##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549576_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1110774
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.848617270479863	31.0	16.0	33.0	16.0	34.0
2	28.00900723279443	31.0	25.0	33.0	16.0	34.0
3	28.91918157969128	31.0	28.0	34.0	16.0	34.0
4	32.49962998773828	35.0	30.0	37.0	26.0	37.0
5	33.82350865252518	35.0	33.0	37.0	28.0	37.0
6	32.97697281355163	35.0	33.0	37.0	26.0	37.0
7	33.620334109368784	35.0	32.0	35.0	30.0	37.0
8	33.45289590861867	35.0	33.0	37.0	28.0	37.0
9	34.76379893659736	37.0	32.0	39.0	29.0	39.0
10	33.94781476700031	35.0	32.0	39.0	27.0	39.0
11	34.56131940430727	37.0	33.0	39.0	27.0	39.0
12	34.6398385270091	37.0	34.0	39.0	27.0	39.0
13	35.036902196126306	37.0	34.0	39.0	30.0	39.0
14	34.869915032220774	37.0	33.0	39.0	27.0	40.0
15	35.2849481532697	37.0	33.0	39.0	27.0	40.0
16	34.75827215977328	36.0	32.0	39.0	27.0	40.0
17	34.53968133931835	36.0	32.0	39.0	27.0	40.0
18	34.558226966061504	37.0	32.0	39.0	27.0	40.0
19	34.318937965778815	37.0	32.0	39.0	27.0	40.0
20	35.21916069335436	37.0	33.0	39.0	27.0	40.0
21	34.65687709651108	37.0	32.0	39.0	27.0	40.0
22	35.61606141303272	37.0	34.0	39.0	30.0	40.0
23	36.37794816947462	38.0	35.0	40.0	31.0	40.0
24	36.542717960629254	38.0	35.0	40.0	31.0	40.0
25	34.499398617540564	37.0	32.0	39.0	25.0	40.0
26	35.090713322422026	37.0	33.0	39.0	27.0	40.0
27	35.866987343960155	37.0	34.0	39.0	30.0	40.0
28	36.09706745026441	38.0	35.0	40.0	30.0	40.0
29	36.554588962291156	38.0	35.0	40.0	31.0	41.0
30	34.63867087274279	37.0	33.0	39.0	25.0	40.0
31	35.28626435260458	37.0	34.0	39.0	28.0	40.0
32	35.73799800859581	37.0	34.0	39.0	30.0	40.0
33	36.416950702843245	38.0	35.0	40.0	31.0	40.0
34	36.63955674151538	38.0	35.0	40.0	31.0	41.0
35	36.86998615379906	39.0	36.0	40.0	31.0	41.0
36	36.669096503879274	39.0	35.0	40.0	31.0	41.0
37	36.978921904905945	39.0	35.0	40.0	32.0	41.0
38	36.58349223154305	38.0	35.0	40.0	31.0	41.0
39	36.37763217360147	38.0	35.0	40.0	31.0	41.0
40	36.131513701256964	38.0	35.0	40.0	30.0	41.0
41	35.65139623361728	38.0	34.0	40.0	30.0	41.0
42	36.551246248111674	38.0	35.0	40.0	31.0	41.0
43	36.515780887921395	38.0	35.0	40.0	31.0	41.0
44	36.98949741351526	39.0	35.0	40.0	32.0	41.0
45	37.25759515436984	39.0	36.0	40.0	33.0	41.0
46	37.02024804325632	39.0	35.0	40.0	32.0	41.0
47	36.865663942440136	39.0	35.0	40.0	31.0	41.0
48	36.99083251858614	39.0	35.0	40.0	32.0	41.0
49	37.19296454544309	39.0	35.0	40.0	33.0	41.0
50	36.96858226786007	39.0	35.0	40.0	32.0	41.0
51	36.319452021743395	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	12.0
11	15.0
12	25.0
13	35.0
14	36.0
15	60.0
16	100.0
17	177.0
18	427.0
19	799.0
20	1254.0
21	1735.0
22	2367.0
23	3110.0
24	4240.0
25	6094.0
26	8304.0
27	11327.0
28	15772.0
29	22075.0
30	31359.0
31	45148.0
32	63583.0
33	86022.0
34	113155.0
35	152089.0
36	192395.0
37	206624.0
38	128406.0
39	14020.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.35124876887648	3.5523877944568385	46.50000810245828	16.596355334208397
2	17.229427408275672	17.603851008395946	45.84703999193355	19.31968159139483
3	17.204039705646693	17.436490231136126	46.69410699206139	18.665363071155788
4	17.615284477310418	3.3263292082817926	57.39259291268971	21.66579340171808
5	26.74729512934224	4.313388682126157	44.93992477317618	23.99939141535542
