##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549569_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	713320
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.938950260752538	31.0	16.0	33.0	16.0	34.0
2	28.209360455335613	31.0	25.0	33.0	16.0	34.0
3	29.192865754500083	31.0	28.0	34.0	16.0	34.0
4	32.588023607917904	35.0	32.0	37.0	28.0	37.0
5	33.765545617675095	35.0	33.0	37.0	28.0	37.0
6	33.00213368474177	35.0	33.0	37.0	26.0	37.0
7	33.4216326473392	35.0	32.0	35.0	29.0	37.0
8	33.57273173330343	35.0	35.0	37.0	28.0	37.0
9	34.705404306622555	37.0	32.0	39.0	28.0	39.0
10	33.35941372735939	35.0	31.0	38.0	27.0	39.0
11	33.739274098581284	35.0	32.0	39.0	27.0	39.0
12	34.08483149217742	37.0	32.0	39.0	26.0	39.0
13	34.403330903381374	35.0	33.0	39.0	27.0	39.0
14	34.25871418157349	37.0	32.0	39.0	25.0	40.0
15	34.77705658049683	37.0	32.0	39.0	27.0	40.0
16	34.490537206302925	36.0	32.0	39.0	27.0	40.0
17	34.25938428755678	36.0	32.0	38.0	27.0	40.0
18	34.27576122918185	36.0	32.0	39.0	27.0	40.0
19	34.13131273481747	36.0	32.0	39.0	25.0	40.0
20	34.945449447653225	37.0	33.0	39.0	27.0	40.0
21	34.27884680087478	37.0	32.0	39.0	25.0	40.0
22	35.3111268434924	37.0	34.0	39.0	27.0	40.0
23	36.0410432905288	38.0	34.0	39.0	30.0	40.0
24	36.15218835866091	38.0	35.0	40.0	30.0	40.0
25	34.03518476980878	37.0	32.0	39.0	24.0	40.0
26	34.80318370436831	37.0	33.0	39.0	27.0	40.0
27	35.31792603600067	37.0	34.0	39.0	29.0	40.0
28	35.455796837324066	38.0	34.0	39.0	29.0	40.0
29	36.00321034038019	38.0	34.0	40.0	30.0	41.0
30	33.6016948914933	36.0	31.0	39.0	23.0	40.0
31	34.483819323725676	36.0	32.0	39.0	25.0	40.0
32	34.90499635507206	37.0	33.0	39.0	28.0	40.0
33	35.705376268715305	38.0	34.0	39.0	30.0	40.0
34	36.11982700611227	38.0	35.0	40.0	30.0	41.0
35	36.252056580496834	38.0	35.0	40.0	30.0	41.0
36	36.2189199798127	38.0	35.0	40.0	30.0	41.0
37	36.47267846127965	38.0	35.0	40.0	31.0	41.0
38	36.000368698480344	38.0	35.0	40.0	30.0	41.0
39	35.96089833454831	38.0	35.0	40.0	30.0	41.0
40	35.38211461896484	38.0	34.0	40.0	28.0	41.0
41	35.11963074076151	37.0	33.0	40.0	28.0	41.0
42	36.00744686816576	38.0	35.0	40.0	30.0	41.0
43	36.15078085571693	38.0	35.0	40.0	31.0	41.0
44	36.5680073459317	38.0	35.0	40.0	31.0	41.0
45	36.834309987102564	39.0	35.0	40.0	32.0	41.0
46	36.534202041159645	38.0	35.0	40.0	31.0	41.0
47	36.407421634049236	38.0	35.0	40.0	31.0	41.0
48	36.464100263556325	38.0	35.0	40.0	31.0	41.0
49	36.694357371165815	39.0	35.0	40.0	32.0	41.0
50	36.50895390568048	38.0	35.0	40.0	31.0	41.0
51	35.89467139572702	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	6.0
10	11.0
11	9.0
12	17.0
13	26.0
14	34.0
15	67.0
16	120.0
17	203.0
18	432.0
19	839.0
20	1335.0
21	1907.0
22	2284.0
23	2820.0
24	3655.0
25	5321.0
26	7047.0
27	9592.0
28	12782.0
29	17509.0
30	24230.0
31	34329.0
32	47016.0
33	60923.0
34	74001.0
35	93599.0
36	113399.0
37	118838.0
38	72810.0
39	8158.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.49256995457859	3.5552066393764368	47.650984130544494	16.301239275500475
2	17.405792631637976	15.740621320024673	47.33597824258398	19.51760780575338
3	18.086973588291368	15.546599001850502	47.32322099478495	19.04320641507318
4	17.10242247518645	3.7321258341277406	56.49764481579095	22.667806874894858
