##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549577_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	411076
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	25.093357432688848	28.0	16.0	31.0	16.0	34.0
2	25.915721180511632	30.0	16.0	33.0	16.0	34.0
3	26.948272338934892	31.0	16.0	33.0	16.0	34.0
4	31.1870627329253	33.0	28.0	35.0	19.0	37.0
5	33.26507507127636	35.0	32.0	35.0	28.0	37.0
6	31.567982562835095	35.0	28.0	37.0	17.0	37.0
7	33.099920695929704	35.0	32.0	35.0	28.0	37.0
8	32.14154803491325	35.0	32.0	37.0	17.0	37.0
9	34.16006529206278	35.0	32.0	38.0	28.0	39.0
10	33.45857700279267	35.0	32.0	37.0	27.0	39.0
11	34.022908172697996	35.0	32.0	39.0	27.0	39.0
12	34.260779028695424	35.0	32.0	39.0	27.0	39.0
13	34.42534957039574	35.0	33.0	38.0	27.0	39.0
14	34.32499099923128	36.0	32.0	39.0	26.0	40.0
15	34.91903200381438	37.0	33.0	39.0	27.0	40.0
16	34.66411807062441	36.0	32.0	39.0	27.0	40.0
17	34.01532563321624	36.0	32.0	38.0	27.0	40.0
18	34.31981190826027	36.0	32.0	39.0	27.0	40.0
19	34.135894579104594	36.0	32.0	39.0	27.0	40.0
20	33.991471163483155	36.0	32.0	39.0	25.0	40.0
21	33.86727028578657	36.0	32.0	39.0	25.0	40.0
22	35.03001877998229	37.0	33.0	39.0	27.0	40.0
23	35.40099640942307	37.0	33.0	39.0	29.0	40.0
24	35.704310151894056	37.0	34.0	39.0	30.0	40.0
25	33.81639891406942	36.0	32.0	39.0	24.0	40.0
26	34.665023012776224	37.0	33.0	39.0	27.0	40.0
27	34.88714495616382	37.0	33.0	39.0	27.0	40.0
28	34.8223929395051	37.0	33.0	39.0	26.0	40.0
29	35.42493602156292	38.0	34.0	40.0	27.0	40.0
30	33.35574443655188	36.0	31.0	39.0	22.0	40.0
31	34.263464663468554	36.0	32.0	39.0	25.0	40.0
32	34.60784623767868	37.0	33.0	39.0	26.0	40.0
33	35.007667681888506	37.0	34.0	39.0	27.0	40.0
34	35.34291225953352	38.0	34.0	40.0	28.0	40.0
35	35.490819215911415	38.0	34.0	40.0	29.0	41.0
36	35.52592464653738	38.0	35.0	40.0	29.0	41.0
37	35.80549338808395	38.0	35.0	40.0	30.0	41.0
38	35.25524720489642	38.0	34.0	40.0	28.0	41.0
39	35.307792233066394	38.0	34.0	40.0	29.0	41.0
40	34.8223613151826	37.0	33.0	40.0	26.0	41.0
41	34.28311309830786	37.0	33.0	39.0	25.0	40.0
42	35.26692387782308	38.0	34.0	40.0	29.0	40.0
43	35.421321118236044	38.0	35.0	40.0	30.0	40.0
44	36.02697797974097	38.0	35.0	40.0	30.0	41.0
45	36.17313830046025	38.0	35.0	40.0	31.0	41.0
46	35.809675096575816	38.0	35.0	40.0	30.0	41.0
47	35.26689225350057	38.0	34.0	40.0	26.0	41.0
48	35.50618863665113	38.0	34.0	40.0	28.0	41.0
49	35.90534353744806	38.0	35.0	40.0	30.0	41.0
50	35.461211552121746	38.0	34.0	40.0	28.0	41.0
51	35.09912765522677	37.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	8.0
12	11.0
13	8.0
14	18.0
15	31.0
16	79.0
17	219.0
18	608.0
19	1135.0
20	1712.0
21	2219.0
22	2486.0
23	2688.0
24	2957.0
25	3753.0
26	5253.0
27	6837.0
28	9038.0
29	12176.0
30	16347.0
31	22166.0
32	30459.0
33	39360.0
34	47203.0
35	55774.0
36	58742.0
37	54310.0
38	32105.0
39	3368.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.393795794451634	3.163405307047845	37.77355038970896	13.669248508791561
2	14.791182165828218	32.05173739162588	36.7321371230624	16.4249433194835
3	14.703850382897565	32.26386361646021	38.092956047056994	14.939329953585226
4	15.112777199349999	3.2261674240286466	62.82171666553143	18.83933871108992
5	36.06802635035857	3.841382128852086	38.494098414891646	21.596493105897693
6	15.921873327559869	27.056797283227436	40.86203037881073	16.15929901040197
7	58.40623145111853	1.5989743988946081	34.932713172260115	5.062080977726746