6	19.105236528762827	15.713907599565708	47.11084342989663	18.070012441774836
7	69.80952020843124	1.4747374353378815	23.05050352276881	5.665238833462072
8	67.53029869262335	11.579583245556702	11.941853158248213	8.948264903571744
9	63.16244348535346	4.297273792868756	13.47654878490134	19.06373393687645
10	34.964718295530865	27.687900509014433	20.813054680790152	16.53432651466455
11	26.131868408875253	21.6509388948607	31.455993748503296	20.76119894776075
12	21.307214608912346	18.396541510694345	35.050964462617955	25.24527941777535
13	22.139607156811376	20.420355535869582	39.46014220714565	17.979895100173394
14	19.23136479607913	25.785173221555418	30.17625547591139	24.807206506454058
15	14.482874104003155	24.570974833764563	39.77739846269358	21.168752599538703
16	16.433856031920087	24.897413875369786	28.637148510858196	30.031581581851935
17	16.37236737626196	26.0705598078457	34.13754733186048	23.419525484031855
18	15.772875490423793	23.349844342773597	34.90205928478701	25.975220882015606
19	17.135618946788455	25.45135194017865	31.337067666329965	26.075961446702927
20	19.101815490819916	25.69181489663964	37.788334980833184	17.41803463170726
21	22.09675415521069	28.86059630491891	29.802282012362554	19.240367527507846
22	17.82963951262813	22.74423059956391	32.934422303726954	26.49170758408101
23	18.057678699717496	28.24832054045197	29.969552762308084	23.72444799752245
24	22.142848140125714	22.00951768766644	28.005156764562365	27.84247740764548
25	16.368586229061897	29.821187748362853	26.28374448807768	27.526481534497567
26	16.42170234449132	22.491794010302726	33.70046472099635	27.386038924209604
27	18.77762713207187	25.3367471690911	28.96952935520637	26.916096343630656
28	13.792904767306402	23.69338857409338	37.5660575418582	24.947649116742017
29	18.909607174816838	22.05174049806711	32.429639152518874	26.609013174597173
30	20.059796142149526	24.15189768575786	31.982563509768863	23.80574266232375
31	20.359857180668612	23.0884050220837	32.89733105024064	23.65440674700704
32	20.811344161818695	29.670932160817593	29.475392834185893	20.04233084317782
33	23.0099012040253	19.981832487976853	28.586823242171675	28.421443065826175
34	20.813234735418725	21.039383348908057	37.64168048585941	20.505701429813804
35	17.610963166224632	20.8758037188483	32.16432865731463	29.34890445761244
36	22.266815751899127	20.384164555526148	35.60211168068392	21.74690801189081
37	16.145498544258327	24.096620914785547	36.03811396377661	23.719766577179517
38	22.044268230981277	24.216897406673183	31.296285292957883	22.442549069387653
39	22.53059578276049	25.59908676292387	33.05766969698606	18.81264775732957
40	23.88829770952507	19.748661743973123	34.78772459564232	21.57531595085949
41	21.24437554353991	21.807676449034638	33.416248489791805	23.53169951763365
42	19.701217349343793	19.341288146823747	37.27022778711061	23.68726671672185
43	27.04915671414707	18.882508953216405	29.08908562857971	24.97924870405681
44	20.25713601506697	19.30896834099466	33.19091012213106	27.242985521807316
45	19.364065057338397	17.465659080965164	32.566570697549636	30.6037051641468
46	25.16146398817401	21.93002356915088	31.832307922223603	21.076204520451505
47	16.399915734433826	18.812107593443848	42.21227720490397	22.575699467218353
48	19.363794975395535	21.666963756803813	32.504631905320075	26.46460936248058
