SRR3549577_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549577_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	411076
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGC	26680	6.4902840350689415	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCG	21524	5.2360147515301305	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTC	17210	4.186573772246495	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTATACGCT	8336	2.027848864930086	No Hit
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	7154	1.7403107941110645	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTATACGCTCGTA	5259	1.27932547752727	No Hit
GAACTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCT	4782	1.1632885403185786	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTC	4222	1.0270606895075363	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCT	3719	0.9046988877969037	No Hit
CTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGCT	2572	0.6256750576535726	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	2130	0.5181523611205714	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	1869	0.4546604520818535	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTATACGCTCGT	1842	0.4480923235606068	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATG	1712	0.41646800105090054	No Hit
TGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGCTT	1242	0.30213391197734724	TruSeq Adapter, Index 13 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1222	0.2972686315912386	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTAT	1182	0.2875380708190213	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTT	978	0.23791221088071304	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTATACGC	951	0.23134408235946638	No Hit
CGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTG	674	0.16395994901186153	No Hit
TCCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTG	525	0.1277136101353521	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCTATACGCTCG	483	0.11749652132452394	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGC	454	0.1104418647646664	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGC	423	0.10290068016619797	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	20	7.0279924E-4	45.000004	31
GATCGTT	20	7.0279924E-4	45.000004	9
TAGGTTG	20	7.0279924E-4	45.000004	37
GGACTAA	65	0.0	45.000004	8
TATATGA	20	7.0279924E-4	45.000004	14
ATCGTTT	20	7.0279924E-4	45.000004	10
CTGCGAT	40	6.7975634E-9	45.000004	31
GTTCGTG	20	7.0279924E-4	45.000004	1
CGTAATG	20	7.0279924E-4	45.000004	42
GCGTCGG	20	7.0279924E-4	45.000004	2
GTTATGG	20	7.0279924E-4	45.000004	2
TGCCGTG	20	7.0279924E-4	45.000004	1
TCTGCGA	25	3.8864182E-5	45.0	30
ACTGACG	25	3.8864182E-5	45.0	43
ACCGACG	25	3.8864182E-5	45.0	17
CTCTTCG	45	3.8380676E-10	45.0	1
CGCTCGG	25	3.8864182E-5	45.0	2
TTGCGCG	55	1.8189894E-12	45.0	1
GTTTTCG	25	3.8864182E-5	45.0	1
GGACCAT	25	3.8864182E-5	45.0	8

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content