##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3549573_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 724227 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.84385696749776 31.0 25.0 33.0 16.0 34.0 2 29.13165071172436 31.0 28.0 34.0 16.0 34.0 3 29.933450423693124 31.0 30.0 34.0 16.0 34.0 4 33.114113392624134 35.0 32.0 37.0 28.0 37.0 5 34.0699173049334 35.0 33.0 37.0 30.0 37.0 6 33.573345097600615 35.0 33.0 37.0 28.0 37.0 7 33.791686860611385 35.0 33.0 36.0 30.0 37.0 8 34.147560088204386 35.0 35.0 37.0 30.0 37.0 9 34.97357458365954 37.0 34.0 39.0 29.0 39.0 10 34.022296876531804 35.0 32.0 39.0 27.0 39.0 11 34.622357354807264 37.0 33.0 39.0 27.0 39.0 12 34.682160427600735 37.0 34.0 39.0 27.0 39.0 13 35.11272018303654 37.0 34.0 39.0 30.0 39.0 14 34.629653409773454 37.0 33.0 39.0 26.0 40.0 15 35.199900031343766 37.0 33.0 39.0 27.0 40.0 16 34.82259429709193 36.0 32.0 39.0 27.0 40.0 17 34.66614749242986 36.0 32.0 39.0 27.0 40.0 18 34.623553112490974 37.0 32.0 39.0 27.0 40.0 19 34.466932329228264 37.0 32.0 39.0 27.0 40.0 20 35.652335524635234 37.0 34.0 39.0 30.0 40.0 21 34.72094799006389 37.0 33.0 39.0 26.0 40.0 22 35.57872738795985 37.0 34.0 39.0 29.0 40.0 23 36.49383549632919 38.0 35.0 40.0 31.0 40.0 24 36.57758133844775 38.0 35.0 40.0 31.0 41.0 25 34.391074897787576 37.0 32.0 39.0 25.0 40.0 26 35.00990711475822 37.0 33.0 39.0 27.0 40.0 27 35.82942917068819 37.0 34.0 39.0 30.0 40.0 28 35.98183718640703 38.0 35.0 40.0 30.0 40.0 29 36.54401451478611 38.0 35.0 40.0 30.0 41.0 30 34.42617439007383 37.0 33.0 39.0 24.0 40.0 31 34.957825377954705 37.0 33.0 39.0 27.0 40.0 32 35.62370776013598 37.0 34.0 39.0 30.0 40.0 33 36.495666413983464 38.0 35.0 40.0 31.0 40.0 34 36.71592470316627 38.0 35.0 40.0 31.0 41.0 35 36.94026320476867 39.0 36.0 40.0 32.0 41.0 36 36.792327543712126 39.0 35.0 40.0 31.0 41.0 37 36.9075358968942 39.0 35.0 40.0 31.0 41.0 38 36.43220564822908 38.0 35.0 40.0 30.0 41.0 39 36.44359986578794 38.0 35.0 40.0 31.0 41.0 40 35.98535265876583 38.0 34.0 40.0 30.0 41.0 41 35.57980301756217 38.0 34.0 40.0 29.0 41.0 42 36.43817339038727 38.0 35.0 40.0 31.0 41.0 43 36.41953696838146 38.0 35.0 40.0 31.0 41.0 44 36.748377235314344 39.0 35.0 40.0 31.0 41.0 45 37.06286979082525 39.0 36.0 40.0 32.0 41.0 46 36.89625214193892 39.0 35.0 40.0 32.0 41.0 47 36.83423705550884 39.0 35.0 40.0 31.0 41.0 48 36.817869259223976 39.0 35.0 40.0 32.0 41.0 49 36.947970732933186 39.0 35.0 40.0 32.0 41.0 50 36.7772121171953 39.0 35.0 40.0 32.0 41.0 51 35.842071615667464 38.0 34.0 40.0 30.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 7.0 10 11.0 11 13.0 12 18.0 13 21.0 14 16.0 15 43.0 16 71.0 17 162.0 18 234.0 19 426.0 20 691.0 21 924.0 22 1417.0 23 2093.0 24 2923.0 25 4161.0 26 5656.0 27 7632.0 28 10167.0 29 14041.0 30 19783.0 31 27857.0 32 39464.0 33 52728.0 34 70267.0 35 97342.0 36 129866.0 37 140779.0 38 85697.0 39 9717.