##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549566_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	551381
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.380082012256498	31.0	25.0	33.0	16.0	34.0
2	28.696079480431862	31.0	28.0	33.0	16.0	34.0
3	29.581117231097917	31.0	30.0	34.0	16.0	34.0
4	32.891628474684474	35.0	32.0	37.0	28.0	37.0
5	33.90314319862309	35.0	33.0	37.0	28.0	37.0
6	33.23846305911883	35.0	33.0	37.0	28.0	37.0
7	33.600795094499084	35.0	32.0	35.0	29.0	37.0
8	33.767168255707034	35.0	35.0	37.0	28.0	37.0
9	34.821502735857784	37.0	33.0	39.0	28.0	39.0
10	33.51938133522918	35.0	31.0	39.0	27.0	39.0
11	33.832573120945405	35.0	32.0	39.0	27.0	39.0
12	34.027904479842434	37.0	32.0	39.0	26.0	39.0
13	34.44333047384658	37.0	33.0	39.0	27.0	39.0
14	34.30877197436981	37.0	32.0	39.0	25.0	40.0
15	34.79154522916096	37.0	32.0	39.0	27.0	40.0
16	34.40929593148839	36.0	32.0	39.0	27.0	40.0
17	34.30358499839494	36.0	32.0	38.0	27.0	40.0
18	34.23538533246521	36.0	32.0	39.0	26.0	40.0
19	34.05352741570711	36.0	32.0	39.0	25.0	40.0
20	35.14512469599061	37.0	33.0	39.0	28.0	40.0
21	34.31010136366687	37.0	32.0	39.0	25.0	40.0
22	35.26507623585143	37.0	33.0	39.0	27.0	40.0
23	36.13545080443468	38.0	34.0	39.0	30.0	40.0
24	36.254045750579	38.0	35.0	40.0	31.0	40.0
25	34.262716705871256	37.0	32.0	39.0	25.0	40.0
26	34.7304767483827	37.0	33.0	39.0	27.0	40.0
27	35.54170890908464	37.0	34.0	39.0	30.0	40.0
28	35.74474818682545	38.0	34.0	40.0	30.0	40.0
29	36.2213242748662	38.0	35.0	40.0	30.0	41.0
30	34.050299157932535	37.0	32.0	39.0	24.0	40.0
31	34.626655615626944	37.0	33.0	39.0	25.0	40.0
32	35.10896095440358	37.0	34.0	39.0	29.0	40.0
33	35.95310139449854	38.0	35.0	40.0	30.0	40.0
34	36.26931468440153	38.0	35.0	40.0	30.0	41.0
35	36.43362756424324	38.0	35.0	40.0	31.0	41.0
36	36.25115120034967	38.0	35.0	40.0	30.0	41.0
37	36.584724537116806	38.0	35.0	40.0	31.0	41.0
38	36.12512400681199	38.0	35.0	40.0	30.0	41.0
39	36.036145605307404	38.0	35.0	40.0	30.0	41.0
40	35.64835930146305	38.0	34.0	40.0	30.0	41.0
41	35.30890436921113	37.0	34.0	40.0	29.0	41.0
42	36.10970454186851	38.0	35.0	40.0	30.0	41.0
43	36.069008544001335	38.0	35.0	40.0	31.0	41.0
44	36.44927373268212	38.0	35.0	40.0	31.0	41.0
45	36.786361880442016	39.0	35.0	40.0	32.0	41.0
46	36.57482394206547	38.0	35.0	40.0	31.0	41.0
47	36.4746645241675	38.0	35.0	40.0	31.0	41.0
48	36.551007379652184	38.0	35.0	40.0	32.0	41.0
49	36.71654989925297	39.0	35.0	40.0	32.0	41.0
50	36.5510363976996	38.0	35.0	40.0	32.0	41.0
51	35.81204285240152	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	8.0
10	11.0
11	20.0
12	14.0
13	14.0
14	24.0
15	51.0
16	84.0
17	125.0
18	266.0
19	480.0
20	715.0
21	1068.0
22	1460.0
23	2075.0
24	2745.0
25	3823.0
26	5205.0
27	7099.0
28	9451.0
29	13021.0
30	17996.0
31	25507.0
32	35414.0
33	45989.0
34	55040.0
35	72025.0
36	90887.0
37	97207.0
38	57477.0
39	6076.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.153162695123697	3.962051648497137	48.63062020635459	18.254165450024576
2	18.755452219064495	12.072414537316302	48.381790449797876	20.79034279382133
3	18.476334875521644	12.056817336832426	49.32397017670177	20.142877610944158
4	18.68617163086867	3.8766297714284677	54.015100266421946	23.42209833128091
