##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549575_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	583284
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.88509713964381	31.0	16.0	33.0	16.0	34.0
2	28.173418094787444	31.0	25.0	33.0	16.0	34.0
3	29.117486507430343	31.0	28.0	34.0	16.0	34.0
4	32.537174001001226	35.0	32.0	37.0	27.0	37.0
5	33.79298249223363	35.0	33.0	37.0	28.0	37.0
6	33.08612099766152	35.0	33.0	37.0	28.0	37.0
7	33.53168782274158	35.0	32.0	35.0	29.0	37.0
8	33.46898080523381	35.0	33.0	37.0	28.0	37.0
9	34.71968372182333	37.0	32.0	39.0	28.0	39.0
10	33.763375988369305	35.0	32.0	39.0	27.0	39.0
11	34.14547973200019	35.0	32.0	39.0	27.0	39.0
12	34.37684729908587	37.0	32.0	39.0	27.0	39.0
13	34.745530479149096	37.0	34.0	39.0	27.0	39.0
14	34.56022280741457	37.0	33.0	39.0	26.0	40.0
15	34.970779243044554	37.0	32.0	39.0	27.0	40.0
16	34.530796318774385	36.0	32.0	39.0	27.0	40.0
17	34.45691635635471	36.0	32.0	39.0	27.0	40.0
18	34.356939329726174	36.0	32.0	39.0	27.0	40.0
19	34.09167060985729	36.0	32.0	39.0	25.0	40.0
20	35.068409214036386	37.0	33.0	39.0	27.0	40.0
21	34.55932616015526	37.0	32.0	39.0	26.0	40.0
22	35.544943800961455	37.0	34.0	39.0	29.0	40.0
23	36.290124536246495	38.0	34.0	40.0	30.0	40.0
24	36.41627577646567	38.0	35.0	40.0	31.0	41.0
25	34.391999437666726	37.0	32.0	39.0	25.0	40.0
26	34.96760583180749	37.0	33.0	39.0	27.0	40.0
27	35.551360915094534	37.0	34.0	39.0	30.0	40.0
28	35.840619663834424	38.0	34.0	40.0	30.0	40.0
29	36.372658944870764	38.0	35.0	40.0	30.0	41.0
30	34.47177704171553	37.0	33.0	39.0	25.0	40.0
31	34.787600894247056	37.0	33.0	39.0	26.0	40.0
32	35.327209386851	37.0	34.0	39.0	30.0	40.0
33	36.10747080324507	38.0	35.0	40.0	30.0	40.0
34	36.37767708354764	38.0	35.0	40.0	30.0	41.0
35	36.705385369734124	38.0	35.0	40.0	31.0	41.0
36	36.56884125057434	38.0	35.0	40.0	31.0	41.0
37	36.79269789673641	39.0	35.0	40.0	31.0	41.0
38	36.288662126854156	38.0	35.0	40.0	30.0	41.0
39	36.22187647869649	38.0	35.0	40.0	30.0	41.0
40	35.884250210874974	38.0	34.0	40.0	30.0	41.0
41	35.33251212102509	37.0	33.0	40.0	29.0	41.0
42	36.26997826101864	38.0	35.0	40.0	31.0	41.0
43	36.30071286028761	38.0	35.0	40.0	31.0	41.0
44	36.730155464576434	39.0	35.0	40.0	32.0	41.0
45	36.988775622166905	39.0	35.0	40.0	33.0	41.0
46	36.78314337441109	39.0	35.0	40.0	32.0	41.0
47	36.6155766316237	39.0	35.0	40.0	31.0	41.0
48	36.67030297419439	38.0	35.0	40.0	32.0	41.0
49	36.89756447973886	39.0	35.0	40.0	32.0	41.0
50	36.731427572160385	39.0	35.0	40.0	32.0	41.0
51	36.04955219069956	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	5.0
10	12.0
11	7.0
12	17.0
13	17.0
14	27.0
15	35.0
16	61.0
17	114.0
18	267.0
19	521.0
20	796.0
21	1100.0
22	1501.0
23	1868.0
24	2595.0
25	3525.0
26	4798.0
27	6620.0
28	9076.0
29	12646.0
30	17827.0
31	25381.0
32	36440.0
33	47848.0
34	60853.0
35	79936.0
36	98509.0
37	103186.0
38	61298.0
39	6396.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.68819305861296	3.775862187202118	45.76038430678709	17.775560447397837
2	17.917858196007433	15.68618374582536	45.90851111979756	20.487446938369644
3	18.057755741628434	15.771390951920505	47.468300176243474	18.702553130207583
4	17.884769683378938	4.073830243929201	55.0395690606977	23.001831011994156
