##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549568_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	479717
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.089925935499473	31.0	23.0	33.0	16.0	34.0
2	28.409735323117587	31.0	26.0	33.0	16.0	34.0
3	28.805785494364386	31.0	28.0	34.0	16.0	34.0
4	32.36069390911725	35.0	30.0	37.0	22.0	37.0
5	33.801164019619904	35.0	33.0	37.0	28.0	37.0
6	33.238847070251836	35.0	33.0	37.0	28.0	37.0
7	33.57628977084406	35.0	32.0	36.0	29.0	37.0
8	33.652207447307475	35.0	35.0	37.0	28.0	37.0
9	34.74253153421705	37.0	33.0	39.0	28.0	39.0
10	34.1065315592318	35.0	32.0	39.0	27.0	39.0
11	34.493870344390544	37.0	33.0	39.0	27.0	39.0
12	34.499010875161815	37.0	33.0	39.0	27.0	39.0
13	34.932201693915374	37.0	34.0	39.0	30.0	39.0
14	34.66930085863123	37.0	33.0	39.0	27.0	40.0
15	35.00899280200618	37.0	33.0	39.0	27.0	40.0
16	34.268808485002616	36.0	32.0	39.0	27.0	40.0
17	34.43777477137562	36.0	32.0	38.0	27.0	40.0
18	34.31982606411697	36.0	32.0	39.0	27.0	40.0
19	33.87592684853778	36.0	32.0	39.0	25.0	40.0
20	35.27937304702564	37.0	33.0	39.0	29.0	40.0
21	34.504514119783124	37.0	32.0	39.0	26.0	40.0
22	35.614045364246	37.0	34.0	39.0	30.0	40.0
23	36.46370255796647	38.0	35.0	40.0	31.0	40.0
24	36.46232466224878	38.0	35.0	40.0	31.0	40.0
25	34.46841366889228	37.0	32.0	39.0	25.0	40.0
26	35.06546359624529	37.0	33.0	39.0	27.0	40.0
27	35.662073680941056	37.0	34.0	39.0	30.0	40.0
28	35.974113904656285	38.0	35.0	40.0	30.0	40.0
29	36.13362878530467	38.0	35.0	40.0	30.0	40.0
30	34.33724466716835	37.0	33.0	39.0	25.0	40.0
31	35.03398045097422	37.0	33.0	39.0	27.0	40.0
32	35.35391699689609	37.0	34.0	39.0	30.0	40.0
33	36.12214076215769	38.0	35.0	40.0	30.0	40.0
34	36.346787793636665	38.0	35.0	40.0	31.0	40.0
35	36.63778436036247	38.0	35.0	40.0	31.0	41.0
36	36.52661881901204	38.0	35.0	40.0	31.0	41.0
37	36.58077991816008	38.0	35.0	40.0	31.0	41.0
38	36.286796173577336	38.0	35.0	40.0	30.0	41.0
39	36.1379938588793	38.0	35.0	40.0	30.0	41.0
40	35.88041074216674	38.0	34.0	40.0	30.0	41.0
41	35.28934350877705	37.0	33.0	40.0	29.0	40.0
42	36.18115680703415	38.0	35.0	40.0	31.0	41.0
43	36.269317118217614	38.0	35.0	40.0	31.0	41.0
44	36.60692866836072	38.0	35.0	40.0	31.0	41.0
45	36.87645841193871	39.0	35.0	40.0	32.0	41.0
46	36.67863344430154	39.0	35.0	40.0	32.0	41.0
47	36.60464190345558	39.0	35.0	40.0	31.0	41.0
48	36.59903443071644	38.0	35.0	40.0	31.0	41.0
49	36.849286141621	39.0	35.0	40.0	32.0	41.0
50	36.68457027789301	39.0	35.0	40.0	31.0	41.0
51	35.845456800572	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	5.0
11	14.0
12	14.0
13	15.0
14	18.0
15	31.0
16	52.0
17	89.0
18	154.0
19	288.0
20	502.0
21	770.0
22	1099.0
23	1519.0
24	2143.0
25	2915.0
26	4057.0
27	5570.0
28	7483.0
29	10383.0
30	14732.0
31	20701.0
32	28656.0
33	38487.0
34	49779.0
35	67397.0
36	85591.0
37	87378.0
38	45980.0
39	3891.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.590571107548826	3.9692151831183806	47.6116126799759	18.828601029356893
2	21.53373759945968	13.497749714936097	43.81228932891684	21.15622335668738
3	17.994567630498814	16.326917745253972	46.12115059503832	19.557364029208888
4	18.620770162408252	7.065415651311086	51.61376394832787	22.700050237952794
5	22.93873262777846	4.485561278837315	47.422751330471925	25.152954762912298
6	21.443476049420802	12.419197151653997	46.746310845769486	19.391015953155716
7	64.94099646249768	4.201852342110036	23.09111413604271	7.766037059349575
8	61.43788942230523	8.698670257672752	17.600376888874063	12.263063431147948
9	56.729696883787724	6.730843393083839	18.76064429653316	17.778815426595262
10	32.80246478653039	21.31569237696392	25.360160261153975	20.52168257535172