49	19.34470918476666	17.114192445988113	40.840440989796306	22.700657379448923
50	24.364722256732694	16.400906034890987	32.464029586576565	26.770342121799757
51	19.70292786831525	16.40270658117673	30.814729188835894	33.07963636167213
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	165.0
1	542.5
2	920.0
3	2816.5
4	4713.0
5	3274.5
6	1836.0
7	1923.5
8	2011.0
9	1988.0
10	1965.0
11	2127.5
12	2290.0
13	2304.5
14	2319.0
15	2241.0
16	2163.0
17	2223.5
18	2284.0
19	2630.5
20	2977.0
21	3260.0
22	3543.0
23	4336.5
24	5130.0
25	5757.5
26	7657.0
27	8929.0
28	10538.5
29	12148.0
30	13962.5
31	15777.0
32	18130.5
33	20484.0
34	23172.0
35	25860.0
36	28129.0
37	30398.0
38	34529.5
39	38661.0
40	43425.5
41	48190.0
42	57016.5
43	65843.0
44	145313.5
45	224784.0
46	189520.0
47	154256.0
48	146619.5
49	138983.0
50	107586.0
51	76189.0
52	64113.0
53	52037.0
54	45102.5
55	38168.0
56	34171.0
57	30174.0
58	28046.0
59	25918.0
60	23201.0
61	20484.0
62	17893.0
63	15302.0
64	13368.5
65	11435.0
66	9014.0
67	6593.0
68	5276.5
69	3960.0
70	3299.5
71	2639.0
72	2356.0
73	2073.0
74	1689.0
75	1008.5
76	712.0
77	565.5
78	419.0
79	303.5
80	188.0
81	148.5
82	109.0
83	74.0
84	39.0
85	25.5
86	12.0
87	7.5
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1110774.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.6517340977647
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.39646448887997	21.27121976838119
2	12.819559520840338	7.858834593665318
3	5.143076953281223	4.729326816489535
4	2.5411790648184613	3.115661799584873
5	1.5256145665980105	2.3381366015519376
6	1.066854455156624	1.9620563448285877
7	0.8172119099377521	1.7534273515456884
8	0.6751744782550289	1.6556214861656124
9	0.5671644504962198	1.5646116533683463
>10	5.263478519743401	27.953611947808664
>50	0.10353218261553608	2.1863983350133394
>100	0.06645010184095389	3.968325857929559
>500	0.006823001527854965	1.3823944369167922
>1k	0.003856479124439763	2.2950493710307067
>5k	0.0017799134420491214	3.957869016357115
>10k+	0.0017799134420491214	12.007454619362834
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGC	36106	3.2505262096520084	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCG	30829	2.775452072158693	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTC	26209	2.359525880152038	No Hit
GCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	15447	1.390651923793679	TruSeq Adapter, Index 22 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	12072	1.0868097380745319	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCTAGTTGT	11390	1.0254111097306922	No Hit
CTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCT	9021	0.8121364021844226	TruSeq Adapter, Index 20 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT	7858	0.7074346356684618	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTA	7788	0.701132723668361	No Hit
TCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGC	6502	0.58535759749508	TruSeq Adapter, Index 22 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCT	6243	0.5620405230947069	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTC	6115	0.5505170268659512	No Hit
CGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG	3010	0.2709822160043357	No Hit
TGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTGCTT	3003	0.27035202480432563	TruSeq Adapter, Index 22 (95% over 24bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGT	2555	0.23001978800368034	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2518	0.22668877737505558	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATG	2227	0.2004908289174936	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTAT	1885	0.16970148743128666	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCT	1684	0.15160599725956855	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCC	1542	0.13882211863079258	No Hit
TCCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG	1524	0.13720162697362379	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTCTAGTTG	1458	0.1312598242306716	No Hit
TGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTCTG	1399	0.12594821268772946	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTTC	1300	0.11703550857330115	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCTAGTTGTCGTATGCCGTCTT	1135	0.10218100171592061	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.9067551095002224	0.0
2	0.0	0.0	0.0	4.1625929306951726	0.0
3	0.0	0.0	0.0	5.22707589482649	0.0
4	0.0	0.0	0.0	7.170585555657587	0.0
5	0.0	0.0	0.0	11.416633806696952	0.0
6	0.0	0.0	0.0	12.45671936865645	0.0
7	0.0	0.0	0.0	14.007619911881266	0.0
8	0.0	0.0	0.0	15.90431536928304	0.0
9	0.0	0.0	0.0	16.515870915235684	0.0
10	0.0	0.0	0.0	20.661718765473445	0.0
11	0.0	0.0	0.0	23.01665325259684	0.0
12	0.0	0.0	0.0	28.543880213256703	0.0
13	0.0	0.0	0.0	29.44388327418539	0.0
14	0.0	0.0	0.0	29.854947991220538	0.0
15	0.0	0.0	0.0	30.994873844724488	0.0
16	0.0	0.0	0.0	32.024876347483826	0.0
17	0.0	0.0	0.0	33.349538249905024	0.0
18	0.0	0.0	0.0	34.598937317582156	0.0
19	0.0	0.0	0.0	36.70548644458729	0.0
20	0.0	0.0	0.0	37.841361068948316	0.0
21	0.0	0.0	0.0	39.0946313111398	0.0
22	0.0	0.0	0.0	40.53821929573433	0.0
23	0.0	0.0	0.0	41.80841467301179	0.0
24	0.0	0.0	0.0	42.81428985554217	0.0
25	0.0	0.0	0.0	43.686474476356125	0.0
26	9.002731428715473E-5	0.0	0.0	44.45872877831134	0.0
27	9.002731428715473E-5	0.0	0.0	45.243586904266756	0.0
28	9.002731428715473E-5	0.0	0.0	46.00530801045037	0.0
29	9.002731428715473E-5	0.0	0.0	46.825276788977774	0.0
30	9.002731428715473E-5	0.0	0.0	47.679906083505735	0.0
31	9.002731428715473E-5	0.0	0.0	48.478178279289935	0.0
32	9.002731428715473E-5	0.0	0.0	49.221713867987546	0.0
33	9.002731428715473E-5	0.0	0.0	49.95435615165641	0.0
34	9.002731428715473E-5	0.0	0.0	50.72264925178299	0.0
35	9.002731428715473E-5	0.0	0.0	51.462313665966256	0.0
36	9.002731428715473E-5	0.0	0.0	52.18505294506353	0.0
37	9.002731428715473E-5	0.0	0.0	52.89464823627488	0.0
38	9.002731428715473E-5	0.0	0.0	53.61378642280068	0.0
39	9.002731428715473E-5	0.0	0.0	54.3649743332127	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGATCT	35	1.2118835E-7	45.000004	6
AATACCG	35	1.2118835E-7	45.000004	26
GCACGAA	25	3.8907212E-5	45.0	9
ATTCGCG	20	7.0331857E-4	45.0	14
TCTAGCG	25	3.8907212E-5	45.0	1
GCCGATC	50	2.1827873E-11	45.0	9
GCGATAG	20	7.0331857E-4	45.0	9
CGAGCAT	20	7.0331857E-4	45.0	16
CGGTAAG	20	7.0331857E-4	45.0	11
GCTCGAA	20	7.0331857E-4	45.0	13
TACCGTG	20	7.0331857E-4	45.0	17
TACGGCT	4190	0.0	43.17422	7
ACGGCTG	4235	0.0	42.66234	8
ATACGGC	8140	0.0	42.512287	6
TGATACG	8195	0.0	42.3917	4
GATACGG	8220	0.0	42.262775	5
ATGATAC	11690	0.0	42.2284	3
TGCTACG	150	0.0	42.000004	4
AATGATA	11655	0.0	41.891888	2
GAATGAT	12380	0.0	41.801292	1
>>END_MODULE