FastQCFastQC Report
Sat 18 Jun 2016
SRR3549568_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549568_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences479717
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGC101852.12312676015234No Hit
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC95651.9938838940458645Illumina Single End Adapter 1 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC86031.7933489953451724No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCG85581.78396846474067No Hit
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC74581.5546666055195042Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT67691.4110402591527922Illumina Single End Adapter 1 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC46720.9739075329829878Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGTGAGAT39230.8177738124769395No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCC31810.6630992856204804No Hit
GAACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT30670.6393352747557414No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTC23250.4846607478992823No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTA22540.4698603551677343No Hit
TGAATGATACGGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATG22510.46923498646076744No Hit
TGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCTT22360.4661081429259334Illumina Single End Adapter 1 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCT21990.45839526220667604No Hit
CGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG19630.40919959059195316Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG13380.2789144433071999Illumina Single End Adapter 1 (95% over 21bp)
TGAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGTGAGA12960.27015928140966444No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12430.25911110091991735No Hit
TGAACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC10610.22117206603059722No Hit
TGAATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTT10220.2130422728400286No Hit
TCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG10080.21012388554085015Illumina Single End Adapter 1 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT9830.20491247964946002No Hit
TGAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGTGAGATCGT9230.19240510551012366No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC8770.18281611866996583No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCC8740.18219074996299903No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTGTGAGATCGT8170.17030874453062952No Hit
CGTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT6780.1413333277745004No Hit
TTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG6710.13987413412491115Illumina Single End Adapter 1 (95% over 21bp)
TGAATGACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTC6680.13924876541794431No Hit
TGAATGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCT6460.13466272823352102No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATG6130.12778367245688604No Hit
TTTCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC5690.1186115980880394No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTAT5660.11798622938107257No Hit
TGCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT5640.11756931690976137No Hit
GGCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT4900.10214355547124658No Hit
TTGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT4830.10068436182165735No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCG302.1628475E-645.0000041
CGGGTCA302.1628475E-645.0000046
CATATTA302.1628475E-645.00000428
CATACGA302.1628475E-645.00000418
CTTCTCG351.2099554E-745.0000041
TCGAGGG351.2099554E-745.0000043
ACATACG302.1628475E-645.00000417
ACAACCC207.0291717E-445.013
CGGGTAC207.0291717E-445.06
GTACCAC207.0291717E-445.028
ATTAGTC253.8873965E-545.031
CGACGTT207.0291717E-445.027
CGGTTCG207.0291717E-445.030
CCGGATG207.0291717E-445.011
CGATTGA453.8380676E-1045.020
TACGGGT207.0291717E-445.04
TACGCTA551.8189894E-1245.019
GCCGATC207.0291717E-445.09
TGCGAAA207.0291717E-445.018
TCTAACC207.0291717E-445.018
Produced by FastQC (version 0.11.5)