##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549578_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	302807
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	24.110007364426846	25.0	16.0	31.0	16.0	33.0
2	24.832566618341055	28.0	16.0	31.0	16.0	34.0
3	25.900474559703046	30.0	16.0	33.0	16.0	34.0
4	30.612452816480463	32.0	28.0	35.0	19.0	37.0
5	33.10023876594663	35.0	32.0	35.0	28.0	37.0
6	31.02252589933522	35.0	28.0	36.0	17.0	37.0
7	33.071375496603444	35.0	32.0	35.0	28.0	37.0
8	31.556199163163335	35.0	28.0	36.0	17.0	37.0
9	34.116985406546085	35.0	32.0	38.0	28.0	39.0
10	33.580610752063194	35.0	32.0	37.0	28.0	39.0
11	34.25751716439844	35.0	32.0	39.0	27.0	39.0
12	34.42960037251451	35.0	34.0	39.0	27.0	39.0
13	34.596052931405154	35.0	33.0	38.0	28.0	39.0
14	34.49599910173807	36.0	33.0	38.0	27.0	40.0
15	35.06307317862533	37.0	33.0	39.0	27.0	40.0
16	34.60403821576119	36.0	32.0	39.0	27.0	40.0
17	34.063548729058446	36.0	32.0	38.0	27.0	40.0
18	34.426681021244555	36.0	32.0	38.0	27.0	40.0
19	34.11190626372574	36.0	32.0	39.0	27.0	40.0
20	33.80835978032212	35.0	31.0	39.0	25.0	40.0
21	33.84863626006004	36.0	32.0	39.0	25.0	40.0
22	35.18132341722615	37.0	33.0	39.0	28.0	40.0
23	35.40935315233796	37.0	33.0	39.0	29.0	40.0
24	35.76435485309124	37.0	34.0	39.0	30.0	40.0
25	33.73716261513109	36.0	32.0	39.0	24.0	40.0
26	34.81915213320696	37.0	33.0	39.0	27.0	40.0
27	34.8567371295908	37.0	33.0	39.0	27.0	40.0
28	34.93746842047905	37.0	33.0	39.0	27.0	40.0
29	35.35920239624579	37.0	34.0	40.0	27.0	40.0
30	33.31708646101312	36.0	31.0	39.0	23.0	40.0
31	34.31740679706876	36.0	32.0	39.0	25.0	40.0
32	34.59880385856338	37.0	33.0	39.0	27.0	40.0
33	35.049477059645255	37.0	34.0	39.0	27.0	40.0
34	35.198377184147	38.0	34.0	40.0	27.0	40.0
35	35.366675803399524	38.0	34.0	40.0	27.0	40.0
36	35.470359007552666	38.0	34.0	40.0	28.0	41.0
37	35.54696555892037	38.0	35.0	40.0	30.0	41.0
38	35.3457152575733	38.0	34.0	40.0	30.0	41.0
39	35.04548441746723	37.0	34.0	40.0	27.0	40.0
40	34.81800288632694	37.0	33.0	40.0	26.0	40.0
41	34.1967127576311	37.0	33.0	39.0	25.0	40.0
42	35.211649664637875	37.0	34.0	40.0	30.0	40.0
43	35.361854250397116	38.0	35.0	40.0	29.0	40.0
44	35.90167004065296	38.0	35.0	40.0	30.0	41.0
45	36.1384049906376	38.0	35.0	40.0	31.0	41.0
46	35.73590769037704	38.0	35.0	40.0	29.0	41.0
47	35.01362584088215	37.0	33.0	40.0	26.0	41.0
48	35.46710611049282	38.0	34.0	40.0	28.0	41.0
49	35.8979680126285	38.0	35.0	40.0	30.0	41.0
50	35.54353763288167	38.0	34.0	40.0	28.0	41.0
51	35.002674971186266	37.0	34.0	40.0	27.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	2.0
11	4.0
12	4.0
13	9.0
14	9.0
15	15.0
16	45.0
17	139.0
18	342.0
19	623.0
20	1071.0
21	1465.0
22	1688.0
23	1860.0
24	2116.0
25	2825.0
26	3856.0
27	5067.0
28	6906.0
29	9329.0
30	12624.0
31	17032.0
32	23984.0
33	31007.0
34	37413.0
35	43624.0
36	43167.0
37	36074.0
38	18736.0
39	1768.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.586961331805405	2.7096467386817347	31.940146694098882	12.763245235413976
2	13.859983421783511	39.35014712341525	30.10894728325303	16.68092217154821
3	12.027793280868671	39.69062802379073	34.392864101556434	13.88871459378416
4	14.432625401658484	2.8031056085229205	65.64379291099611	17.120476078822485
5	40.42244730141641	3.6541427377834723	34.28817695760005	21.635233003200057
6	14.184942884411523	32.36715135383264	37.30263831417371	16.145267447582125