5	25.227333511634125	4.82612243778331	44.73326887073878	25.21327517984378
6	19.38352500668628	14.451450751263536	47.91559514747533	18.249429094574857
7	71.51301938678243	1.7550627138752308	21.26082662990927	5.471091269433072
8	69.45278800721432	10.76851070833419	11.199518587857717	8.579182696593769
9	64.81953902387173	4.600160470714094	13.12653870155876	17.45376180385541
10	36.05464919318891	26.488811625211735	20.74444010121999	16.71209908037937
11	29.79149093751929	23.226078548357233	27.461236721734185	19.521193792389298
12	25.13938321640916	18.255086715905115	32.570925998313	24.034604069372726
13	23.236879461805913	20.987374932279987	36.27992538797567	19.49582021793843
14	20.00294882081456	25.915334554007995	28.575273794583772	25.506442830593674
15	14.915375700344944	24.310798856131832	38.97055293819135	21.80327250533188
16	16.920230968104732	23.43352466380014	29.71588454337852	29.930359824716607
17	16.696840647094728	25.359516119077497	32.9513581720054	24.992285061822372
18	16.941489908860863	23.759095054896072	32.959758882465486	26.339656153777575
19	18.951145582597842	25.991112391219374	29.02548329801606	26.032258728166724
20	21.722865705213927	24.679058571810643	35.84497431782802	17.753101405147405
21	23.64028500696059	27.600105608931496	28.44960602382373	20.310003360284185
22	18.561112596951055	23.920937313555658	30.3941133307274	27.123836758765883
23	19.46410324987485	28.300964881601416	28.353426461209292	23.881505407314446
24	23.121841161423937	21.88059333017878	27.2455613389018	27.752004169495475
25	18.39498426152612	28.80003566015869	25.26607964559289	27.538900432722308
26	19.00446437755879	23.70560481686451	31.00547932053682	26.284451485039877
27	23.269796531363795	26.17712812283553	26.126895303145638	24.426180042655034
28	15.495710494373238	23.795612428936845	33.13120195307946	27.577475123610455
29	20.90113906776116	20.13513142825793	30.007166320351665	28.956563183629243
30	21.87750735490773	24.23039205601388	27.637994527537185	26.254106061541204
31	20.91742615946949	26.01734318102331	23.443639804966363	29.62159085454084
32	23.398721720465502	27.73005945645689	23.39683584668875	25.47438297638886
33	19.94757270900625	25.570905425144524	22.761467826993368	31.720054038855857
34	21.653259818544655	25.193044897511328	28.755117575657827	24.398577708286187
35	23.895735182175407	21.63028644708238	25.308426084034537	29.165552286707673
36	19.905226270564597	26.514356642733215	27.31482433942985	26.265592747272343
37	22.125585478086148	23.950082635560037	28.6455654535355	25.278766432818315
38	20.401039630780204	23.98437124968283	23.117897970799817	32.49669114873715
39	19.967631548268084	25.877274192331694	26.670712723133157	27.484381536267065
40	23.42752415632865	21.872192619718696	28.08048909279185	26.619794131160806
41	19.465303351369144	30.764944692465416	24.75877959964614	25.010972356519297
42	23.660858175434267	22.24010944925628	28.798664115593773	25.30036825971568
43	22.900336714190686	24.714547287427738	25.68628661166773	26.698829386713847
44	21.98294484333532	20.65083218466476	29.26413205231071	28.10209091968921
45	20.397953655509152	19.10252981394998	26.987882403769003	33.51163412677187