11	25.858162208135216	22.459283285770567	29.26746394228264	22.415090563811578
12	21.75490966549028	17.79841031274689	36.79711163039875	23.649568391364078
13	21.412624526543773	21.602319700990375	37.08561506054611	19.899440711919738
14	17.98518709989431	25.29282889703721	31.34764871788992	25.37433528517855
15	13.704746756942113	26.339070743792693	38.21086182061507	21.74532067865012
16	14.380561872937585	24.72374337369741	30.923648734566424	29.972046018798583
17	14.462068261078928	26.28570594746486	31.66929668950652	27.582929101949695
18	14.627790968425133	23.901591980271704	35.819243428938314	25.651373622364854
19	15.933977741043156	24.995153392521008	31.647200328527024	27.423668537908807
20	18.13944471427946	26.13874430132766	36.50047840706083	19.22133257733205
21	21.133293170765263	27.257737374326947	30.796490430816505	20.81247902409129
22	16.533706331024334	25.87379642580938	29.539082417341888	28.05341482582439
23	17.36294523646233	26.098095335374815	29.512816931649283	27.02614249651357
24	23.205973521888946	22.105949966334318	26.979239843491058	27.708836668285674
25	17.43215270669999	26.778079576083396	24.916982304150157	30.872785413066456
26	21.444309874363427	23.42881323780479	29.360226967149384	25.7666499206824
27	21.971287238100796	23.100703122882866	31.71453169264379	23.21347794637255
28	20.68552917657702	21.605446544525208	32.3286437628852	25.38038051601257
29	24.713320561914628	18.479228378398098	32.992368417212646	23.815082642474625
30	27.24147778794581	22.057379663426563	31.179007623244537	19.522134925383092
31	26.892105136987016	26.964856363230822	24.891759099635827	21.251279400146338
32	27.948352883053968	26.344282149684084	26.245056981512015	19.462307985749934
33	24.44191054309103	25.98448668694251	25.34973744937119	24.223865320595266
34	27.060120862925434	23.854063958542223	27.003003854355796	22.082811324176546
35	26.1737649489178	21.38781823450076	27.590641982668952	24.847774833912496
36	28.873898569364854	24.924069816162444	27.06533226881682	19.136699345655877
37	22.08260286794089	23.141143632600052	37.312623901175066	17.46362959828399
38	27.607318481521396	23.973509381572885	28.156392206238262	20.262779930667456
39	26.078500449223185	23.958708988841337	31.488565133193113	18.474225428742365
40	28.629796317412143	22.4624101293054	29.06817978099588	19.839613772286576
41	24.014992172468354	27.968990050383873	23.562016772388723	24.454001004759057
42	26.14708255075388	22.138677595332247	28.655853346869094	23.05838650704478
43	27.344663624595334	21.273167304890176	28.147220131869418	23.234948938645076
44	22.466162341547204	19.052899938922323	31.32346779455387	27.157469924976603
45	24.253883018529677	16.36735825497116	29.180329235778597	30.198429490720567
46	27.655263415722185	19.528597068688413	32.200860090428314	20.615279425161084
47	19.385179178557358	19.812931374122662	39.23375656897712	21.568132878342855
48	21.033442633886228	20.793717962882283	31.8304333596683	26.342406043563184
49	23.74858510330049	17.006485073491245	37.601752700029394	21.643177123178877
50	25.36391247339577	16.07531106881766	31.584246545359036	26.976529912427537
51	20.912746473441636	16.356726986952726	28.226850413889853	34.503676125715785
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	157.0
1	310.5
2	464.0
3	1358.0
4	2252.0
5	1551.0
6	850.0
7	914.5
8	979.0
9	975.5
10	972.0
11	1040.5
12	1109.0
13	1082.5
14	1056.0
15	982.0
16	908.0
17	924.5
18	941.0
19	972.0
20	1003.0
21	1040.5
22	1078.0
23	1192.5
24	1307.0
25	1585.5
26	2173.0
27	2482.0
28	3115.0
29	3748.0
30	4053.0
31	4358.0
32	5111.0
33	5864.0
34	6464.5
35	7065.0
36	8105.0
37	9145.0
38	9874.5
39	10604.0
40	12081.5
41	13559.0
42	15979.0
43	18399.0
44	26469.0