7	50.517986704402475	1.2440267232923943	42.34050071497686	5.897485857328265
8	43.81206511078013	27.52446277662009	16.652521242903894	12.010950869695879
9	41.60901168070751	3.393580729639672	16.574253567453855	38.42315402219896
10	40.25006026941253	16.724844537940008	24.44296201871159	18.582133173935876
11	34.11942260251579	18.448714857978846	27.96599814403234	19.465864395473023
12	21.837341937273578	13.832242979851852	32.05441089538881	32.27600418748576
13	21.67486220595957	20.403755527448176	43.81966070797571	14.101721558616545
14	19.178882918822882	31.39524515615557	22.950922534815906	26.474949390205644
15	8.995168539696904	22.480986238759343	46.855918126067095	21.667927095476657
16	10.581657623502759	24.540383808828725	23.73194807253465	41.146010495133865
17	11.596495457502634	28.757921712509948	37.00343783333938	22.64214499664803
18	11.09452555588213	23.75143243055808	34.352244168727935	30.801797844831857
19	11.709768928723577	28.81505381315491	27.97359374122791	31.5015835168936
20	15.064050698960063	25.643396618968517	44.98046610547312	14.312086576598295
21	18.962573520427203	37.22734282893064	26.547602928598085	17.262480722044074
22	12.13016872133075	21.175204007833372	36.12003685515857	30.57459041567731
23	15.859606944357296	34.251189701691175	28.182307542428013	21.70689581152351
24	21.89084136099892	21.49620055018543	23.398402282642078	33.21455580617357
25	11.318100308117051	41.98714032370454	19.544132070923062	27.150627297255348
26	13.60536579405364	25.342214677996218	30.95139808524902	30.101021442701125
27	20.083749715165105	33.06330434897476	21.74949720448999	25.103448731370147
28	10.363697008325436	28.781699234165657	38.19627683640074	22.658326921108166
29	24.351484609008377	25.72628770140717	23.322776554042672	26.59945113554178
30	18.673940826995413	36.56421416942145	28.388049153421157	16.373795850161983
31	24.382197241147	21.21351223716757	23.738883183017563	30.665407338667865
32	34.84166482280793	33.51111434015726	16.341432001241714	15.305788835793097
33	23.4211890742288	22.513350087679612	18.38662910698894	35.67883173110265
34	27.029097742126172	27.202475504199043	21.774595699571016	23.99383105410377
35	24.96936992870046	28.95870967315815	19.020696351141154	27.051224047000233
36	17.984722942336205	37.6893532844353	24.851473050490906	19.474450722737586
37	22.72437559237402	28.773112906901094	30.590772340137445	17.911739160587437
38	29.17931223518611	22.552649047082795	26.19688448417639	22.071154233554708
39	31.203373766128262	23.76827484173087	22.934080123643113	22.094271268497756
40	34.54774823567487	19.889896864999816	24.599827612967996	20.962527286357314
41	18.883975601620833	30.74631696096854	19.093680132889926	31.276027304520703
42	29.07363436116074	17.414062422599212	31.405152456845446	22.107150759394596
43	25.365001469582936	30.213634427209414	19.271681301951407	25.149682801256247
44	21.784503000260894	20.546090414026096	30.798825654624896	26.870580931088117
45	19.297440283745093	14.598077323179451	30.18787544541573	35.91660694765973
46	35.01306112474282	24.594213475910397	23.315511200203428	17.07721419914335
47	16.20239954822709	17.4421331078872	46.38069793630927	19.974769407576442
48	22.045065008404695	22.866049992239283	23.77818214242075	31.310702856935276
49	21.938726647666666	13.109670516203389	41.566410287741036	23.385192548388908
