##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549574_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	459884
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.498427864417984	31.0	25.0	33.0	16.0	34.0
2	28.6895456245488	31.0	28.0	33.0	16.0	34.0
3	29.62442268050204	31.0	30.0	34.0	16.0	34.0
4	32.88620608675231	35.0	32.0	37.0	28.0	37.0
5	33.93742769915892	35.0	33.0	37.0	28.0	37.0
6	33.33328187108053	35.0	33.0	37.0	28.0	37.0
7	33.66402832018509	35.0	32.0	36.0	30.0	37.0
8	33.71746353428256	35.0	35.0	37.0	28.0	37.0
9	34.86375912186551	37.0	33.0	39.0	29.0	39.0
10	33.83082038079168	35.0	32.0	39.0	27.0	39.0
11	34.16683337537292	37.0	32.0	39.0	27.0	39.0
12	34.17791443059554	37.0	32.0	39.0	26.0	39.0
13	34.63275304207148	37.0	33.0	39.0	27.0	39.0
14	34.588385331953276	37.0	33.0	39.0	25.0	40.0
15	35.0038292265006	37.0	32.0	39.0	27.0	40.0
16	34.57456010646163	36.0	32.0	39.0	27.0	40.0
17	34.44884144697358	36.0	32.0	39.0	27.0	40.0
18	34.478270607370554	37.0	32.0	39.0	27.0	40.0
19	34.17344808690887	36.0	32.0	39.0	25.0	40.0
20	35.25026093536631	37.0	33.0	39.0	29.0	40.0
21	34.52539988344887	37.0	32.0	39.0	25.0	40.0
22	35.442389820041576	37.0	34.0	39.0	28.0	40.0
23	36.30532482104183	38.0	34.0	40.0	31.0	40.0
24	36.44433813744335	38.0	35.0	40.0	31.0	41.0
25	34.37296361691209	37.0	32.0	39.0	25.0	40.0
26	34.89283384505658	37.0	33.0	39.0	27.0	40.0
27	35.619982430352	37.0	34.0	39.0	30.0	40.0
28	35.79240199702534	38.0	34.0	39.0	30.0	40.0
29	36.277298188238774	38.0	35.0	40.0	30.0	41.0
30	34.27160979725322	37.0	33.0	39.0	24.0	40.0
31	34.97401736089971	37.0	33.0	39.0	27.0	40.0
32	35.354208887458576	37.0	34.0	39.0	30.0	40.0
33	36.21142288055249	38.0	35.0	40.0	30.0	40.0
34	36.406354645954195	38.0	35.0	40.0	31.0	41.0
35	36.66534604378496	38.0	35.0	40.0	31.0	41.0
36	36.3809286689687	38.0	35.0	40.0	30.0	41.0
37	36.678166668116305	38.0	35.0	40.0	31.0	41.0
38	36.25719094380322	38.0	35.0	40.0	30.0	41.0
39	36.139045933322315	38.0	35.0	40.0	30.0	41.0
40	35.81970670864827	38.0	34.0	40.0	30.0	41.0
41	35.40352567169112	37.0	34.0	40.0	29.0	41.0
42	36.192918214158354	38.0	35.0	40.0	31.0	41.0
43	36.16684859660262	38.0	35.0	40.0	31.0	41.0
44	36.54068417253046	38.0	35.0	40.0	31.0	41.0
45	36.81232658670447	39.0	35.0	40.0	32.0	41.0
46	36.63274216976455	38.0	35.0	40.0	32.0	41.0
47	36.51523862539249	38.0	35.0	40.0	31.0	41.0
48	36.62148715763105	38.0	35.0	40.0	32.0	41.0
49	36.76132242043646	39.0	35.0	40.0	32.0	41.0
50	36.591553522192555	38.0	35.0	40.0	32.0	41.0
51	35.785243670142904	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	9.0
11	13.0
12	13.0
13	18.0
14	19.0
15	28.0
16	51.0
17	116.0
18	226.0
19	344.0
20	605.0
21	829.0
22	1124.0
23	1515.0
24	2125.0
25	2911.0
26	4149.0
27	5490.0
28	7219.0
29	10049.0
30	14247.0
31	20204.0
32	28323.0
33	37091.0
34	45424.0
35	59776.0
36	77004.0
37	84392.0
38	51276.0
39	5288.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.422167329152572	3.906419879795774	48.402205773629866	19.269207017421785
2	17.937131972410434	12.943481399657305	48.518322011637714	20.601064616294543
3	18.512059562846282	12.29657913734768	49.04062763653443	20.15073366327161
4	18.616433709370188	4.218237642535944	54.32456880430717	22.840759843786692
