SRR3549566_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR3549566_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	551381
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGC	11083	2.010043871660431	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCG	9073	1.6455046510489117	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTC	7532	1.3660245819134138	No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	4933	0.8946626742669769	No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	4477	0.8119612391431696	No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	3453	0.6262457357072515	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCCATGGT	3424	0.6209862146138514	No Hit
TCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	2625	0.4760773403508644	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT	2287	0.41477671519330556	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTA	2238	0.4058899381734227	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTC	1778	0.3224630518643189	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT	1701	0.3084981165473602	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG	1107	0.2007686155308217	No Hit
TGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCTT	1056	0.19151911291829063	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1039	0.18843594538078026	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGT	759	0.13765436241002138	No Hit
TGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG	663	0.12024353396290406	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATG	654	0.11861126879598681	No Hit
TCCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG	651	0.11806718040701439	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT	608	0.11026858016507642	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT	557	0.10101907755254533	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCACG	20	7.0300885E-4	45.000004	22
ATAGCGG	20	7.0300885E-4	45.000004	2
CGTCTAA	20	7.0300885E-4	45.000004	22
ATAAGGT	20	7.0300885E-4	45.000004	37
CACAACG	20	7.0300885E-4	45.000004	12
ATTGCGA	20	7.0300885E-4	45.000004	10
TCGCGCG	20	7.0300885E-4	45.000004	1
CTAATCG	20	7.0300885E-4	45.000004	17
CTTACCG	20	7.0300885E-4	45.000004	1
ATAGCCG	35	1.2103919E-7	45.0	10
CTAGTGG	25	3.8881557E-5	45.0	2
GAACGTT	25	3.8881557E-5	45.0	9
TCGTACA	25	3.8881557E-5	45.0	34
CCGACCA	25	3.8881557E-5	45.0	22
GGCGATA	25	3.8881557E-5	45.0	8
TATCCTC	25	3.8881557E-5	45.0	45
TTTCGTG	125	0.0	43.2	1
CTTCGCG	85	0.0	42.35294	1
TACGGCT	1355	0.0	41.512913	7
TCTTGCG	120	0.0	41.249996	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content