50	26.550575118805046	16.78825126235523	29.098732856241767	27.562440762597955
51	20.373703382022214	14.869867605438447	26.131166056266864	38.62526295627248
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	56.0
1	115.5
2	175.0
3	724.5
4	1274.0
5	903.5
6	533.0
7	662.5
8	792.0
9	1003.5
10	1215.0
11	1227.0
12	1239.0
13	1144.0
14	1049.0
15	1028.0
16	1007.0
17	906.5
18	806.0
19	793.0
20	780.0
21	831.0
22	882.0
23	1010.0
24	1138.0
25	1282.5
26	1582.5
27	1738.0
28	1951.5
29	2165.0
30	2376.0
31	2587.0
32	2796.5
33	3006.0
34	3224.0
35	3442.0
36	3690.0
37	3938.0
38	4908.0
39	5878.0
40	6596.5
41	7315.0
42	8321.5
43	9328.0
44	11765.0
45	14202.0
46	58479.5
47	102757.0
48	74818.0
49	46879.0
50	41508.5
51	36138.0
52	25765.0
53	15392.0
54	12473.0
55	9554.0
56	8478.5
57	7403.0
58	6494.5
59	5586.0
60	4980.0
61	4374.0
62	3647.5
63	2921.0
64	2449.0
65	1977.0
66	1745.5
67	1514.0
68	1241.0
69	968.0
70	784.5
71	601.0
72	455.0
73	309.0
74	274.5
75	168.0
76	96.0
77	66.5
78	37.0
79	51.0
80	65.0
81	42.5
82	20.0
83	10.5
84	1.0
85	1.5
86	2.0
87	1.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	302807.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.915494027548903
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.25952737356138	19.987318655116955
2	10.791146229540379	5.808980637832018
3	3.969227749012294	3.205011773175653
4	2.1005619494000145	2.261506504142903
5	1.4061004637922996	1.8922944317667687
6	1.058869720988442	1.7100000990730069
7	0.8539667738215012	1.6089456320362474
8	0.7791219847365709	1.6776362501527375
9	0.6576525729429953	1.5930939509324422
>10	3.8956099236828545	16.60991984993742
>50	0.11410762926063163	2.15285643991057
>100	0.08956835415081839	5.051402378412653
>500	0.004907855021962651	1.1624566142790622
>1k	0.013496601310397292	10.010666860409435
>5k	0.0024539275109813257	5.253511312486172
>10k+	0.0036808912664719885	20.014398610335956
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGC	24208	7.994531170019187	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCG	20714	6.840660883004686	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC	15683	5.179206557312083	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAATGGCGAT	8048	2.6577985317380377	No Hit
GCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	7860	2.595712780748133	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAATGGCGATCGTA	4974	1.642630454381834	No Hit
GAACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT	4734	1.563372048862807	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTC	3988	1.317010505041165	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCT	3807	1.2572364575455652	No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT	2662	0.8791078145485407	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	2566	0.84740445234093	No Hit
TCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	1991	0.6575145224515946	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAATGGCGATCGT	1775	0.5861819574844703	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATG	1550	0.5118772023103825	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTAT	1183	0.39067789053753715	No Hit
TGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCTT	1083	0.3576535549046092	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCAATGGCGA	985	0.32528970598433987	No Hit
CGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	944	0.3117497283748394	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTT	862	0.2846697731558385	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	729	0.2407474067640444	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCAATGGCGATCG	498	0.164461191451981	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGC	475	0.15686559425640753	No Hit
TCCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	434	0.1433256166469071	No Hit
GAATCTGTCTCTTATACACATCTGACGCACTGGCGATCGTATGCCGTCTTC	426	0.14068366979627286	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGC	411	0.1357300194513337	No Hit
TGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTG	406	0.1340788026696873	No Hit
TTCCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT	406	0.1340788026696873	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCC	333	0.10997103765764991	No Hit
GAATGATACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGT	331	0.10931055094499137	No Hit
GAATGCTACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCG	322	0.10633836073802784	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9913905557004957	0.0
2	0.0	0.0	0.0	5.404102282972322	0.0
3	0.0	0.0	0.0	6.55037697279125	0.0
4	0.0	0.0	0.0	9.626924080354813	0.0
5	0.0	0.0	0.0	17.362874702368174	0.0
6	0.0	0.0	0.0	18.640586248006155	0.0
7	0.0	0.0	0.0	20.89317618152817	0.0
8	0.0	0.0	0.0	23.547672279702912	0.0
9	0.0	0.0	0.0	24.095546007853187	0.0
10	0.0	0.0	0.0	32.49033212574346	0.0
11	0.0	0.0	0.0	34.54378531539892	0.0
12	0.0	0.0	0.0	44.88568626220662	0.0
13	0.0	0.0	0.0	46.00157856324326	0.0
14	0.0	0.0	0.0	46.62177558642964	0.0
15	0.0	0.0	0.0	48.760101318661725	0.0
16	0.0	0.0	0.0	49.97407589652815	0.0
17	0.0	0.0	0.0	50.93607479351534	0.0
18	0.0	0.0	0.0	51.731300795556244	0.0
19	0.0	0.0	0.0	55.48583751366382	0.0
20	0.0	0.0	0.0	56.4544412777776	0.0
21	0.0	0.0	0.0	57.196167856093155	0.0
22	0.0	0.0	0.0	58.03795817137649	0.0
23	0.0	0.0	0.0	58.73080873295532	0.0
24	0.0	0.0	0.0	59.29156195200243	0.0
25	0.0	0.0	0.0	59.822263025623585	0.0
26	0.0	0.0	0.0	60.25257011892063	0.0
27	0.0	0.0	0.0	60.709957167436684	0.0
28	0.0	0.0	0.0	61.12243111949196	0.0
29	0.0	0.0	0.0	61.56892013724914	0.0
30	0.0	0.0	0.0	62.05404762769685	0.0
31	0.0	0.0	0.0	62.48864788462618	0.0
32	0.0	0.0	0.0	62.9027730534631	0.0
33	0.0	0.0	0.0	63.3202006558633	0.0
34	0.0	0.0	0.0	63.69766881214767	0.0
35	0.0	0.0	0.0	64.10155643693838	0.0
36	0.0	0.0	0.0	64.49190408411958	0.0
37	0.0	0.0	0.0	64.9014058459679	0.0
38	0.0	0.0	0.0	65.30991687774721	0.0
39	0.0	0.0	0.0	65.73824251090629	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	50	2.1827873E-11	45.000004	31
CTCGTTG	25	3.883978E-5	45.000004	1
AGTTTGG	25	3.883978E-5	45.000004	2
GTTTACA	35	1.2079727E-7	45.000004	41
GGGTAAT	25	3.883978E-5	45.000004	7
AGGCGAC	25	3.883978E-5	45.000004	31
ACTACAC	25	3.883978E-5	45.000004	24
CGGGACA	50	2.1827873E-11	45.000004	6
AACTCAA	25	3.883978E-5	45.000004	38
GGACCCA	25	3.883978E-5	45.000004	8
CACCGAG	25	3.883978E-5	45.000004	16
TCAAGTA	25	3.883978E-5	45.000004	41
CTTCGTG	20	7.025049E-4	45.0	1
TGTCACG	20	7.025049E-4	45.0	15
TACTTTT	20	7.025049E-4	45.0	11
GTATTAC	20	7.025049E-4	45.0	45
ATAGGGC	20	7.025049E-4	45.0	4
CGACGAA	20	7.025049E-4	45.0	19
AGAAGTG	75	0.0	45.0	3
CGGCAAG	20	7.025049E-4	45.0	34
>>END_MODULE