##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3549570_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1804099 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.886507336903353 31.0 25.0 33.0 16.0 34.0 2 29.25043913887209 31.0 28.0 34.0 16.0 34.0 3 30.1519811274215 31.0 30.0 34.0 25.0 34.0 4 33.13568989284956 35.0 32.0 37.0 28.0 37.0 5 33.969314876844344 35.0 33.0 37.0 28.0 37.0 6 33.6396760931634 35.0 35.0 37.0 28.0 37.0 7 33.4811066355006 35.0 32.0 35.0 29.0 37.0 8 34.16834220295006 35.0 35.0 37.0 30.0 37.0 9 34.90754886511217 37.0 34.0 39.0 29.0 39.0 10 33.32255713239684 35.0 31.0 39.0 27.0 39.0 11 33.83472525620822 35.0 32.0 39.0 27.0 39.0 12 34.04434512740154 37.0 32.0 39.0 26.0 39.0 13 34.48557035949801 37.0 33.0 39.0 27.0 39.0 14 34.58542962442748 37.0 33.0 39.0 25.0 40.0 15 34.934925411521206 37.0 32.0 39.0 27.0 40.0 16 34.66868614194676 36.0 32.0 39.0 27.0 40.0 17 34.51107062306448 36.0 32.0 39.0 27.0 40.0 18 34.43196077377129 37.0 32.0 39.0 27.0 40.0 19 34.13239351055569 37.0 32.0 39.0 25.0 40.0 20 35.29251055513029 37.0 34.0 39.0 29.0 40.0 21 34.487095774677556 37.0 32.0 39.0 25.0 40.0 22 35.32644328276885 37.0 34.0 39.0 27.0 40.0 23 36.31378599511446 38.0 34.0 40.0 31.0 40.0 24 36.420590555174634 38.0 35.0 40.0 31.0 41.0 25 34.26677139114871 37.0 32.0 39.0 24.0 40.0 26 34.91122105826787 37.0 33.0 39.0 27.0 40.0 27 35.600057424786556 37.0 34.0 39.0 30.0 40.0 28 35.74109181369759 38.0 34.0 40.0 30.0 41.0 29 36.24526093080258 38.0 35.0 40.0 30.0 41.0 30 34.02138907011201 37.0 32.0 39.0 24.0 40.0 31 34.514434074848445 37.0 32.0 39.0 25.0 40.0 32 35.04413394165176 37.0 33.0 39.0 28.0 40.0 33 36.00293941740448 38.0 35.0 40.0 30.0 40.0 34 36.27404150215703 38.0 35.0 40.0 30.0 41.0 35 36.41076293485003 38.0 35.0 40.0 31.0 41.0 36 36.36818434021636 38.0 35.0 40.0 31.0 41.0 37 36.516872965397134 38.0 35.0 40.0 31.0 41.0 38 36.00112632399885 38.0 35.0 40.0 30.0 41.0 39 35.970767125307425 38.0 35.0 40.0 30.0 41.0 40 35.51801536390187 38.0 34.0 40.0 29.0 41.0 41 35.080882479287446 37.0 33.0 40.0 28.0 41.0 42 35.942490406568595 38.0 35.0 40.0 30.0 41.0 43 36.025882725947966 38.0 35.0 40.0 30.0 41.0 44 36.434654639240975 38.0 35.0 40.0 31.0 41.0 45 36.64894387724842 39.0 35.0 40.0 32.0 41.0 46 36.44576267710364 38.0 35.0 40.0 31.0 41.0 47 36.391201924062926 38.0 35.0 40.0 31.0 41.0 48 36.368528002066405 38.0 35.0 40.0 31.0 41.0 49 36.53675103195556 38.0 35.0 40.0 32.0 41.0 50 36.42888333733348 38.0 35.0 40.0 31.0 41.0 51 35.732243075352294 37.0 34.0 40.0 30.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 3.0 9 22.0 10 33.0 11 61.0 12 47.0 13 78.0 14 107.0 15 165.0 16 284.0 17 478.0 18 990.0 19 1646.0 20 2610.0 21 3623.0 22 5076.0 23 6754.0 24 9538.0 25 13180.0 26 17789.0 27 23377.0 28 30587.0 29 41840.0 30 56897.0 31 79372.0 32 109593.0 33 143554.0 34 177575.0 35 234545.0 36 295813.0 37 319555.0 38 203795.0 39 25112.