5	26.32268827454719	4.571300398138283	45.81884708125386	23.287164246060673
6	20.08873997644816	14.549430830482812	48.00384119329333	17.3579879997757
7	74.46433578197723	1.5775528514551673	19.674620086356754	4.283491280210845
8	74.30844501766389	10.566926484607189	8.81021140582067	6.31441709190826
9	70.04191667133965	3.869651769191947	9.896680311781529	16.191751247686874
10	39.94462513318006	23.72876128525767	19.230639825043458	17.095973756518816
11	32.637666124600464	23.786799753266415	24.303117815286267	19.272416306846857
12	26.318762967532106	19.037598833623058	29.7839679246341	24.859670274210732
13	25.602674816351705	21.103852408456234	34.795884035215614	18.497588739976447
14	21.995457859025404	26.054786070767676	27.98141086749285	23.96834520271407
15	17.26321987326866	23.52464532047328	37.6814052599114	21.53072954634666
16	19.11960971233107	22.691498906521616	29.428587450232712	28.7603039309146
17	19.04923456513206	24.821118151741153	32.999635507205745	23.130011775921044
18	19.192227892110132	23.01435540851231	31.812931082823976	25.98048561655358
19	19.83541748443896	26.689564290921325	29.08736611899288	24.387652105646833
20	23.333566982560423	25.316127404250548	33.62291818538664	17.727387427802388
21	24.193209218863903	28.252116861997422	27.13578758481467	20.418886334324007
22	20.615151685078224	22.89477373408849	30.69576066842371	25.794313912409578
23	20.865390007289854	28.889138114731118	28.05388885773566	22.191583020243367
24	23.129170638703528	22.855100095328883	27.331071608815115	26.68465765715247
25	18.724835978242584	29.636067963887175	24.850978522963047	26.788117534907197
26	19.48662591824146	24.072786407222566	28.713060057197332	27.727527617338644
27	21.09389895138227	28.27819211574048	26.248948578478103	24.378960354399148
28	18.697078450064485	25.462625469634947	31.5419447092469	24.298351371053663
29	23.0487018448943	22.8186508159031	26.348483149217746	27.78416418998486
30	21.37638086693209	29.158862782481915	28.249313071272358	21.215443279313632
31	25.794734481018338	24.013626422923792	23.747266304043066	26.444372792014804
32	24.553636516570403	29.215919923736895	25.158834744574666	21.07160881511804
33	20.996327034150173	25.828379969719062	23.47529860371222	29.69999439241855
34	23.7883418381652	23.45062524533169	25.52515000280379	27.23588291369932
35	19.572421914428308	25.454214097459765	26.931531430494026	28.0418325576179
36	21.460354399147647	25.66365726462177	23.525486457690796	29.350501878539788
37	22.130600571973307	27.831408063702124	28.190433466046095	21.84755789827847
38	25.621600403745866	27.125834127740706	23.704368305949643	23.548197162563785
39	25.49641114787192	23.14613357259014	26.08520720013458	25.272248079403354
40	20.154629058487075	26.783491280210846	29.27507990803566	23.786799753266415
41	18.81175349071945	24.178489317557336	25.676975270565805	31.332781921157405
42	19.91630684685695	21.823725677115462	32.24709807659956	26.01286939942803
43	22.473363988111927	21.840127852857062	25.16780687489486	30.518701284136153
44	21.23633152021533	22.819491953120618	28.185807211349744	27.758369315314308
45	22.063870352716876	20.776650030841697	26.63531094039141	30.524168676050017
46	25.795996186844615	24.373633152021533	25.605758986149276	24.22461167498458