8	54.55317264933978	22.63960922067939	13.427200809582656	9.380017320398174
9	51.28710992614505	3.8447878251223617	13.747822787027216	31.12027946170538
10	40.067286827739885	22.05893800659732	20.73971722990396	17.134057935758836
11	34.170080471737585	19.92964804561687	27.07650166879117	18.823769813854373
12	24.39159668771711	15.088450797419457	30.264719905808175	30.25523260905526
13	24.213770689604843	19.83842403837733	40.25751929083673	15.690285981181095
14	20.69398359427454	29.623232686899748	24.524418842257877	25.158364876567834
15	12.391382615380124	22.934931740116184	43.30221175646353	21.371473888040168
16	14.579299205013186	23.871741478461402	25.275861397892363	36.27309791863305
17	14.28981502203972	28.19794879778922	35.153353637770145	22.358882542400917
18	14.382255349375782	23.769084062314512	32.579377049499364	29.269283538810342
19	14.626249160739132	28.43318510445757	28.26387334702099	28.67669238778231
20	19.49298913096362	24.763304109215813	41.321799375298	14.42190738452257
21	21.26760015179675	34.26543996730532	26.504344695384795	17.962615185513144
22	15.431453064640117	21.19340462590859	35.18327511214471	28.191867197306582
23	18.64983604005099	32.24683513510884	27.739396121398475	21.363932703441698
24	22.039476885052885	21.946306765658903	25.144498827467427	30.86971752182078
25	13.946082962761144	36.655508956981194	22.03874709299497	27.359660987262696
26	15.568653971528379	21.861650886940616	31.319269429497222	31.250425712033785
27	20.98857632165342	27.845459233815646	25.770173885121	25.395790559409935
28	11.87663595052983	29.142299720732908	34.86557230293182	24.115492025805445
29	19.992653426616975	18.747871439831076	29.389942492385835	31.86953264116611
30	15.610981910887526	32.08725393844447	33.323278420535374	18.978485730132626
31	21.592114353550194	25.393844447255496	22.21341065885627	30.80063054033804
32	24.124249530500443	31.082816802732342	25.650731251642036	19.142202415125183
33	16.642664616761866	25.500394087711275	21.869192071539086	35.987749223987784
34	23.88220183129154	22.301958761883448	22.757105742003912	31.058733664821105
35	21.039175237668946	20.155640319551615	28.852572273740133	29.9526121690393
36	15.659391450729304	29.139623816520544	20.430042133328143	34.77094259942201
37	15.974175091710535	20.162694976111474	43.01759285387617	20.845537078301824
38	19.32027167725676	34.83419124444141	20.712714923761055	25.13282215454077
39	19.189152370851133	22.18616508869406	32.000408683552436	26.624273856902374
40	22.399021105586314	26.82302056067491	27.24265099397678	23.535307339761992
41	17.35713104146192	24.16609094182098	20.914624059784565	37.562153956932534
42	25.133065418560076	17.90934036528525	31.994570347089102	24.963023869065573
43	21.54540766184355	19.71022390020337	22.016610067238172	36.7277583707149
44	19.965407856454767	18.0861933073203	33.73682725335461	28.21157158287032
45	18.48782220319357	18.91766972530627	27.467426947815003	35.12708112368516
46	27.95006276211698	23.05923965398126	27.094016678181166	21.896680905720597
47	13.424768169389603	21.11458708365363	43.41484299740194	22.045801749554826
48	20.190183810292986	23.459652229758003	25.527639657873486	30.82252430207553
49	20.489155290019365	15.818242855335754	39.37617374889315	24.316428105751733
50	23.54820033278518	16.009448374509823	29.520818534772157	30.92153275793284
51	18.89893839581975	16.41399643861476	26.900378518814037	37.786686646751455