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 25.88660600111191 3.8084384504398043 52.45371789463882 17.851237653809466 2 18.51638962163385 8.907493435781518 52.134334091421806 20.441782851162824 3 19.794977991784265 8.700797461780091 50.866277294095276 20.637947252340368 4 17.891590206524143 4.125549651100078 53.8524216243122 24.13043851806359 5 21.615776074372857 4.873734756241204 49.29845867660256 24.21203049278338 6 21.691547969374188 9.325153442244577 51.07247440412084 17.9108241842604 7 80.9108591047387 1.6585010024394449 13.488062462204125 3.942577430617721 8 81.80338218689772 5.5057954136663225 7.83571189829383 4.855110501142121 9 76.97083142333098 4.002718254375175 9.284246596223378 9.742203726070466 10 40.978682433724536 26.93992957149247 16.590885533443565 15.490502461339428 11 28.85218604965692 22.981333064316313 27.069855922540835 21.096624963485926 12 25.481417594045556 19.456969933468173 31.87136626094244 23.190246211543826 13 24.797087077815576 20.026340017925843 34.987658659530325 20.188914244728252 14 21.37194244883457 22.546434536020474 32.17644929685123 23.905173718293728 15 17.64049533867044 23.63922378982528 36.814997403135855 21.905283468368424 16 22.17672090057142 23.49571725276717 29.933612290678063 24.393949555983347 17 22.533020638002682 23.89314555354224 30.33924413238963 23.234589676065447 18 21.64548619560235 22.887324919530467 31.799086413772194 23.66810247109499 19 22.53241091536551 24.67281451849372 29.003729839659577 23.791044726481196 20 23.417173891233244 25.986212508293615 31.247010280477955 19.34960331999519 21 25.10898792139456 25.578363493355965 29.559242591454236 19.75340599379524 22 23.063423903011973 22.06181589812976 30.812887762811243 24.061872436047025 23 22.139250673050647 24.63063279786752 30.041422338796263 23.188694190285567 24 23.216408855611583 23.04823626641332 28.75213610783 24.983218770145097 25 21.93366328566226 26.026731349000247 26.44112102495484 25.598484340382647 26 20.889485554839286 25.71410992412279 27.600868910187305 25.795535610850624 27 20.949404661274134 25.604803284076983 28.586901273156297 24.85889078149259 28 18.47853138879851 26.312524977842127 30.293459505271052 24.915484128088313 29 20.87463049422454 25.671983632827246 28.077949159109338 25.375436713838873 30 21.444000578682214 24.544772764687526 29.561459764680322 24.449766891949942 31 22.04114075779655 26.27522103831331 26.4987120995023 25.184926104387838 32 22.081936745156447 28.464180735092697 25.64188550628319 23.811997013467664 33 21.376875659262602 24.095407181091502 26.87191778278243 27.655799376863467 34 20.99845961890118 26.060820387351246 27.562290096053488 25.378429897694083 35 19.206928222896856 25.828349774596624 26.349607200048336 28.61511480245818 36 20.182983306348486 27.935939213978834 25.75102585833704 26.13005162133564 37 19.287910474979476 27.568941615731728 27.200780001540934 25.942367907747855 38 20.152885179804432 28.059047757356996 26.929065422684673 24.859001640153895 39 20.911768145761403 25.94574909691763 27.37238920923963 25.77009354808134 40 21.182651284657883 22.893533004563498 29.56334436192249 26.360471348856134 41 19.05555072088616 24.22028946305053 26.554363147476938 30.16979666858637 42 19.241183549239814 23.640609523091584 28.37039430762946 28.74781262003914 43 20.7793474748337 23.865763464200136 27.247229780627336 28.107659280338826 44 20.72153468296363 23.41451328336194 28.52459870550341 27.33935332817102 45 20.607461120481748 