923297329242	24.265890416387403	22.800169539125807
36	21.857452743553118	32.322014069679895	26.434319956857117	19.38621322990987
37	29.541158896410558	24.283378446132563	27.43880481808142	18.73665783937546
38	26.592401408001763	29.5294427779606	21.13880671333587	22.739349100701762
39	32.26886079700619	23.63864733966578	24.40501932298065	19.687472540347382
40	27.956209284334687	24.531915223545266	26.046051237342716	21.46582425477733
41	25.413811580693906	28.99411954231328	22.465054092285108	23.127014784707708
42	20.533014471129242	27.061304589789405	30.1188669135092	22.286814025572152
43	23.251670839098065	26.472225045959917	25.89244947854658	24.383654636395434
44	26.277272280869816	19.762765830974317	29.72904753125878	24.23091435689709
45	21.346940111682176	20.730810117902056	27.238941620993906	30.683308149421862
46	29.60068711588792	25.089033885426698	24.888739948690294	20.42153904999509
47	18.904560154514925	21.92912782112933	38.36451601231571	20.801796012040036
48	24.29302701426782	25.29785646721124	25.830767560824746	24.578348957696196
49	22.2783715284538	20.753036283785065	34.660068883884655	22.308523303876484
50	24.409240571539826	21.671114771440926	28.162533576155628	25.757111080863616
51	21.54585568154212	20.003032407128224	27.321212893932945	31.12989901739671
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	117.0
1	543.0
2	969.0
3	4781.0
4	8593.0
5	5934.0
6	3275.0
7	3185.0
8	3095.0
9	3027.5
10	2960.0
11	2944.0
12	2928.0
13	2759.0
14	2590.0
15	2491.5
16	2393.0
17	2452.5
18	2512.0
19	2600.5
20	2689.0
21	2950.0
22	3211.0
23	3584.5
24	3958.0
25	4745.5
26	6798.5
27	8064.0
28	9281.0
29	10498.0
30	12002.5
31	13507.0
32	16100.5
33	18694.0
34	21056.5
35	23419.0
36	24978.0
37	26537.0
38	29626.0
39	32715.0
40	35029.5
41	37344.0
42	41202.0
43	45060.0
44	50641.5
45	56223.0
46	149971.0
47	243719.0
48	184783.0
49	125847.0
50	117018.5
51	108190.0
52	90951.0
53	73712.0
54	67725.5
55	61739.0
56	57247.0
57	52755.0
58	50529.5
59	48304.0
60	44334.0
61	40364.0
62	34843.0
63	29322.0
64	25509.0
65	21696.0
66	17916.0
67	14136.0
68	12218.0
69	10300.0
70	8094.0
71	5888.0
72	4705.5
73	3523.0
74	2886.0
75	1655.0
76	1061.0
77	857.0
78	653.0
79	459.5
80	266.0
81	205.0
82	144.0
83	90.0
84	36.0
85	19.5
86	3.0
87	2.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1160794.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.651337202617622
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.26028785484841	21.276565480927122
2	9.711153459538235	5.564750647911933
3	3.366166084916237	2.8933547873685086
4	1.714609438317934	1.9650341275215168
5	1.0865994670706354	1.5566263867612686
6	0.8600703649383951	1.4785299626297013
7	0.714157083610903	1.4323088792721776
8	0.6459282424187972	1.480536630578808
9	0.6105291961617977	1.5743230084147268
>10	6.8826807090618045	35.69810896002066
>50	0.09112919153339658	1.769872105871823
>100	0.044802523735536996	2.507749953211742
>500	0.005486023314454736	1.0502370877840221
>1k	0.0036573488763031576	2.88690763466698
>5k	0.0012191162921010525	2.9925688033889113
>10k+	0.0015238953651263158	13.87252554367015
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGC	50694	4.367183152221669	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCG	44309	3.8171286205821184	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTC	34662	2.986059542003146	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGAGGGAAT	16214	1.396802533438319	No Hit
GCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	12985	1.1186308681816068	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTA	9755	0.8403730549951154	No Hit
GAACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCT	9324	0.8032432972603235	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTC	8155	0.7025363673485562	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCT	7036	0.6061368339257439	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4643	0.39998483796435885	No Hit
CCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	4162	0.358547683740612	No Hit
CTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGCT	4062	0.34993289076270206	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGAGGGAATCGT	3490	0.30065627492905717	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATG	2949	0.25405024491856437	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTT	2844	0.2450047122917589	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTAT	2195	0.18909470586512336	No Hit
TCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	2130	0.1834950904294819	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGAGGGAA	1854	0.15971826181045043	No Hit
CGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTG	1786	0.15386020258547167	No Hit
TGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGCTT	1647	0.14188564034617684	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGC	1298	0.11182001285327112	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3837028792361091	0.0
2	0.0	0.0	0.0	2.1688602801186083	0.0
3	0.0	0.0	0.0	2.6406063435889573	0.0
4	0.0	0.0	0.0	4.050934102002595	0.0
5	0.0	0.0	0.0	8.061378677009014	0.0
6	0.0	0.0	0.0	8.811382553665853	0.0
7	0.0	0.0	0.0	9.962663487233739	0.0
8	0.0	0.0	0.0	11.355761659691556	0.0
9	0.0	0.0	0.0	11.716721485465984	0.0
10	0.0	0.0	0.0	16.424361256174652	0.0
11	0.0	0.0	0.0	17.61285809540711	0.0
12	0.0	0.0	0.0	23.43111697682793	0.0
13	0.0	0.0	0.0	24.065941071370116	0.0
14	0.0	0.0	0.0	24.404674731261533	0.0
15	0.0	0.0	0.0	25.55207900798936	0.0
16	8.614792977909948E-5	0.0	0.0	26.234370611839825	0.0
17	8.614792977909948E-5	0.0	0.0	26.841971960571815	0.0
18	8.614792977909948E-5	0.0	0.0	27.370144918047476	0.0
19	8.614792977909948E-5	0.0	0.0	29.39867022055593	0.0
20	8.614792977909948E-5	0.0	0.0	30.064766013607926	0.0
21	8.614792977909948E-5	0.0	0.0	30.685634143525895	0.0
22	8.614792977909948E-5	0.0	0.0	31.456744262978617	0.0
23	8.614792977909948E-5	0.0	0.0	32.08174749352598	0.0
24	1.7229585955819897E-4	0.0	0.0	32.60759445689761	0.0
25	1.7229585955819897E-4	0.0	0.0	33.07408549665143	0.0
26	1.7229585955819897E-4	0.0	0.0	33.53807824644166	0.0
27	1.7229585955819897E-4	0.0	0.0	34.03075825684833	0.0
28	1.7229585955819897E-4	0.0	0.0	34.45124630210012	0.0
29	1.7229585955819897E-4	0.0	0.0	34.92729976205942	0.0
30	1.7229585955819897E-4	0.0	0.0	35.4681364652126	0.0
31	2.5844378933729845E-4	0.0	0.0	35.91843169416796	0.0
32	2.5844378933729845E-4	0.0	0.0	36.3452946862234	0.0
33	2.5844378933729845E-4	0.0	0.0	36.794211548302286	0.0
34	2.5844378933729845E-4	0.0	0.0	37.25269083058665	0.0
35	2.5844378933729845E-4	0.0	0.0	37.74890290611426	0.0
36	2.5844378933729845E-4	0.0	0.0	38.19704443682514	0.0
37	2.5844378933729845E-4	0.0	0.0	38.64225693792353	0.0
38	2.5844378933729845E-4	0.0	0.0	39.179130836306875	0.0
39	2.5844378933729845E-4	0.0	0.0	40.000895938469704	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGAAC	20	7.033317E-4	45.0	36
ATTGCGT	20	7.033317E-4	45.0	10
TATTGCG	30	2.1655214E-6	44.999996	1
TACGGCT	5835	0.0	43.920307	7
GATACCT	5060	0.0	43.710472	5
TGATACC	5075	0.0	43.58128	4
ACGGCTG	5915	0.0	43.40237	8
CGTTTTT	2540	0.0	42.165356	1
CGGCTGT	6335	0.0	41.910023	9
ATACCTG	5355	0.0	41.428574	6
GAATCTG	4220	0.0	41.427723	1
GGCGACT	1245	0.0	41.20482	10
ACCACCT	2155	0.0	40.928074	14
CCACCGG	535	0.0	40.79439	15
ACCGGTG	415	0.0	40.66265	17
ACCGGTC	50	1.0822987E-9	40.5	17
CACCGGT	475	0.0	40.263157	16
ATGATAC	20170	0.0	40.24789	3
TACGGGA	140	0.0	40.17857	4
GCGACTG	1255	0.0	40.159367	11
>>END_MODULE