5	23.574261717396862	5.257888828233109	46.83621669952356	24.331632754846467
6	20.474408802624684	12.153483707273192	49.3399301027783	18.032177387323827
7	74.74613742584528	1.8130838748524158	18.089850756554902	5.350927942747393
8	73.72034944983595	8.277942112622668	10.258786574074914	7.742921863466459
9	69.02631755537459	4.401312341194202	12.540511914628905	14.031858188802298
10	38.54213329802804	22.8397423922841	21.56331103175481	17.054813277933047
11	31.223419015163746	23.310560211541564	25.609152292153702	19.856868481140992
12	25.335838558093226	19.363743037935656	31.751910203652283	23.548508200318835
13	24.32601050816042	21.469727828851557	34.94933630284685	19.254925360141172
14	21.726356185650214	24.395835184745213	29.47217985385786	24.405628775746717
15	18.044872783066516	23.509877924701794	37.440898398747876	21.004350893483817
16	20.505603203592436	23.304031150873897	29.57664482454056	26.61372082099311
17	20.265478861259275	24.19633610878866	31.609903134130484	23.92828189582158
18	19.22590731272931	22.9456582653374	33.164545024221006	24.66388939771229
19	20.891361871373878	25.12146773283809	30.242790375439128	23.744380020348906
20	22.71188162087558	25.71996496070775	33.17760314555634	18.390550272860327
21	24.314403289195674	27.2463142545717	28.8631635838014	19.576118872431223
22	20.669011083080484	23.940251840378977	30.00574920064348	25.384987875897064
23	20.546772558358015	27.027953447797437	29.282837094495456	23.14243689934909
24	23.67328580418984	22.107399420727226	28.019463855301506	26.19985091978142
25	19.87319113281016	26.355097473434885	25.732297630857794	28.039413762897166
26	18.241470054281887	23.01965428623765	31.225595368719635	27.513280290760832
27	19.645580823423366	24.32564778256777	28.328687423034165	27.7000839709747
28	15.501440927416796	23.3769389949962	32.6115698582287	28.510050219358302
29	19.683667010651437	21.616813056670434	29.905455574276225	28.794064358401904
30	19.960063912249424	25.096439666945358	29.030017356419606	25.91347906438561
31	21.741046572152467	23.362974059679242	27.212036686066444	27.683942682101847
32	23.64209140322209	25.99327869476823	25.13633948213667	25.228290419873012
33	22.494608990879264	23.63937096127723	25.0035819152274	28.862438132616102
34	21.92603662440309	21.774054601083463	32.15417288589922	24.145735888614226
35	20.62076857925826	21.818125760590227	28.180514018437343	29.380591641714172
36	21.571653720385722	24.056324030026424	28.44548506386691	25.926537185720942
37	18.94007954572247	23.182880802929372	29.68346751157548	28.193572139772684
38	21.58035913460928	26.558405168114245	23.362974059679242	28.49826163759723
39	21.936374303793567	23.421191517299288	29.264700814863044	25.377733364044104
40	23.14769642044249	23.736581420106965	29.643386333587845	23.472335825862697
41	22.424240225905496	22.568242286186866	28.94604638172153	26.0614711061861
42	23.95584904086285	20.111864572772728	28.893269807991206	27.03901657837321
43	25.140692189248448	20.142333522555184	26.619343067679154	28.09763122051721
44	20.214697278288515	20.171714295559696	29.75401763934557	29.859570786806216
45	20.53335171143003	18.929016415146695	28.29078259860242	32.24684927482086
46	23.696500242119335	22.00148354767393	28.82090605225788	25.481110157948862