22.708288181524406 27.70125142799813 28.982999269995712 46 22.269509600082923 24.77657822547432 27.522935271290542 25.430976903152207 47 17.6612259083343 24.353208997954106 32.560297411616546 25.425267682095054 48 19.039531644327724 25.088257351730697 28.422331590450412 27.449879413491168 49 20.014422711835657 22.746977854319525 31.34960997151487 25.88898946232995 50 20.534793267996935 22.61394746075465 28.48108668094157 28.37017259030685 51 18.8888747236155 22.590944288534054 27.306539164425015 31.21364182342543 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 284.0 1 1036.5 2 1789.0 3 6353.5 4 10918.0 5 7591.0 6 4264.0 7 4078.5 8 3893.0 9 3870.5 10 3848.0 11 3769.5 12 3691.0 13 3656.0 14 3621.0 15 3466.5 16 3312.0 17 3457.5 18 3603.0 19 4020.0 20 4437.0 21 4619.0 22 4801.0 23 5857.5 24 6914.0 25 8399.5 26 12287.0 27 14689.0 28 17838.0 29 20987.0 30 24461.0 31 27935.0 32 30816.0 33 33697.0 34 38419.5 35 43142.0 36 47775.0 37 52408.0 38 58147.0 39 63886.0 40 70233.0 41 76580.0 42 81307.5 43 86035.0 44 94994.5 45 103954.0 46 155478.0 47 207002.0 48 194940.0 49 182878.0 50 178765.0 51 174652.0 52 150588.5 53 126525.0 54 115545.0 55 104565.0 56 97221.0 57 89877.0 58 85670.5 59 81464.0 60 75766.5 61 70069.0 62 63104.5 63 56140.0 64 49530.5 65 42921.0 66 36486.0 67 30051.0 68 24674.0 69 19297.0 70 15973.0 71 12649.0 72 10246.0 73 7843.0 74 6366.5 75 3722.0 76 2554.0 77 1953.5 78 1353.0 79 929.0 80 505.0 81 337.0 82 169.0 83 106.0 84 43.0 85 45.5 86 48.0 87 38.5 88 29.0 89 15.0 90 1.0 91 0.5 92 0.0 93 0.5 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1804099.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.99803779382617 #Duplication Level Percentage of deduplicated Percentage of total 1 71.55833068252755 20.750511775950454 2 11.262102950147199 6.531577739726593 3 4.100238236643929 3.5669659004967547 4 2.033974276884043 2.3592505181101493 5 1.1623807680478158 1.6853380721333626 6 0.7778331522550707 1.3533381087830092 7 0.6512642706048171 1.3219770152936985 8 0.5035298882709738 1.1681102984322214 9 0.4276664828352315 1.116133994916788 >10 7.313937173687428 46.797262538859705 >50 0.1451779558543223 2.7559390459705657 >100 0.05613206293142527 2.9373218367727434 >500 0.0033248865335178267 0.7063830197929466 >1k 0.0025425602903371616 1.7861763810003262 >5k 9.779078039758315E-4 1.8920062256357044 >10k+ 5.867446823854988E-4 3.271707528124907 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGC 22823 1.265063613471323 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCG 18180 1.007705231253939 No Hit GAATCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTC 16684 0.9247829525984993 No Hit GCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC 8864 0.49132558689961026 TruSeq Adapter, Index 19 (95% over 21bp) GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCACAATCCCT 6795 0.3766423017805564 No Hit CCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC 6115 0.33895035693717473 TruSeq Adapter, Index 19 (95% over 21bp) CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5923 0.3283079254519846 No Hit CTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGCT 5663 0.31389629948245634 TruSeq Adapter, Index 