SRR3549576_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549576_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1110774
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGC	36106	3.2505262096520084	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCG	30829	2.775452072158693	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTC	26209	2.359525880152038	No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	15447	1.390651923793679	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	12072	1.0868097380745319	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTAGTTGT	11390	1.0254111097306922	No Hit
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT	9021	0.8121364021844226	TruSeq Adapter, Index 20 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT	7858	0.7074346356684618	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTA	7788	0.701132723668361	No Hit
TCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	6502	0.58535759749508	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCT	6243	0.5620405230947069	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTC	6115	0.5505170268659512	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG	3010	0.2709822160043357	No Hit
TGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCTT	3003	0.27035202480432563	TruSeq Adapter, Index 22 (95% over 24bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGT	2555	0.23001978800368034	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2518	0.22668877737505558	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATG	2227	0.2004908289174936	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTAT	1885	0.16970148743128666	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT	1684	0.15160599725956855	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCC	1542	0.13882211863079258	No Hit
TCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG	1524	0.13720162697362379	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCTAGTTG	1458	0.1312598242306716	No Hit
TGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG	1399	0.12594821268772946	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTC	1300	0.11703550857330115	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT	1135	0.10218100171592061	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGATCT	35	1.2118835E-7	45.000004	6
AATACCG	35	1.2118835E-7	45.000004	26
GCACGAA	25	3.8907212E-5	45.0	9
ATTCGCG	20	7.0331857E-4	45.0	14
TCTAGCG	25	3.8907212E-5	45.0	1
GCCGATC	50	2.1827873E-11	45.0	9
GCGATAG	20	7.0331857E-4	45.0	9
CGAGCAT	20	7.0331857E-4	45.0	16
CGGTAAG	20	7.0331857E-4	45.0	11
GCTCGAA	20	7.0331857E-4	45.0	13
TACCGTG	20	7.0331857E-4	45.0	17
TACGGCT	4190	0.0	43.17422	7
ACGGCTG	4235	0.0	42.66234	8
ATACGGC	8140	0.0	42.512287	6
TGATACG	8195	0.0	42.3917	4
GATACGG	8220	0.0	42.262775	5
ATGATAC	11690	0.0	42.2284	3
TGCTACG	150	0.0	42.000004	4
AATGATA	11655	0.0	41.891888	2
GAATGAT	12380	0.0	41.801292	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content