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.008834246720987 3.978172589533392 51.64513336288208 18.367859800863542 2 18.19760931310211 9.863620108060042 51.893536142673504 20.045234436164353 3 18.482740908582528 9.75868063466289 52.65931814196377 19.099260314790804 4 17.72165356994423 3.6028758938841 56.204063090716026 22.47140744545564 5 21.14337079396377 4.623136116162474 50.399253272799825 23.83423981707393 6 20.56482290773473 9.883641454958184 51.39203592243868 18.159499714868403 7 77.26265383643526 1.333697859925134 16.318364269766246 5.085284033873357 8 76.09050753423996 6.0797236225658535 10.0489211255587 7.780847717635493 9 71.19342416120912 4.373352553826355 12.473161039287406 11.960062245677115 10 32.73145022209887 35.15306664899265 18.430271171883952 13.685211957024524 11 23.397912532948922 23.35786983915264 33.10343303964088 20.14078458825755 12 21.356839775374294 20.413903375599087 35.8023106015103 22.426946247516316 13 21.40834296429158 22.08382178515852 37.91628867744505 18.591546573104843 14 18.467828457099777 25.965753831326367 31.353843477252298 24.21257423432156 15 14.338184022412861 27.36572925339707 37.677137140703124 20.618949583486945 16 16.188156475801097 26.124819980475735 32.34855922245373 25.338464321269438 17 17.29609638966788 26.106041337867826 32.53372216169792 24.064140110766374 18 16.33686675586522 24.979737016156538 34.731375659841454 23.952020568136785 19 17.85296598994514 26.60353728872301 32.20426744653265 23.3392292747992 20 20.392501246156247 27.14135208988342 35.503233102328416 16.96291356163192 21 22.881637939485824 26.91504183080719 30.446393188875863 19.756927040831123 22 18.407626338150884 24.939003931087907 31.69448253102963 24.958887199731574 23 17.807400165970062 27.811445858826033 30.125361247233258 24.25579272797065 24 21.56851373947671 23.60268258432784 29.605496619153936 25.223307057041506 25 18.37186407024317 27.090263135729543 27.695598203325755 26.842274590701532 26 20.20637175913077 25.736682007160738 29.815651722457186 24.241294511251308 27 19.359261667957696 27.483233847950988 31.370689024297633 21.786815459793687 28 15.79849964168693 27.08322114475158 33.23695471171332 23.881324501848177 29 20.49813111082575 21.767622582422362 31.19298231079482 26.54126399595707 30 18.387190756489332 24.59560331222117 34.75719629342734 22.26000963786216 31 20.0978422511174 27.07631723202808 26.638056852340497 26.187783664514026 32 22.30819894867217 27.316987629569184 26.993332201091647 23.381481220667 33 20.637866304349327 23.906592822416176 30.050798989819487 25.404741883415006 34 22.24399256034365 23.008531855343698 28.674849184026556 26.072626400286097 35 22.53326650345817 21.573484556637627 28.526138903962433 27.36711003594177 36 24.473956370033154 24.08402337941005 29.49655287637716 21.945467374179643 37 17.81347560916674 25.512304843647087 33.86382998700684 22.81038956017934 38 19.146068843056113 27.41378048595261 28.885832756856622 24.554317914134657 39 20.730792969607595 21.847017578742577 31.03778235277061 26.38440709887922 40 22.46201812415168 20.65526416441254 32.735316413224034 24.147401298211747 41 22.525534121207855 23.305262024199592 27.000650348578553 27.168553506014 42 25.143773982466822 21.54614506225258 29.323816980035268 23.986263975245333 43 26.073178713303978 21.452113770958555 28.292234340890353 24.182473174847114 44 19.82403307250351 21.93276417476841 31.43517156913509 26.80803118359299 45 20.229430827627247 20.101570363988085 29.419781366891872 30.249217441492792 46 23.80883341825146 21.68104751686971 30.94651262656598 23.56360643831285 47 17.246112061549763 21.427121606899494 