46	24.244107501663	23.24973769210196	27.65702470837534	24.849130097859703
47	16.572544420899597	21.364892573771954	36.58543693980977	25.477126065518686
48	20.054381741998753	23.799898505702195	27.19567140535314	28.950048346945913
49	20.21279513924606	19.37563862543804	34.304386885290874	26.107179350025028
50	22.31605872953827	19.513993183423512	28.688083335047764	29.481864751990454
51	19.098586623325858	19.849507272615057	26.219474561277185	34.832431542781904
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	132.0
1	348.5
2	565.0
3	1471.5
4	2378.0
5	1697.5
6	1017.0
7	991.0
8	965.0
9	1079.5
10	1194.0
11	1108.5
12	1023.0
13	1156.0
14	1289.0
15	1189.0
16	1089.0
17	1193.0
18	1297.0
19	1365.5
20	1434.0
21	1438.0
22	1442.0
23	1762.5
24	2083.0
25	2511.0
26	3355.0
27	3771.0
28	4554.5
29	5338.0
30	6626.5
31	7915.0
32	8987.5
33	10060.0
34	11422.5
35	12785.0
36	13501.5
37	14218.0
38	15551.0
39	16884.0
40	18216.5
41	19549.0
42	21813.5
43	24078.0
44	25971.0
45	27864.0
46	30939.5
47	34015.0
48	68724.0
49	103433.0
50	89389.0
51	75345.0
52	72313.0
53	69281.0
54	53926.5
55	38572.0
56	32851.5
57	27131.0
58	24221.0
59	21311.0
60	18940.5
61	16570.0
62	14628.5
63	12687.0
64	10391.5
65	8096.0
66	6646.0
67	5196.0
68	4444.0
69	3692.0
70	3167.5
71	2643.0
72	2253.0
73	1863.0
74	1481.0
75	811.0
76	523.0
77	396.0
78	269.0
79	196.5
80	124.0
81	84.5
82	45.0
83	49.0
84	53.0
85	38.0
86	23.0
87	12.5
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	583284.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.585582294735165
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.22328442255163	21.47820044712547
2	12.377065979734715	7.571195401810861
3	4.902175644124599	4.498076897598579
4	2.4163446923829985	2.956212377653069
5	1.4798426261694801	2.263092421298182
6	0.9815047711573617	1.8011936970545213
7	0.7132207691478739	1.5269990770360626
8	0.6009374317715229	1.470401701874777
9	0.503030125174445	1.3846922361228526
>10	5.601247533512663	33.23278074654615
>50	0.12194194624966531	2.524202869950331
>100	0.06635910563353851	3.7771969823584857
>500	0.003970202901151877	0.8692722549186356
>1k	0.005671718430216966	4.116683273094021
>5k	0.00170151552906509	3.3277289295757706
>10k+	0.00170151552906509	7.202070685982237
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGC	16054	2.7523470556367053	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCG	14018	2.4032889638666584	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTC	11445	1.9621659431769085	No Hit
GCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	7350	1.2601065690126936	Illumina Single End Adapter 2 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	6393	1.0960355504351225	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCCCAGAT	5440	0.9326503041400073	No Hit
CTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCT	4045	0.6934872206335164	Illumina Single End Adapter 2 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT	3316	0.5685052221559309	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTA	3247	0.5566756502835668	No Hit
TCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGC	2740	0.4697540134822831	Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCT	2672	0.4580958846805329	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTC	2572	0.4409515776191358	No Hit
TGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTGCTT	1520	0.2605934673332373	Illumina Single End Adapter 2 (95% over 23bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1219	0.20898910307843177	No Hit
CGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG	1213	0.20796044465474797	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGCCCAGATCGT	1187	0.20350292481878468	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATG	918	0.15738473882362625	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTAT	782	0.13406848122012605	No Hit
TCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG	768	0.13166827823153043	No Hit
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGCCCAGA	739	0.12669642918372526	No Hit
TTCCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCT	647	0.11092366668723984	No Hit
TGCTGTCTCTTATACACATCTGACGCTGCCCAGATCGTATGCCGTCTTCTG	643	0.11023789440478395	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8073254195211937	0.0
2	0.0	0.0	0.0	3.8614122794384897	0.0
3	0.0	0.0	0.0	4.748801612936409	0.0
4	0.0	0.0	0.0	6.419342892998951	0.0
5	0.0	0.0	0.0	10.06405113118138	0.0
6	0.0	0.0	0.0	10.95966973206877	0.0
7	0.0	0.0	0.0	12.270009120771357	0.0
8	0.0	0.0	0.0	13.889288922720322	0.0
9	0.0	0.0	0.0	14.428648822871876	0.0
10	0.0	0.0	0.0	18.051240904945104	0.0
11	0.0	0.0	0.0	20.10958641073645	0.0
12	0.0	0.0	0.0	24.8895906625246	0.0
13	0.0	0.0	0.0	25.729318822391836	0.0
14	0.0	0.0	0.0	26.09586410736451	0.0
15	0.0	0.0	0.0	27.06828920388696	0.0
16	0.0	0.0	0.0	27.98859560694276	0.0
17	0.0	0.0	0.0	29.09800371688577	0.0
18	0.0	0.0	0.0	30.17535197262397	0.0
19	0.0	0.0	0.0	31.989733988931636	0.0
20	0.0	0.0	0.0	32.97364577118522	0.0
21	0.0	0.0	0.0	34.06813833398482	0.0
22	0.0	0.0	0.0	35.372991544427755	0.0
23	0.0	0.0	0.0	36.51154497637514	0.0
24	0.0	0.0	0.0	37.37801825525816	0.0
25	0.0	0.0	0.0	38.150026402232875	0.0
26	0.0	0.0	0.0	38.870773071094014	0.0
27	0.0	0.0	0.0	39.55448803670253	0.0
28	0.0	0.0	0.0	40.246946598912366	0.0
29	0.0	0.0	0.0	40.978665624292795	0.0
30	0.0	0.0	0.0	41.761646127786804	0.0
31	0.0	0.0	0.0	42.512566777076	0.0
32	0.0	0.0	0.0	43.19371009662532	0.0
33	0.0	0.0	0.0	43.875710631527696	0.0
34	0.0	0.0	0.0	44.50456381453974	0.0
35	0.0	0.0	0.0	45.18313548802985	0.0
36	0.0	0.0	0.0	45.89993896626686	0.0
37	0.0	0.0	0.0	46.58365393187538	0.0
38	0.0	0.0	0.0	47.28159867234486	0.0
39	0.0	0.0	0.0	47.991372984686706	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGCGG	50	2.1827873E-11	45.000004	2
ATCACGC	25	3.8884336E-5	45.000004	25
CGCTATA	25	3.8884336E-5	45.000004	29
GGAACCG	25	3.8884336E-5	45.000004	8
ACGCTAT	25	3.8884336E-5	45.000004	28
TTTAGCG	40	6.8048394E-9	45.0	1
CGACGTT	35	1.2105556E-7	45.0	27
TTTCGCA	20	7.0304266E-4	45.0	13
GATCCAT	20	7.0304266E-4	45.0	9
CTTTACG	40	6.8048394E-9	45.0	1
TTCGCAA	20	7.0304266E-4	45.0	14
CGATCTA	35	1.2105556E-7	45.0	10
TCGACGT	35	1.2105556E-7	45.0	26
TTTCGAC	30	2.163657E-6	44.999996	24
TGCTACG	60	0.0	44.999996	4
GATTCGA	30	2.163657E-6	44.999996	9
TTCGACG	30	2.163657E-6	44.999996	25
TACGGCT	1785	0.0	42.857143	7
TAGCGGG	85	0.0	42.35294	3
CGTTCAT	85	0.0	42.35294	17
>>END_MODULE