FastQCFastQC Report
Sat 18 Jun 2016
SRR3549575_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549575_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583284
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGC160542.7523470556367053No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCG140182.4032889638666584No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC114451.9621659431769085No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC73501.2601065690126936Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC63931.0960355504351225Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCCCAGAT54400.9326503041400073No Hit
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT40450.6934872206335164Illumina Single End Adapter 2 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT33160.5685052221559309No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTA32470.5566756502835668No Hit
TCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC27400.4697540134822831Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCT26720.4580958846805329No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTC25720.4409515776191358No Hit
TGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCTT15200.2605934673332373Illumina Single End Adapter 2 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12190.20898910307843177No Hit
CGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG12130.20796044465474797No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGT11870.20350292481878468No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATG9180.15738473882362625No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTAT7820.13406848122012605No Hit
TCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG7680.13166827823153043No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGCCCAGA7390.12669642918372526No Hit
TTCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT6470.11092366668723984No Hit
TGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG6430.11023789440478395No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGCGG502.1827873E-1145.0000042
ATCACGC253.8884336E-545.00000425
CGCTATA253.8884336E-545.00000429
GGAACCG253.8884336E-545.0000048
ACGCTAT253.8884336E-545.00000428
TTTAGCG406.8048394E-945.01
CGACGTT351.2105556E-745.027
TTTCGCA207.0304266E-445.013
GATCCAT207.0304266E-445.09
CTTTACG406.8048394E-945.01
TTCGCAA207.0304266E-445.014
CGATCTA351.2105556E-745.010
TCGACGT351.2105556E-745.026
TTTCGAC302.163657E-644.99999624
TGCTACG600.044.9999964
GATTCGA302.163657E-644.9999969
TTCGACG302.163657E-644.99999625
TACGGCT17850.042.8571437
TAGCGGG850.042.352943
CGTTCAT850.042.3529417
Produced by FastQC (version 0.11.5)