13 (95% over 22bp) GAACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCT 4721 0.26168187000824233 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCACAATCCCTCGTA 4006 0.22204989859203958 No Hit TCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTGC 3826 0.21207261907467387 TruSeq Adapter, Index 19 (95% over 21bp) GAATGATCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTC 3612 0.20021074231513902 No Hit GAATGACTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCT 3232 0.17914759666736693 No Hit CGCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCCGTCTTCTG 2067 0.11457242645774983 No Hit CGTTTTTTTTCTGTCTCTTATACACATCTGACGCACAATCCCTCGTATGCC 1867 0.10348656032734345 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.3652792889968899 0.0 2 0.0 0.0 0.0 1.5164910573089392 0.0 3 0.0 0.0 0.0 1.9588725452428055 0.0 4 0.0 0.0 0.0 2.790367934353935 0.0 5 0.0 0.0 0.0 4.65318144957677 0.0 6 0.0 0.0 0.0 5.385790912804675 0.0 7 0.0 0.0 0.0 6.2035398279141 0.0 8 0.0 0.0 0.0 7.257306832939878 0.0 9 0.0 0.0 0.0 7.684001820299218 0.0 10 0.0 0.0 0.0 9.516107486340827 0.0 11 0.0 0.0 0.0 11.019184645631976 0.0 12 0.0 0.0 0.0 13.63090384729441 0.0 13 0.0 0.0 0.0 14.16341342686848 0.0 14 0.0 0.0 0.0 14.37886723511293 0.0 15 0.0 0.0 0.0 14.866257339536245 0.0 16 0.0 0.0 0.0 15.406582454732252 0.0 17 0.0 0.0 0.0 16.136365022096903 0.0 18 0.0 0.0 0.0 16.847855910346382 0.0 19 0.0 0.0 0.0 17.83510771858972 0.0 20 5.5429330652031845E-5 0.0 0.0 18.51406158974646 0.0 21 5.5429330652031845E-5 0.0 0.0 19.38319349437032 0.0 22 5.5429330652031845E-5 0.0 0.0 20.33713227489179 0.0 23 5.5429330652031845E-5 0.0 0.0 21.22161810410626 0.0 24 5.5429330652031845E-5 0.0 0.0 21.921579691580117 0.0 25 5.5429330652031845E-5 0.0 0.0 22.53917329370506 0.0 26 5.5429330652031845E-5 0.0 0.0 23.125116748027686 0.0 27 5.5429330652031845E-5 0.0 0.0 23.706681285228804 0.0 28 1.1085866130406369E-4 0.0 0.0 24.276328516339735 0.0 29 1.1085866130406369E-4 0.0 0.0 24.89164951590794 0.0 30 1.1085866130406369E-4 0.0 0.0 25.58263155181617 0.0 31 1.1085866130406369E-4 0.0 0.0 26.18553638131832 0.0 32 1.1085866130406369E-4 0.0 0.0 26.76089283348641 0.0 33 1.1085866130406369E-4 0.0 0.0 27.32505256086279 0.0 34 1.1085866130406369E-4 0.0 0.0 27.9145989216778 0.0 35 1.1085866130406369E-4 0.0 0.0 28.55264594681334 0.0 36 1.1085866130406369E-4 0.0 0.0 29.13731452653097 0.0 37 1.1085866130406369E-4 0.0 0.0 29.744376555832023 0.0 38 1.1085866130406369E-4 0.0 0.0 30.395671190993397 0.0 39 1.1085866130406369E-4 0.0 0.0 31.249726317679904 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTTAG 20 7.034355E-4 45.000004 27 TACGGCT 2815 0.0 42.522205 7 ATGATAC 8170 0.0 40.373318 3 CGTTTTT 3535 0.0 40.09901 1 TGATACG 6090 0.0 40.012318 4 ATACGGC 6060 0.0 39.876236 6 TTTCGCG 650 0.0 39.807693 1 GATACGG 6100 0.0 39.72541 5 GAATGAT 8590 0.0 39.52561 1 TGATACC 2230 0.0 39.45067 4 TTGGGAC 3880 0.0 39.43299 5 ACCGGTA 80 0.0 39.375004 41 AATGATA 8300 0.0 39.28012 2 TTCGGGA 1520 0.0 39.078945 4 GATACCT 2255 0.0 39.013306 5 ACGGCTG 3075 0.0 38.926826 8 GCGATTA 100 0.0 38.25 9 TTTGGGA 9305 0.0 38.181084 4 CATACGA 230 0.0 38.152172 18 TTTGGGC 4170 0.0 37.769787 4 >>END_MODULE