FastQCFastQC Report
Sat 18 Jun 2016
SRR3549573_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549573_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences724227
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGC111931.5455099022820193No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCG92281.2741861322485906No Hit
CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC86371.192581883856857No Hit
GAATCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC80151.1066972095765555No Hit
GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC69960.9659954682716884No Hit
CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT63780.8806631070092665Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC50270.6941193852203797No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTACTCGGT36310.501362141980346No Hit
GAACTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT23520.32476005451329487No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTACTCGGTCGTA22850.315508811463809No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTC20430.28209387388208396No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCT18930.2613821357115932No Hit
CGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG18350.25337359695233674No Hit
TGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCTT16550.22851951114774785Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16130.22272022446001047No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCC10850.14981490609988304No Hit
TTCCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT10030.13849248923334812No Hit
TCCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG9970.13766401970652847No Hit
TGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG9560.13200281127326102No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC8840.12206117695142546No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTACTCGGTCGT7930.10949605579466107No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGCGT351.2111013E-745.0000044
TAGTCGT351.2111013E-745.00000413
CATATCG207.0315565E-445.029
AGTTTCG253.8893722E-545.01
TCCCTCG207.0315565E-445.01
CGACCGA207.0315565E-445.040
CATTCGA207.0315565E-445.011
CGGGCTA207.0315565E-445.06
GCGATGC453.8380676E-1045.09
CGATGCG253.8893722E-545.010
TTGTGCG1200.043.1250041
TTCCACG750.042.01
CGGGTTC700.041.7857176
TACGGCT13950.041.7741937
TGATACG26300.041.6634984
GAATGAT39100.041.4322281
TACGGGA1250.041.3999984
ATACGGC26600.041.2781946
GATAGAC603.6379788E-1241.2500049
TTTTCCG3600.041.251
Produced by FastQC (version 0.11.5)