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	69.0
1	202.0
2	335.0
3	1268.5
4	2202.0
5	1534.0
6	866.0
7	928.0
8	990.0
9	1109.0
10	1228.0
11	1282.5
12	1337.0
13	1284.0
14	1231.0
15	1126.5
16	1022.0
17	993.0
18	964.0
19	970.5
20	977.0
21	1120.5
22	1264.0
23	1375.5
24	1487.0
25	1575.0
26	2163.5
27	2664.0
28	3170.0
29	3676.0
30	3987.0
31	4298.0
32	4727.5
33	5157.0
34	6247.0
35	7337.0
36	7706.5
37	8076.0
38	8705.0
39	9334.0
40	10526.0
41	11718.0
42	13249.5
43	14781.0
44	17733.0
45	20685.0
46	68247.5
47	115810.0
48	85485.5
49	55161.0
50	49405.0
51	43649.0
52	33540.0
53	23431.0
54	19998.0
55	16565.0
56	14962.0
57	13359.0
58	12934.0
59	12509.0
60	10941.0
61	9373.0
62	8198.5
63	7024.0
64	5564.0
65	4104.0
66	3507.5
67	2911.0
68	2205.0
69	1499.0
70	1224.5
71	950.0
72	787.0
73	624.0
74	499.5
75	284.5
76	194.0
77	146.0
78	98.0
79	80.5
80	63.0
81	36.5
82	10.0
83	8.0
84	6.0
85	3.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	411076.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.8414847040522
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.31381262046052	21.43320689992814
2	10.306098637768422	5.944863724393024
3	3.714980862614144	3.214366911747975
4	1.9256798379087183	2.221578623597841
5	1.2259133629437586	1.7678580752917807
6	0.9414726177835552	1.6292080863053036
7	0.7870664766739434	1.5890116023644658
8	0.6988539563613004	1.612478855420865
9	0.6174328866243574	1.602691303981968
>10	5.286342577116342	24.947130848586525
>50	0.10007835963674609	2.024644320165282
>100	0.06445724857959917	3.690689078489039
>500	0.0033924867673473247	0.7651428577355325
>1k	0.009329338610205145	6.480720867277877
>5k	0.002544365075510494	5.075431315586497
>10k+	0.002544365075510494	16.000976629127916
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGC	26680	6.4902840350689415	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCG	21524	5.2360147515301305	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTC	17210	4.186573772246495	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTATACGCT	8336	2.027848864930086	No Hit
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	7154	1.7403107941110645	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTATACGCTCGTA	5259	1.27932547752727	No Hit
GAACTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCT	4782	1.1632885403185786	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTC	4222	1.0270606895075363	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCT	3719	0.9046988877969037	No Hit
CTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGCT	2572	0.6256750576535726	Illumina Single End Adapter 2 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	2130	0.5181523611205714	TruSeq Adapter, Index 13 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	1869	0.4546604520818535	TruSeq Adapter, Index 13 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTATACGCTCGT	1842	0.4480923235606068	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATG	1712	0.41646800105090054	No Hit
TGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGCTT	1242	0.30213391197734724	TruSeq Adapter, Index 13 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1222	0.2972686315912386	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTAT	1182	0.2875380708190213	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTT	978	0.23791221088071304	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCTATACGC	951	0.23134408235946638	No Hit
CGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTG	674	0.16395994901186153	No Hit
TCCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTG	525	0.1277136101353521	No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCCTATACGCTCG	483	0.11749652132452394	No Hit
GAATGCTACGGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGC	454	0.1104418647646664	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGC	423	0.10290068016619797	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.4326401930543259E-4	0.0	0.0	0.7023032237347838	0.0
2	2.4326401930543259E-4	0.0	0.0	3.7995407175315514	0.0
3	2.4326401930543259E-4	0.0	0.0	4.574823147057965	0.0
4	2.4326401930543259E-4	0.0	0.0	6.915266276795531	0.0
5	2.4326401930543259E-4	0.0	0.0	12.86672050910294	0.0
6	2.4326401930543259E-4	0.0	0.0	13.865076044332435	0.0
7	2.4326401930543259E-4	0.0	0.0	15.56013973085269	0.0
8	2.4326401930543259E-4	0.0	0.0	17.6787260749837	0.0
9	2.4326401930543259E-4	0.0	0.0	18.124872286389866	0.0
10	2.4326401930543259E-4	0.0	0.0	24.624886882231024	0.0
11	2.4326401930543259E-4	0.0	0.0	26.339168426276405	0.0
12	2.4326401930543259E-4	0.0	0.0	34.84148916502058	0.0
13	2.4326401930543259E-4	0.0	0.0	35.80870690577898	0.0
14	2.4326401930543259E-4	0.0	0.0	36.2616645097257	0.0
15	2.4326401930543259E-4	0.0	0.0	37.9545388200722	0.0
16	2.4326401930543259E-4	0.0	0.0	38.921026768772684	0.0
17	2.4326401930543259E-4	0.0	0.0	39.82669871264681	0.0
18	2.4326401930543259E-4	0.0	0.0	40.52219054384104	0.0
19	2.4326401930543259E-4	0.0	0.0	43.45181912833637	0.0
20	2.4326401930543259E-4	0.0	0.0	44.28694450661192	0.0
21	2.4326401930543259E-4	0.0	0.0	45.00360030748572	0.0
22	2.4326401930543259E-4	0.0	0.0	45.89418988216291	0.0
23	2.4326401930543259E-4	0.0	0.0	46.61254853117185	0.0
24	2.4326401930543259E-4	0.0	0.0	47.16743375920754	0.0
25	2.4326401930543259E-4	0.0	0.0	47.66296256653271	0.0
26	2.4326401930543259E-4	0.0	0.0	48.15265303739454	0.0
27	2.4326401930543259E-4	0.0	0.0	48.6386945479668	0.0
28	2.4326401930543259E-4	0.0	0.0	49.06562290184783	0.0
29	2.4326401930543259E-4	0.0	0.0	49.523202522161355	0.0
30	2.4326401930543259E-4	0.0	0.0	50.011919936945965	0.0
31	2.4326401930543259E-4	0.0	0.0	50.50282672790433	0.0
32	2.4326401930543259E-4	0.0	0.0	50.958946764102016	0.0
33	2.4326401930543259E-4	0.0	0.0	51.39074039836916	0.0
34	2.4326401930543259E-4	0.0	0.0	51.81499284803783	0.0
35	2.4326401930543259E-4	0.0	0.0	52.249219122498026	0.0
36	2.4326401930543259E-4	0.0	0.0	52.70387957457989	0.0
37	2.4326401930543259E-4	0.0	0.0	53.146376825696464	0.0
38	2.4326401930543259E-4	0.0	0.0	53.64117584096371	0.0
39	2.4326401930543259E-4	0.0	0.0	54.06859072288336	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGAA	20	7.0279924E-4	45.000004	31
GATCGTT	20	7.0279924E-4	45.000004	9
TAGGTTG	20	7.0279924E-4	45.000004	37
GGACTAA	65	0.0	45.000004	8
TATATGA	20	7.0279924E-4	45.000004	14
ATCGTTT	20	7.0279924E-4	45.000004	10
CTGCGAT	40	6.7975634E-9	45.000004	31
GTTCGTG	20	7.0279924E-4	45.000004	1
CGTAATG	20	7.0279924E-4	45.000004	42
GCGTCGG	20	7.0279924E-4	45.000004	2
GTTATGG	20	7.0279924E-4	45.000004	2
TGCCGTG	20	7.0279924E-4	45.000004	1
TCTGCGA	25	3.8864182E-5	45.0	30
ACTGACG	25	3.8864182E-5	45.0	43
ACCGACG	25	3.8864182E-5	45.0	17
CTCTTCG	45	3.8380676E-10	45.0	1
CGCTCGG	25	3.8864182E-5	45.0	2
TTGCGCG	55	1.8189894E-12	45.0	1
GTTTTCG	25	3.8864182E-5	45.0	1
GGACCAT	25	3.8864182E-5	45.0	8
>>END_MODULE