FastQCFastQC Report
Sat 18 Jun 2016
SRR3549563_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549563_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences504859
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203464.030036109091846No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCATCACGCATCGTATGCC7340.14538712789115377No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGTC253.8876864E-545.00000432
CTATCGG207.029524E-445.02
AACCCGA351.2101191E-745.024
CACGCGA207.029524E-445.021
GGATACG207.029524E-445.09
GACCCGA351.2101191E-745.040
CGTTTTT103800.043.937861
GCTTGCG1050.040.7142871
CTACGAA850.039.70588311
TACGAAT850.039.70588312
AGCTACG850.039.7058839
CGAATAT800.039.37514
CGATTCG750.039.010
GTTTTTT122650.038.891152
CTCGTAA356.241331E-638.57142633
CGCATGG356.241331E-638.5714262
GCTCGTA356.241331E-638.57142632
TGGGCGA8400.038.303576
GCGATTC1950.038.0769239
TACACGC1500.037.535
Produced by FastQC (version 0.11.5)