37.22189313571573 24.104873195835008 48 19.591647370230607 22.311927061542857 30.74850840965609 27.34791715857045 49 20.932801455897117 19.35277198999761 35.627365453096886 24.087061101008388 50 23.081299095449356 18.306967290642298 30.555336931652644 28.056396682255702 51 19.474833166949036 18.201475504227265 28.471045680428926 33.85264564839477 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 110.0 1 351.5 2 593.0 3 1793.5 4 2994.0 5 2171.5 6 1349.0 7 1348.5 8 1348.0 9 1391.0 10 1434.0 11 1567.5 12 1701.0 13 1762.5 14 1824.0 15 1840.5 16 1857.0 17 1969.0 18 2081.0 19 2200.5 20 2320.0 21 2694.5 22 3069.0 23 3555.0 24 4041.0 25 4809.0 26 7151.5 27 8726.0 28 9959.0 29 11192.0 30 12105.5 31 13019.0 32 15179.5 33 17340.0 34 18876.5 35 20413.0 36 22444.5 37 24476.0 38 26048.5 39 27621.0 40 29307.5 41 30994.0 42 33830.5 43 36667.0 44 39739.5 45 42812.0 46 47341.5 47 51871.0 48 80170.5 49 108470.0 50 103201.0 51 97932.0 52 90674.0 53 83416.0 54 62862.5 55 42309.0 56 33576.0 57 24843.0 58 21355.0 59 17867.0 60 15462.5 61 13058.0 62 10816.0 63 8574.0 64 6977.5 65 5381.0 66 4140.5 67 2900.0 68 2368.0 69 1836.0 70 1378.0 71 920.0 72 742.0 73 564.0 74 456.0 75 279.0 76 210.0 77 147.0 78 84.0 79 77.0 80 70.0 81 40.0 82 10.0 83 6.0 84 2.0 85 2.5 86 3.0 87 1.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 724227.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.529793678122815 #Duplication Level Percentage of deduplicated Percentage of total 1 68.8233300363082 23.07632056358767 2 13.170689756077406 8.832210202396823 3 5.260177053482757 5.2911795394101855 4 2.595507670795052 3.4810734676697286 5 1.6394475601955925 2.748516921973003 6 1.0733157277386414 2.1592832941536524 7 0.8128340607337532 1.9077910845667445 8 0.6736002840693666 1.8068542837096622 9 0.5696695142932177 1.7190811151071221 >10 5.189111948636298 30.72850033235932 >50 0.11572284814709616 2.665588387028596 >100 0.06410546242655195 3.909334220314388 >500 0.005411500074968671 1.2244845983368964 >1k 0.004162692365360516 2.7070419223463014 >5k 0.0024976154192163096 6.180485865605392 >10k+ 4.162692365360516E-4 1.5622542014345013 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGC 11193 1.5455099022820193 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCG 9228 1.2741861322485906 No Hit CCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC 8637 1.192581883856857 No Hit GAATCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC 8015 1.1066972095765555 No Hit GCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC 6996 0.9659954682716884 No Hit CTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCT 6378 0.8806631070092665 Illumina Single End Adapter 2 (95% over 21bp) TCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGC 5027 0.6941193852203797 No Hit GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTACTCGGT 3631 0.501362141980346 No Hit GAACTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT 2352 0.32476005451329487 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTACTCGGTCGTA 2285 0.315508811463809 No Hit GAATGATCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTC 2043 0.28209387388208396 No Hit GAATGACTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCT 1893 0.2613821357115932 No Hit CGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG 1835 0.25337359695233674 No Hit TGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTGCTT 1655 0.22851951114774785 Illumina Single End Adapter 2 (95% over 22bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1613 0.22272022446001047 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCC 1085 0.14981490609988304 No Hit TTCCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCT 1003 0.13849248923334812 No Hit TCCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG 997 0.13766401970652847 No Hit TGCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTCTG 956 0.13200281127326102 No Hit CGTTCTGTCTCTTATACACATCTGACGCGTACTCGGTCGTATGCCGTCTTC 884 0.12206117695142546 No Hit GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTACTCGGTCGT 793 0.10949605579466107 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.9922303366209766 0.0 2 0.0 0.0 0.0 4.101338392520577 0.0 3 0.0 0.0 0.0 5.123255553852591 0.0 4 0.0 0.0 0.0 6.7244109926859945 0.0 5 0.0 0.0 0.0 9.763789530078277 0.0 6 0.0 0.0 0.0 10.690708852334973 0.0 7 0.0 0.0 0.0 11.939902820524503 0.0 8 0.0 0.0 0.0 13.615896673280615 0.0 9 0.0 0.0 0.0 14.239596148721326 0.0 10 0.0 0.0 0.0 16.82925381130502 0.0 11 0.0 0.0 0.0 19.387843866632977 0.0 12 0.0 0.0 0.0 23.32114102346364 0.0 13 0.0 0.0 0.0 24.14753937646622 0.0 14 0.0 0.0 0.0 24.45835352727805 0.0 15 0.0 0.0 0.0 25.183126284990756 0.0 16 0.0 0.0 0.0 26.120401476332695 0.0 17 0.0 0.0 0.0 27.531008923997586 0.0 18 0.0 0.0 0.0 28.914000720768488 0.0 19 0.0 0.0 0.0 30.51847003770917 0.0 20 0.0 0.0 0.0 31.700696052480783 0.0 21 0.0 0.0 0.0 33.085897101323205 0.0 22 0.0 0.0 0.0 34.762719423606136 0.0 23 0.0 0.0 0.0 36.22911048607688 0.0 24 0.0 0.0 0.0 37.385653945517085 0.0 25 2.761565089398766E-4 0.0 0.0 38.34074123168565 0.0 26 2.761565089398766E-4 0.0 0.0 39.20442071339511 0.0 27 2.761565089398766E-4 0.0 0.0 40.03868952690247 0.0 28 4.142347634098149E-4 0.0 0.0 40.90278324337535 0.0 29 4.142347634098149E-4 0.0 0.0 41.7954591585235 0.0 30 4.142347634098149E-4 0.0 0.0 42.7871371821266 0.0 31 4.142347634098149E-4 0.0 0.0 43.69638248781114 0.0 32 4.142347634098149E-4 0.0 0.0 44.54100717040375 0.0 33 4.142347634098149E-4 0.0 0.0 45.30720340445744 0.0 34 4.142347634098149E-4 0.0 0.0 46.083893585850845 0.0 35 4.142347634098149E-4 0.0 0.0 46.893584470062564 0.0 36 4.142347634098149E-4 0.0 0.0 47.722053996882195 0.0 37 4.142347634098149E-4 0.0 0.0 48.543895767487264 0.0 38 4.142347634098149E-4 0.0 0.0 49.328594487639926 0.0 39 4.142347634098149E-4 0.0 0.0 50.13234800690944 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGGCGT 35 1.2111013E-7 45.000004 4 TAGTCGT 35 1.2111013E-7 45.000004 13 CATATCG 20 7.0315565E-4 45.0 29 AGTTTCG 25 3.8893722E-5 45.0 1 TCCCTCG 20 7.0315565E-4 45.0 1 CGACCGA 20 7.0315565E-4 45.0 40 CATTCGA 20 7.0315565E-4 45.0 11 CGGGCTA 20 7.0315565E-4 45.0 6 GCGATGC 45 3.8380676E-10 45.0 9 CGATGCG 25 3.8893722E-5 45.0 10 TTGTGCG 120 0.0 43.125004 1 TTCCACG 75 0.0 42.0 1 CGGGTTC 70 0.0 41.785717 6 TACGGCT 1395 0.0 41.774193 7 TGATACG 2630 0.0 41.663498 4 GAATGAT 3910 0.0 41.432228 1 TACGGGA 125 0.0 41.399998 4 ATACGGC 2660 0.0 41.278194 6 GATAGAC 60 3.6379788E-12 41.250004 9 TTTTCCG 360 0.0 41.25 1 >>END_MODULE