45	34539.0
46	62226.5
47	89914.0
48	86207.0
49	82500.0
50	77981.0
51	73462.0
52	54920.5
53	36379.0
54	29431.5
55	22484.0
56	18499.5
57	14515.0
58	12545.0
59	10575.0
60	9307.0
61	8039.0
62	7033.5
63	6028.0
64	5168.5
65	4309.0
66	3397.0
67	2485.0
68	2026.0
69	1567.0
70	1286.5
71	1006.0
72	868.0
73	730.0
74	598.5
75	396.5
76	326.0
77	217.5
78	109.0
79	90.0
80	71.0
81	48.0
82	25.0
83	22.0
84	19.0
85	11.0
86	3.0
87	2.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	479717.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.467312370499066
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.08047685422588	22.42857420729182
2	13.095093797574311	8.503250016936601
3	5.348637932408957	5.209676955246657
4	2.8802762508598914	3.7405931499999214
5	1.7821356450086638	2.893057733654856
6	1.3403199058811603	2.6109951036384924
7	0.9813994118776979	2.2304380885960104
8	0.8161363883198401	2.119820404520894
9	0.6440362593475689	1.8819113769148743
>10	3.8098765846772262	20.215972956438645
>50	0.11663735253623043	2.649358267341491
>100	0.08229413206722926	5.3982278870575175
>500	0.00842380879428331	1.9879128299936273
>1k	0.01036776466988715	7.371624261842174
>5k	0.0032399264593397344	8.615831741637106
>10k+	6.479852918679469E-4	2.1427550188893103
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGC	10185	2.12312676015234	No Hit
CCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	9565	1.9938838940458645	Illumina Single End Adapter 1 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC	8603	1.7933489953451724	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCG	8558	1.78396846474067	No Hit
GCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	7458	1.5546666055195042	Illumina Single End Adapter 1 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCT	6769	1.4110402591527922	Illumina Single End Adapter 1 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGC	4672	0.9739075329829878	Illumina Single End Adapter 1 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGTGAGAT	3923	0.8177738124769395	No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCC	3181	0.6630992856204804	No Hit
GAACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT	3067	0.6393352747557414	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTC	2325	0.4846607478992823	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTA	2254	0.4698603551677343	No Hit
TGAATGATACGGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATG	2251	0.46923498646076744	No Hit
TGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTGCTT	2236	0.4661081429259334	Illumina Single End Adapter 1 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCT	2199	0.45839526220667604	No Hit
CGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	1963	0.40919959059195316	Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	1338	0.2789144433071999	Illumina Single End Adapter 1 (95% over 21bp)
TGAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGTGTGAGA	1296	0.27015928140966444	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1243	0.25911110091991735	No Hit
TGAACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC	1061	0.22117206603059722	No Hit
TGAATCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTT	1022	0.2130422728400286	No Hit
TCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	1008	0.21012388554085015	Illumina Single End Adapter 1 (95% over 21bp)
TTCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT	983	0.20491247964946002	No Hit
TGAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTGTGAGATCGT	923	0.19240510551012366	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC	877	0.18281611866996583	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCC	874	0.18219074996299903	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTGTGAGATCGT	817	0.17030874453062952	No Hit
CGTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT	678	0.1413333277745004	No Hit
TTCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCTG	671	0.13987413412491115	Illumina Single End Adapter 1 (95% over 21bp)
TGAATGACTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTC	668	0.13924876541794431	No Hit
TGAATGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCT	646	0.13466272823352102	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATG	613	0.12778367245688604	No Hit
TTTCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTC	569	0.1186115980880394	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTAT	566	0.11798622938107257	No Hit
TGCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT	564	0.11756931690976137	No Hit
GGCCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT	490	0.10214355547124658	No Hit
TTGCTGTCTCTTATACACATCTGACGCGTGTGAGATCGTATGCCGTCTTCT	483	0.10068436182165735	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.6793234344415562	0.0
2	0.0	0.0	0.0	6.54865264312084	0.0
3	0.0	0.0	0.0	8.254866931961969	0.0
4	0.0	0.0	0.0	10.847228678575076	0.0
5	0.0	0.0	0.0	15.697379913574045	0.0
6	0.0	0.0	0.0	17.49010354021225	0.0
7	0.0	0.0	0.0	19.493576421098272	0.0
8	0.0	0.0	0.0	22.21726559617441	0.0
9	0.0	0.0	0.0	23.124884046218916	0.0
10	0.0	0.0	0.0	26.643416847849878	0.0
11	0.0	0.0	0.0	30.636604498068653	0.0
12	0.0	0.0	0.0	35.54241354798767	0.0
13	0.0	0.0	0.0	37.22173698242922	0.0
14	0.0	0.0	0.0	37.679923788400245	0.0
15	0.0	0.0	0.0	38.77160909452865	0.0
16	0.0	0.0	0.0	40.20370343348266	0.0
17	0.0	0.0	0.0	41.85405145116808	0.0
18	0.0	0.0	0.0	43.49585276319163	0.0
19	2.0845623565560528E-4	0.0	0.0	45.49494806312889	0.0
20	2.0845623565560528E-4	0.0	0.0	47.13800011256637	0.0
21	2.0845623565560528E-4	0.0	0.0	48.524442535911795	0.0
22	2.0845623565560528E-4	0.0	0.0	50.0603480802223	0.0
23	2.0845623565560528E-4	0.0	0.0	51.402389325373086	0.0
24	2.0845623565560528E-4	0.0	0.0	52.474271289114206	0.0
25	2.0845623565560528E-4	0.0	0.0	53.419828774048035	0.0
26	2.0845623565560528E-4	0.0	0.0	54.2163400504881	0.0
27	4.1691247131121057E-4	0.0	0.0	55.00513844620891	0.0
28	4.1691247131121057E-4	0.0	0.0	55.81123870948914	0.0
29	4.1691247131121057E-4	0.0	0.0	56.63318164667919	0.0
30	4.1691247131121057E-4	0.0	0.0	57.511616223731906	0.0
31	4.1691247131121057E-4	0.0	0.0	58.32459554278877	0.0
32	4.1691247131121057E-4	0.0	0.0	59.110684007446054	0.0
33	4.1691247131121057E-4	0.0	0.0	59.83590325129191	0.0
34	4.1691247131121057E-4	0.0	0.0	60.54382062757834	0.0
35	4.1691247131121057E-4	0.0	0.0	61.277586577086076	0.0
36	4.1691247131121057E-4	0.0	0.0	61.976123422768005	0.0
37	4.1691247131121057E-4	0.0	0.0	62.6471440453434	0.0
38	4.1691247131121057E-4	0.0	0.0	63.36944490189007	0.0
39	4.1691247131121057E-4	0.0	0.0	64.04651075529948	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCG	30	2.1628475E-6	45.000004	1
CGGGTCA	30	2.1628475E-6	45.000004	6
CATATTA	30	2.1628475E-6	45.000004	28
CATACGA	30	2.1628475E-6	45.000004	18
CTTCTCG	35	1.2099554E-7	45.000004	1
TCGAGGG	35	1.2099554E-7	45.000004	3
ACATACG	30	2.1628475E-6	45.000004	17
ACAACCC	20	7.0291717E-4	45.0	13
CGGGTAC	20	7.0291717E-4	45.0	6
GTACCAC	20	7.0291717E-4	45.0	28
ATTAGTC	25	3.8873965E-5	45.0	31
CGACGTT	20	7.0291717E-4	45.0	27
CGGTTCG	20	7.0291717E-4	45.0	30
CCGGATG	20	7.0291717E-4	45.0	11
CGATTGA	45	3.8380676E-10	45.0	20
TACGGGT	20	7.0291717E-4	45.0	4
TACGCTA	55	1.8189894E-12	45.0	19
GCCGATC	20	7.0291717E-4	45.0	9
TGCGAAA	20	7.0291717E-4	45.0	18
TCTAACC	20	7.0291717E-4	45.0	18
>>END_MODULE