47	17.333205170290597	21.43055346484554	35.51301187382228	25.723229491041582
48	20.10533551210506	21.536832063491488	29.699427437652005	28.658404986751446
49	21.094669566053238	18.47216353120619	34.51878102437335	25.914385878367224
50	22.900680291849014	18.090576207740202	29.983078851102956	29.02566464930783
51	19.78885743251944	18.321632410257155	28.39724256004469	33.492267597178724
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	109.0
1	326.5
2	544.0
3	1375.0
4	2206.0
5	1576.0
6	946.0
7	1018.5
8	1091.0
9	1090.0
10	1089.0
11	1102.5
12	1116.0
13	1118.0
14	1120.0
15	1144.0
16	1168.0
17	1176.5
18	1185.0
19	1210.0
20	1235.0
21	1330.0
22	1425.0
23	1664.5
24	1904.0
25	2212.0
26	3037.5
27	3555.0
28	4154.0
29	4753.0
30	5570.5
31	6388.0
32	7435.0
33	8482.0
34	9579.0
35	10676.0
36	11520.5
37	12365.0
38	13548.0
39	14731.0
40	16663.0
41	18595.0
42	20145.0
43	21695.0
44	25388.5
45	29082.0
46	32672.0
47	36262.0
48	61372.0
49	86482.0
50	79066.0
51	71650.0
52	67777.0
53	63904.0
54	52037.5
55	40171.0
56	34428.0
57	28685.0
58	25209.0
59	21733.0
60	19352.0
61	16971.0
62	14946.5
63	12922.0
64	10790.0
65	8658.0
66	7192.0
67	5726.0
68	4752.0
69	3778.0
70	3078.5
71	2379.0
72	1992.0
73	1605.0
74	1349.5
75	962.0
76	830.0
77	561.5
78	293.0
79	204.5
80	116.0
81	113.0
82	110.0
83	66.0
84	22.0
85	15.5
86	9.0
87	5.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	551381.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.04095857001406
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.99038637627	24.165808015038976
2	12.259359932898048	8.346407271413458
3	4.707146324266308	4.807073190220301
4	2.2808529339739168	3.105696809188045
5	1.411855056151249	2.4030449736654775
6	0.9499533455128637	1.940239348682979
7	0.6645921599268111	1.5836347927417318
8	0.5703930719621759	1.5533381545029985
9	0.5040606953864298	1.5442838323579744
>10	5.509068510993302	33.88023893892147
>50	0.08504732028304261	2.0020112998759974
>100	0.05490396625866938	3.1728745806929357
>500	0.0043061934320525	0.903342093024727
>1k	0.006459290148078752	5.518632283512927
>5k	0.001076548358013125	3.042595426911885
>10k+	5.382741790065625E-4	2.030778989248083
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGC	11083	2.010043871660431	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCG	9073	1.6455046510489117	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTC	7532	1.3660245819134138	No Hit
GCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	4933	0.8946626742669769	No Hit
CCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	4477	0.8119612391431696	No Hit
CTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCT	3453	0.6262457357072515	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTCCCATGGT	3424	0.6209862146138514	No Hit
TCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGC	2625	0.4760773403508644	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT	2287	0.41477671519330556	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTA	2238	0.4058899381734227	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTC	1778	0.3224630518643189	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCT	1701	0.3084981165473602	No Hit
CGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG	1107	0.2007686155308217	No Hit
TGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTGCTT	1056	0.19151911291829063	Illumina Single End Adapter 2 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1039	0.18843594538078026	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTCCCATGGTCGT	759	0.13765436241002138	No Hit
TGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG	663	0.12024353396290406	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATG	654	0.11861126879598681	No Hit
TCCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCTG	651	0.11806718040701439	No Hit
TTCCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTTCT	608	0.11026858016507642	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCCCATGGTCGTATGCCGTCTT	557	0.10101907755254533	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6966145006810173	0.0
2	0.0	0.0	0.0	3.221365988309354	0.0
3	0.0	0.0	0.0	4.079212014922531	0.0
4	0.0	0.0	0.0	5.5268498551818075	0.0
5	0.0	0.0	0.0	8.491224761099856	0.0
6	0.0	0.0	0.0	9.400033733480116	0.0
7	0.0	0.0	0.0	10.611718575721689	0.0
8	0.0	0.0	0.0	12.206260281003516	0.0
9	0.0	0.0	0.0	12.75107412116123	0.0
10	0.0	0.0	0.0	15.645624350494487	0.0
11	0.0	0.0	0.0	17.789332603045807	0.0
12	0.0	0.0	0.0	21.75247242832089	0.0
13	0.0	0.0	0.0	22.574408621261885	0.0
14	0.0	0.0	0.0	22.88925443568059	0.0
15	0.0	0.0	0.0	23.671109450633953	0.0
16	0.0	0.0	0.0	24.588261111645124	0.0
17	0.0	0.0	0.0	25.75333571523139	0.0
18	0.0	0.0	0.0	26.962844203917072	0.0
19	0.0	0.0	0.0	28.487742595410435	0.0
20	0.0	0.0	0.0	29.499202910510157	0.0
21	0.0	0.0	0.0	30.72467132527236	0.0
22	0.0	0.0	0.0	31.98532412252145	0.0
23	0.0	0.0	0.0	33.190479904095355	0.0
24	0.0	0.0	0.0	34.14843819427946	0.0
25	0.0	0.0	0.0	35.04164996617584	0.0
26	0.0	0.0	0.0	35.82967131620422	0.0
27	0.0	0.0	0.0	36.569812888003035	0.0
28	1.8136279632413883E-4	0.0	0.0	37.34296248873284	0.0
29	1.8136279632413883E-4	0.0	0.0	38.14766921602304	0.0
30	1.8136279632413883E-4	0.0	0.0	38.95491502246178	0.0
31	1.8136279632413883E-4	0.0	0.0	39.73404959547028	0.0
32	1.8136279632413883E-4	0.0	0.0	40.483440669881624	0.0
33	1.8136279632413883E-4	0.0	0.0	41.24915439596214	0.0
34	1.8136279632413883E-4	0.0	0.0	42.03354849006404	0.0
35	1.8136279632413883E-4	0.0	0.0	42.76843054076945	0.0
36	1.8136279632413883E-4	0.0	0.0	43.495513991232926	0.0
37	1.8136279632413883E-4	0.0	0.0	44.23529283743908	0.0
38	1.8136279632413883E-4	0.0	0.0	44.9837770978688	0.0
39	1.8136279632413883E-4	0.0	0.0	45.76291167087731	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCCACG	20	7.0300885E-4	45.000004	22
ATAGCGG	20	7.0300885E-4	45.000004	2
CGTCTAA	20	7.0300885E-4	45.000004	22
ATAAGGT	20	7.0300885E-4	45.000004	37
CACAACG	20	7.0300885E-4	45.000004	12
ATTGCGA	20	7.0300885E-4	45.000004	10
TCGCGCG	20	7.0300885E-4	45.000004	1
CTAATCG	20	7.0300885E-4	45.000004	17
CTTACCG	20	7.0300885E-4	45.000004	1
ATAGCCG	35	1.2103919E-7	45.0	10
CTAGTGG	25	3.8881557E-5	45.0	2
GAACGTT	25	3.8881557E-5	45.0	9
TCGTACA	25	3.8881557E-5	45.0	34
CCGACCA	25	3.8881557E-5	45.0	22
GGCGATA	25	3.8881557E-5	45.0	8
TATCCTC	25	3.8881557E-5	45.0	45
TTTCGTG	125	0.0	43.2	1
CTTCGCG	85	0.0	42.35294	1
TACGGCT	1355	0.0	41.512913	7
TCTTGCG	120	0.0	41.249996	1
>>END_MODULE