FastQCFastQC Report
Sat 18 Jun 2016
SRR3549570_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549570_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804099
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGC228231.265063613471323No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCG181801.007705231253939No Hit
GAATCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTC166840.9247829525984993No Hit
GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC88640.49132558689961026TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACAATCCCT67950.3766423017805564No Hit
CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC61150.33895035693717473TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59230.3283079254519846No Hit
CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT56630.31389629948245634TruSeq Adapter, Index 13 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT47210.26168187000824233No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACAATCCCTCGTA40060.22204989859203958No Hit
TCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC38260.21207261907467387TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTC36120.20021074231513902No Hit
GAATGACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT32320.17914759666736693No Hit
CGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG20670.11457242645774983No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCC18670.10348656032734345No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTAG207.034355E-445.00000427
TACGGCT28150.042.5222057
ATGATAC81700.040.3733183
CGTTTTT35350.040.099011
TGATACG60900.040.0123184
ATACGGC60600.039.8762366
TTTCGCG6500.039.8076931
GATACGG61000.039.725415
GAATGAT85900.039.525611
TGATACC22300.039.450674
TTGGGAC38800.039.432995
ACCGGTA800.039.37500441
AATGATA83000.039.280122
TTCGGGA15200.039.0789454
GATACCT22550.039.0133065
ACGGCTG30750.038.9268268
GCGATTA1000.038.259
TTTGGGA93050.038.1810844
CATACGA2300.038.15217218
TTTGGGC41700.037.7697874
Produced by FastQC (version 0.11.5)