SRR3549574_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549574_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	459884
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGC	9289	2.019857181376173	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCG	8395	1.8254603334753983	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC	6452	1.4029624861921701	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	5948	1.293369632342069	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	5113	1.1118021066181907	No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	3464	0.7532334240808551	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTTGTGGT	3318	0.7214862878465005	No Hit
TCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	2607	0.5668820833079646	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTA	2044	0.4444599072809665	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT	1832	0.39836132589957474	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTC	1558	0.33878108392551165	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCT	1540	0.3348670534308652	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	1179	0.2563689973993442	No Hit
TGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCTT	987	0.2146193387897818	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	938	0.20396447799879971	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGT	707	0.15373441998416992	No Hit
TCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	654	0.14220977463882198	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATG	583	0.12677109879882753	No Hit
TTCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT	565	0.12285706830418106	No Hit
TGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	537	0.11676857642361987	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC	533	0.11589879186925398	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCC	491	0.1067660540484122	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTAT	469	0.10198223899939986	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAATTG	35	1.2098099E-7	45.000004	9
CGGGTGC	25	3.887144E-5	45.0	6
TGCCCCG	20	7.028867E-4	45.0	1
ATGATAG	20	7.028867E-4	45.0	11
GTCTGCG	40	6.7993824E-9	45.0	1
TGGGTAG	90	0.0	45.0	6
GCTATAG	25	3.887144E-5	45.0	14
ATTCGCC	20	7.028867E-4	45.0	40
TAACGAC	20	7.028867E-4	45.0	36
GCCGCGT	20	7.028867E-4	45.0	17
CTAAAGT	20	7.028867E-4	45.0	19
CATAACG	20	7.028867E-4	45.0	34
TCGACGG	20	7.028867E-4	45.0	2
ATCATAC	25	3.887144E-5	45.0	10
TACCGGG	45	3.8380676E-10	45.0	3
CTTACCG	20	7.028867E-4	45.0	1
CGAAGCA	20	7.028867E-4	45.0	29
TCGTGTA	30	2.162651E-6	44.999996	17
TTACCGG	30	2.162651E-6	44.999996	2
TACGGCT	1070	0.0	42.897194	7

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content