5	23.54398065599151	5.083238381852815	46.539779596593924	24.83300136556175
6	19.880230666863817	12.355072148628784	48.706195475380746	19.05850170912665
7	72.96861817327847	1.9407067869288777	19.505353523932122	5.585321515860521
8	71.74222195162258	8.772647015334302	10.987335936888433	8.497795096154682
9	66.41392177157718	4.63899592071044	13.499056283758511	15.448026023953867
10	36.97323672926216	26.036783188804137	20.538005236103018	16.451974845830687
11	29.789033756338558	23.491358690452373	26.649328961216305	20.070278591992764
12	25.125248975828683	18.392681632759565	32.09331048699237	24.388758904419376
13	23.713153751815675	21.60240408450827	34.98730114550626	19.697141018169802
14	20.336215219490132	24.54901670856129	29.57550164824173	25.539266423706845
15	17.20781762357464	23.36306546868341	37.64818954345009	21.780927364291866
16	19.344878273651616	24.09977298623131	28.881196127719168	27.674152612397908
17	19.064155308730026	25.797809882492107	31.153943168277216	23.984091640500647
18	18.744292038861975	23.977568256342906	32.962660149081074	24.315479555714052
19	19.791512642318498	25.380095850257888	30.01278583294918	24.815605674474433
20	22.13666924702751	25.55622722251698	34.583503666141894	17.72359986431361
21	23.96930530307643	27.32384688312705	28.55241756616886	20.154430247627666
22	20.06245053100347	23.567247392820796	30.587930869523618	25.782371206652112
23	20.140296248619215	25.773890807247046	29.082333805916278	25.003479138217465
24	23.977568256342906	22.08665663515147	27.54999086726218	26.385784241243442
25	19.09698967565734	27.56651677379513	25.701481243096087	27.635012307451447
26	18.732767393516625	25.8284697880335	29.801210740099677	25.637552078350197
27	22.701811761226747	25.277243826704126	27.938349670786543	24.082594741282584
28	16.2986753181237	25.354219759765506	34.86248706195475	23.48461786015604
29	21.42127145106157	23.255212183942035	31.358125092414607	23.965391272581783
30	25.232015029877097	23.649876925485557	30.503344321611536	20.614763723025806
31	23.204329787511632	23.224987170677824	31.252881161336337	22.317801880474207
32	26.51364257073523	26.185298901462104	27.79353054248463	19.50752798531804
33	26.870036791886648	20.794809125779544	27.512372685285857	24.82278139704795
34	22.533508449956948	24.2504631602752	32.20638247905994	21.009645910707917
35	25.29420462551426	22.464143131746265	27.457576258360806	24.78407598437867
36	26.301415139469952	25.597759434987953	28.528933383200982	19.571892042341112
37	23.13018065425194	22.46653503927077	35.156039349053245	19.247244957424044
38	24.446817023423296	23.929512659714188	30.04844699967818	21.57522331718433
39	23.82339894408155	21.48911464630211	34.88096998373503	19.80651642588131
40	27.38908072470449	21.30450287463795	29.602247523288483	21.704168877369074
41	23.903201676944622	22.76182689547799	29.016012733645873	24.318958693931513
42	25.21614146175992	20.415148167798836	30.061058875716483	24.30765149472476
43	26.95179653999704	20.814596724391368	27.909864226631065	24.323742508980526
44	22.865983595863305	19.666698558766992	31.599055413973957	25.868262431395745
45	21.597837715597844	19.541232136799717	30.181958928773344	28.678971218829098
