SRR3549578_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549578_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	302807
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGC	24208	7.994531170019187	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCG	20714	6.840660883004686	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC	15683	5.179206557312083	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGGCGAT	8048	2.6577985317380377	No Hit
GCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	7860	2.595712780748133	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGGCGATCGTA	4974	1.642630454381834	No Hit
GAACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT	4734	1.563372048862807	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTC	3988	1.317010505041165	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCT	3807	1.2572364575455652	No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT	2662	0.8791078145485407	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	2566	0.84740445234093	No Hit
TCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	1991	0.6575145224515946	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAATGGCGATCGT	1775	0.5861819574844703	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATG	1550	0.5118772023103825	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTAT	1183	0.39067789053753715	No Hit
TGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCTT	1083	0.3576535549046092	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAATGGCGA	985	0.32528970598433987	No Hit
CGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	944	0.3117497283748394	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTT	862	0.2846697731558385	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	729	0.2407474067640444	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCAATGGCGATCG	498	0.164461191451981	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGC	475	0.15686559425640753	No Hit
TCCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	434	0.1433256166469071	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTGGCGATCGTATGCCGTCTTC	426	0.14068366979627286	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGC	411	0.1357300194513337	No Hit
TGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	406	0.1340788026696873	No Hit
TTCCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT	406	0.1340788026696873	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCC	333	0.10997103765764991	No Hit
GAATGATACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGT	331	0.10931055094499137	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCG	322	0.10633836073802784	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	50	2.1827873E-11	45.000004	31
CTCGTTG	25	3.883978E-5	45.000004	1
AGTTTGG	25	3.883978E-5	45.000004	2
GTTTACA	35	1.2079727E-7	45.000004	41
GGGTAAT	25	3.883978E-5	45.000004	7
AGGCGAC	25	3.883978E-5	45.000004	31
ACTACAC	25	3.883978E-5	45.000004	24
CGGGACA	50	2.1827873E-11	45.000004	6
AACTCAA	25	3.883978E-5	45.000004	38
GGACCCA	25	3.883978E-5	45.000004	8
CACCGAG	25	3.883978E-5	45.000004	16
TCAAGTA	25	3.883978E-5	45.000004	41
CTTCGTG	20	7.025049E-4	45.0	1
TGTCACG	20	7.025049E-4	45.0	15
TACTTTT	20	7.025049E-4	45.0	11
GTATTAC	20	7.025049E-4	45.0	45
ATAGGGC	20	7.025049E-4	45.0	4
CGACGAA	20	7.025049E-4	45.0	19
AGAAGTG	75	0.0	45.0	3
CGGCAAG	20	7.025049E-4	45.0	34

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content