47	17.57626310772164	23.799276621992934	34.84382885661414	23.780631413671284
48	21.036281051982282	24.322043402680425	27.003729041664332	27.637946503672968
49	20.653143049402793	20.487579207087983	34.059748780351036	24.799528963158192
50	22.45962541355913	20.21897605562721	28.85479167834913	28.466606852464533
51	19.436578253799137	20.86370773285482	26.493298940167104	33.20641507317894
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	46.0
1	393.0
2	740.0
3	1649.5
4	2559.0
5	1853.5
6	1148.0
7	1123.5
8	1099.0
9	1193.0
10	1287.0
11	1252.5
12	1218.0
13	1209.5
14	1201.0
15	1172.0
16	1143.0
17	1135.0
18	1127.0
19	1397.0
20	1667.0
21	1593.5
22	1520.0
23	1969.0
24	2418.0
25	3046.5
26	4727.0
27	5779.0
28	6710.0
29	7641.0
30	8351.0
31	9061.0
32	10969.0
33	12877.0
34	14589.0
35	16301.0
36	17606.0
37	18911.0
38	20694.0
39	22477.0
40	23934.5
41	25392.0
42	27527.5
43	29663.0
44	32173.0
45	34683.0
46	38784.5
47	42886.0
48	83005.0
49	123124.0
50	103445.0
51	83766.0
52	78216.5
53	72667.0
54	60771.0
55	48875.0
56	42335.5
57	35796.0
58	31980.0
59	28164.0
60	25958.5
61	23753.0
62	20899.5
63	18046.0
64	14935.5
65	11825.0
66	9553.5
67	7282.0
68	6080.0
69	4878.0
70	4037.5
71	3197.0
72	2797.0
73	2397.0
74	1945.5
75	1115.5
76	737.0
77	593.0
78	449.0
79	291.5
80	134.0
81	115.5
82	97.0
83	87.5
84	78.0
85	54.5
86	31.0
87	19.0
88	7.0
89	5.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	713320.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.70103731218518
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.54371899763699	21.249226674013077
2	11.841533845132496	7.034116771355678
3	4.323174433148083	3.852082954380486
4	2.1371678578121758	2.539044091491292
5	1.2925093384344937	1.9194434043595336
6	0.8606560152046923	1.533742585233071
7	0.621904260003195	1.2929841141673264
8	0.5170791558607285	1.228622984125817
9	0.4344870810960651	1.161424530656699
>10	6.235125269688122	39.46069133811312
>50	0.12285221518094326	2.4724073495825016
>100	0.05975302294689339	3.146519563610544
>500	0.0028681451014508827	0.6035490779220478
>1k	0.004780241835751472	3.761852827245961
>5k	9.560483671502942E-4	1.7694735728399156
>10k+	0.0014340725507254414	6.974818160902968
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGC	20870	2.9257556216004037	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCG	15208	2.132002467335838	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTC	13048	1.8291930690293274	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGGACTCT	6365	0.8923063982504346	No Hit
GCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	6098	0.8548757920708798	TruSeq Adapter, Index 13 (95% over 23bp)
GAACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCT	4442	0.6227219200358884	TruSeq Adapter, Index 8 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTA	4227	0.5925811697414904	No Hit
CCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	3138	0.43991476476195823	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTC	3125	0.438092300790669	No Hit
CTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCT	2760	0.38692312005831886	TruSeq Adapter, Index 8 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCT	2637	0.3696798070991981	No Hit
TCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGC	2098	0.29411764705882354	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAGGACTCTCGT	1528	0.21420961139460548	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTATG	1273	0.17846127965008693	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1268	0.17776033196882185	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCAGGACTCTCGTAT	889	0.12462849772892952	No Hit
TGTCTCTTATACACATCTGACGCCAGGACTCTCGTATGCCGTCTTCTGCTT	852	0.119441484887568	TruSeq Adapter, Index 13 (96% over 25bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCCAGGACTC	714	0.10009532888465204	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.4431391240957775	0.0
2	0.0	0.0	0.0	2.272612572197611	0.0
3	0.0	0.0	0.0	2.760612347894353	0.0
4	0.0	0.0	0.0	4.090450288790445	0.0
5	0.0	0.0	0.0	7.042561543206415	0.0
6	0.0	0.0	0.0	7.650002803790725	0.0
7	0.0	0.0	0.0	8.61254415970392	0.0
8	0.0	0.0	0.0	9.937896035439914	0.0
9	0.0	0.0	0.0	10.284444569057365	0.0
10	0.0	0.0	0.0	13.426232266023664	0.0
11	0.0	0.0	0.0	15.023832221163012	0.0
12	0.0	0.0	0.0	19.66887231537038	0.0
13	0.0	0.0	0.0	20.36463298379409	0.0
14	0.0	0.0	0.0	20.64837660517019	0.0
15	0.0	0.0	0.0	21.56507598272865	0.0
16	1.4018953625301406E-4	0.0	0.0	22.33864184377278	0.0
17	1.4018953625301406E-4	0.0	0.0	23.23206975831324	0.0
18	1.4018953625301406E-4	0.0	0.0	24.065356361801154	0.0
19	1.4018953625301406E-4	0.0	0.0	25.717910615151684	0.0
20	1.4018953625301406E-4	0.0	0.0	26.526664049795322	0.0
21	1.4018953625301406E-4	0.0	0.0	27.521589188582965	0.0
22	1.4018953625301406E-4	0.0	0.0	28.65670386362362	0.0
23	1.4018953625301406E-4	0.0	0.0	29.66004037458644	0.0
24	1.4018953625301406E-4	0.0	0.0	30.432344529804297	0.0
25	1.4018953625301406E-4	0.0	0.0	31.128665956373016	0.0
26	1.4018953625301406E-4	0.0	0.0	31.72741546570964	0.0
27	1.4018953625301406E-4	0.0	0.0	32.32462289014748	0.0
28	1.4018953625301406E-4	0.0	0.0	32.91888633432401	0.0
29	1.4018953625301406E-4	0.0	0.0	33.57511355352437	0.0
30	1.4018953625301406E-4	0.0	0.0	34.25475242527898	0.0
31	1.4018953625301406E-4	0.0	0.0	34.91911063758201	0.0
32	1.4018953625301406E-4	0.0	0.0	35.5931419278865	0.0
33	1.4018953625301406E-4	0.0	0.0	36.2137610048786	0.0
34	2.803790725060281E-4	0.0	0.0	36.80816463859138	0.0
35	2.803790725060281E-4	0.0	0.0	37.447989682050135	0.0
36	2.803790725060281E-4	0.0	0.0	38.08921662087142	0.0
37	2.803790725060281E-4	0.0	0.0	38.700583188470816	0.0
38	2.803790725060281E-4	0.0	0.0	39.3346604609432	0.0
39	2.803790725060281E-4	0.0	0.0	39.993130712723605	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	110	0.0	45.000004	6
TCGCAAT	20	7.0314854E-4	45.0	13
AGCGATA	20	7.0314854E-4	45.0	39
TTGCGAT	20	7.0314854E-4	45.0	27
ACGTCGT	20	7.0314854E-4	45.0	21
CGTAACC	25	3.8893126E-5	45.0	35
ACGTACC	20	7.0314854E-4	45.0	33
ATACGTT	25	3.8893126E-5	45.0	27
CGCAATA	20	7.0314854E-4	45.0	14
GCTCGTA	25	3.8893126E-5	45.0	32
AACGTCG	30	2.164339E-6	44.999996	20
CGAATCG	30	2.164339E-6	44.999996	16
TACGGCT	2295	0.0	43.333336	7
TACGGGG	130	0.0	43.269234	4
ACGGCTG	2350	0.0	42.31915	8
AGACACG	125	0.0	41.399998	24
ATGATAC	6890	0.0	41.342525	3
ATACGAA	60	3.6379788E-12	41.249996	19
ATACGGC	4980	0.0	41.20482	6
CTTTGCG	175	0.0	41.142857	1
>>END_MODULE