46	25.95763279435684	21.90726357081351	31.125457724121734	21.009645910707917
47	19.75302467578781	20.503213853928383	37.85498082125058	21.888780649033237
48	21.774838872411305	21.21252315801376	31.97197554165833	25.040662427916605
49	22.910125161997374	17.70033312748432	36.59422810969723	22.795313600821075
50	25.539701315984033	17.166937749519445	31.4451035478512	25.84825738664533
51	21.62480103678319	17.79927112054344	29.36697080133251	31.208957041340863
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	120.0
1	346.0
2	572.0
3	1302.0
4	2032.0
5	1438.5
6	845.0
7	899.0
8	953.0
9	982.0
10	1011.0
11	980.5
12	950.0
13	971.0
14	992.0
15	957.5
16	923.0
17	1002.0
18	1081.0
19	1096.0
20	1111.0
21	1062.5
22	1014.0
23	1269.0
24	1524.0
25	1911.0
26	2629.5
27	2961.0
28	3674.5
29	4388.0
30	4809.0
31	5230.0
32	5992.5
33	6755.0
34	8036.5
35	9318.0
36	9891.5
37	10465.0
38	12157.0
39	13849.0
40	15569.5
41	17290.0
42	20002.5
43	22715.0
44	25727.5
45	28740.0
46	50417.0
47	72094.0
48	67179.5
49	62265.0
50	60255.5
51	58246.0
52	46011.5
53	33777.0
54	28840.5
55	23904.0
56	21125.0
57	18346.0
58	16464.5
59	14583.0
60	13278.0
61	11973.0
62	10705.5
63	9438.0
64	7951.0
65	6464.0
66	5417.0
67	4370.0
68	3533.5
69	2697.0
70	2150.5
71	1604.0
72	1446.0
73	1288.0
74	1062.5
75	633.0
76	429.0
77	366.0
78	303.0
79	197.0
80	91.0
81	57.0
82	23.0
83	18.0
84	13.0
85	6.5
86	0.0
87	1.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	459884.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.720824966677604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.99040764707223	22.901438779662637
2	12.698120798748711	8.3098597612317
3	4.9952997775470305	4.903509890315998
4	2.4943043328110686	3.2646278195014617
5	1.424313928256769	2.330236337204537
6	0.9845063703270087	1.932831637322948
7	0.6737839007555894	1.543273555739224
8	0.5853069599990396	1.5321381271925396
9	0.4699600815498102	1.3839733412745185
>10	5.519354485368768	33.60797350214929
>50	0.09542499548607228	2.145649907068256
>100	0.05510457485815441	3.337653201413103
>500	0.005376056083722382	1.2102538520704735
>1k	0.005376056083722382	3.857244380999318
>5k	0.0033600350523264886	7.7393359068540075
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGC	9289	2.019857181376173	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCG	8395	1.8254603334753983	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC	6452	1.4029624861921701	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	5948	1.293369632342069	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	5113	1.1118021066181907	No Hit
CTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCT	3464	0.7532334240808551	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCAGTTGTGGT	3318	0.7214862878465005	No Hit
TCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGC	2607	0.5668820833079646	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTA	2044	0.4444599072809665	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT	1832	0.39836132589957474	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTC	1558	0.33878108392551165	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCT	1540	0.3348670534308652	No Hit
CGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	1179	0.2563689973993442	No Hit
TGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTGCTT	987	0.2146193387897818	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	938	0.20396447799879971	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGT	707	0.15373441998416992	No Hit
TCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	654	0.14220977463882198	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATG	583	0.12677109879882753	No Hit
TTCCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCT	565	0.12285706830418106	No Hit
TGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTCTG	537	0.11676857642361987	No Hit
CGTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCCGTCTTC	533	0.11589879186925398	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTATGCC	491	0.1067660540484122	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCAGTTGTGGTCGTAT	469	0.10198223899939986	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8641309547625053	0.0
2	0.0	0.0	0.0	4.067547468492054	0.0
3	0.0	0.0	0.0	5.047794661262405	0.0
4	0.0	0.0	0.0	6.610362613180715	0.0
5	0.0	0.0	0.0	9.767897991667464	0.0
6	0.0	0.0	0.0	10.761191952753302	0.0
7	0.0	0.0	0.0	12.020639987475102	0.0
8	0.0	0.0	0.0	13.68323316314549	0.0
9	0.0	0.0	0.0	14.23054509398022	0.0
10	0.0	0.0	0.0	17.316758139008968	0.0
11	0.0	0.0	0.0	19.52166198432648	0.0
12	0.0	0.0	0.0	23.60725748232163	0.0
13	0.0	0.0	0.0	24.419418809960774	0.0
14	0.0	0.0	0.0	24.761461585965158	0.0
15	0.0	0.0	0.0	25.628636786667943	0.0
16	0.0	0.0	0.0	26.57452748954084	0.0
17	0.0	0.0	0.0	27.72590479338268	0.0
18	0.0	0.0	0.0	28.914900279200843	0.0
19	0.0	0.0	0.0	30.61358951387741	0.0
20	0.0	0.0	0.0	31.63906550347479	0.0
21	0.0	0.0	0.0	32.82327717424394	0.0
22	0.0	0.0	0.0	34.13425994381192	0.0
23	0.0	0.0	0.0	35.3660923189326	0.0
24	0.0	0.0	0.0	36.3093736681424	0.0
25	0.0	0.0	0.0	37.14327960964069	0.0
26	0.0	0.0	0.0	37.899339833523236	0.0
27	0.0	0.0	0.0	38.61517252176636	0.0
28	0.0	0.0	0.0	39.38384462168721	0.0
29	0.0	0.0	0.0	40.16034478259735	0.0
30	0.0	0.0	0.0	41.002296231223525	0.0
31	0.0	0.0	0.0	41.762270485600716	0.0
32	0.0	0.0	0.0	42.51072009463256	0.0
33	0.0	0.0	0.0	43.23503318228075	0.0
34	0.0	0.0	0.0	43.97674196101626	0.0
35	0.0	0.0	0.0	44.686268711240224	0.0
36	0.0	0.0	0.0	45.38992441572223	0.0
37	2.174461385914709E-4	0.0	0.0	46.08879630515521	0.0
38	2.174461385914709E-4	0.0	0.0	46.845726313592124	0.0
39	2.174461385914709E-4	0.0	0.0	47.5704742935175	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAATTG	35	1.2098099E-7	45.000004	9
CGGGTGC	25	3.887144E-5	45.0	6
TGCCCCG	20	7.028867E-4	45.0	1
ATGATAG	20	7.028867E-4	45.0	11
GTCTGCG	40	6.7993824E-9	45.0	1
TGGGTAG	90	0.0	45.0	6
GCTATAG	25	3.887144E-5	45.0	14
ATTCGCC	20	7.028867E-4	45.0	40
TAACGAC	20	7.028867E-4	45.0	36
GCCGCGT	20	7.028867E-4	45.0	17
CTAAAGT	20	7.028867E-4	45.0	19
CATAACG	20	7.028867E-4	45.0	34
TCGACGG	20	7.028867E-4	45.0	2
ATCATAC	25	3.887144E-5	45.0	10
TACCGGG	45	3.8380676E-10	45.0	3
CTTACCG	20	7.028867E-4	45.0	1
CGAAGCA	20	7.028867E-4	45.0	29
TCGTGTA	30	2.162651E-6	44.999996	17
TTACCGG	30	2.162651E-6	44.999996	2
TACGGCT	1070	0.0	42.897194	7
>>END_MODULE