FastQCFastQC Report
Sat 18 Jun 2016
SRR3549569_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549569_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences713320
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGC208702.9257556216004037No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCG152082.132002467335838No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC130481.8291930690293274No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGGACTCT63650.8923063982504346No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC60980.8548757920708798TruSeq Adapter, Index 13 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT44420.6227219200358884TruSeq Adapter, Index 8 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTA42270.5925811697414904No Hit
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC31380.43991476476195823TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTC31250.438092300790669No Hit
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT27600.38692312005831886TruSeq Adapter, Index 8 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCT26370.3696798070991981No Hit
TCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC20980.29411764705882354TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGT15280.21420961139460548No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATG12730.17846127965008693No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12680.17776033196882185No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTAT8890.12462849772892952No Hit
TGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCTT8520.119441484887568TruSeq Adapter, Index 13 (96% over 25bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCAGGACTC7140.10009532888465204No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT1100.045.0000046
TCGCAAT207.0314854E-445.013
AGCGATA207.0314854E-445.039
TTGCGAT207.0314854E-445.027
ACGTCGT207.0314854E-445.021
CGTAACC253.8893126E-545.035
ACGTACC207.0314854E-445.033
ATACGTT253.8893126E-545.027
CGCAATA207.0314854E-445.014
GCTCGTA253.8893126E-545.032
AACGTCG302.164339E-644.99999620
CGAATCG302.164339E-644.99999616
TACGGCT22950.043.3333367
TACGGGG1300.043.2692344
ACGGCTG23500.042.319158
AGACACG1250.041.39999824
ATGATAC68900.041.3425253
ATACGAA603.6379788E-1241.24999619
ATACGGC49800.041.204826
CTTTGCG1750.041.1428571
Produced by FastQC (version 0.11.5)